Experiment: nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2599 and ribA are separated by 114 nucleotides ribA and Shewana3_2601 are separated by 263 nucleotides Shewana3_2601 and nrdG are separated by 125 nucleotides nrdG and Shewana3_2603 are separated by 105 nucleotides
Shewana3_2599: Shewana3_2599 - hypothetical protein (RefSeq), at 3,103,115 to 3,103,984
_2599
Shewana3_2600: ribA - GTP cyclohydrolase II (RefSeq), at 3,104,099 to 3,104,710
ribA
Shewana3_2601: Shewana3_2601 - hypothetical protein (RefSeq), at 3,104,974 to 3,105,330
_2601
Shewana3_2602: nrdG - anaerobic ribonucleotide reductase-activating protein (RefSeq), at 3,105,456 to 3,105,917
nrdG
Shewana3_2603: Shewana3_2603 - anaerobic ribonucleoside triphosphate reductase (RefSeq), at 3,106,023 to 3,108,140
_2603
Position (kb)
3104
3105
3106 Strain fitness (log2 ratio)
-1
0
1 at 3104.883 kb on - strand at 3105.109 kb on - strand, within Shewana3_2601 at 3105.114 kb on - strand, within Shewana3_2601 at 3105.138 kb on - strand, within Shewana3_2601 at 3105.226 kb on - strand, within Shewana3_2601 at 3105.602 kb on - strand, within nrdG at 3105.667 kb on + strand, within nrdG at 3105.668 kb on + strand, within nrdG at 3105.812 kb on + strand, within nrdG at 3105.859 kb on - strand, within nrdG at 3106.129 kb on - strand at 3106.286 kb on + strand, within Shewana3_2603 at 3106.286 kb on + strand, within Shewana3_2603 at 3106.301 kb on - strand, within Shewana3_2603
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 300 mM remove 3,104,883 - +1.1 3,105,109 - Shewana3_2601 0.38 +0.7 3,105,114 - Shewana3_2601 0.39 +0.5 3,105,138 - Shewana3_2601 0.46 +0.4 3,105,226 - Shewana3_2601 0.71 +0.5 3,105,602 - nrdG Shewana3_2602 0.32 -0.1 3,105,667 + nrdG Shewana3_2602 0.46 -0.3 3,105,668 + nrdG Shewana3_2602 0.46 -0.9 3,105,812 + nrdG Shewana3_2602 0.77 +0.4 3,105,859 - nrdG Shewana3_2602 0.87 -0.3 3,106,129 - +0.4 3,106,286 + Shewana3_2603 0.12 +0.4 3,106,286 + Shewana3_2603 0.12 -0.7 3,106,301 - Shewana3_2603 0.13 -0.3
Or see this region's nucleotide sequence