Experiment: nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3138 and Shewana3_3139 are separated by 64 nucleotides Shewana3_3139 and rpsT are separated by 83 nucleotides rpsT and Shewana3_3141 are separated by 60 nucleotides Shewana3_3141 and Shewana3_3142 are separated by 135 nucleotides
Shewana3_3138: Shewana3_3138 - integral membrane protein MviN (RefSeq), at 3,764,547 to 3,766,106
_3138
Shewana3_3139: Shewana3_3139 - hypothetical protein (RefSeq), at 3,766,171 to 3,766,275
_3139
Shewana3_3140: rpsT - 30S ribosomal protein S20 (RefSeq), at 3,766,359 to 3,766,625
rpsT
Shewana3_3141: Shewana3_3141 - ArsR family transcriptional regulator (RefSeq), at 3,766,686 to 3,766,982
_3141
Shewana3_3142: Shewana3_3142 - peptidase M28 (RefSeq), at 3,767,118 to 3,768,524
_3142
Position (kb)
3766
3767 Strain fitness (log2 ratio)
-1
0
1
2 at 3766.125 kb on - strand at 3766.176 kb on - strand at 3766.854 kb on + strand, within Shewana3_3141 at 3766.932 kb on + strand, within Shewana3_3141 at 3766.954 kb on + strand at 3766.962 kb on - strand at 3767.002 kb on + strand at 3767.026 kb on - strand at 3767.043 kb on - strand at 3767.045 kb on - strand at 3767.164 kb on + strand at 3767.183 kb on - strand at 3767.183 kb on - strand at 3767.240 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 300 mM remove 3,766,125 - -0.0 3,766,176 - +0.1 3,766,854 + Shewana3_3141 0.57 +0.1 3,766,932 + Shewana3_3141 0.83 +2.2 3,766,954 + -0.6 3,766,962 - -0.4 3,767,002 + -0.8 3,767,026 - +0.5 3,767,043 - +0.1 3,767,045 - -0.2 3,767,164 + +0.5 3,767,183 - +0.0 3,767,183 - +0.2 3,767,240 + -0.8
Or see this region's nucleotide sequence