Experiment: nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2380 and Shewana3_2381 are separated by 24 nucleotides Shewana3_2381 and minC are separated by 123 nucleotides minC and Shewana3_2383 are separated by 27 nucleotides Shewana3_2383 and minE are separated by 2 nucleotides
Shewana3_2380: Shewana3_2380 - putative transglycosylase (RefSeq), at 2,860,378 to 2,861,379
_2380
Shewana3_2381: Shewana3_2381 - hypothetical protein (RefSeq), at 2,861,404 to 2,861,682
_2381
Shewana3_2382: minC - septum formation inhibitor (RefSeq), at 2,861,806 to 2,862,471
minC
Shewana3_2383: Shewana3_2383 - septum site-determining protein MinD (RefSeq), at 2,862,499 to 2,863,308
_2383
Shewana3_2384: minE - cell division topological specificity factor MinE (RefSeq), at 2,863,311 to 2,863,568
minE
Position (kb)
2861
2862
2863 Strain fitness (log2 ratio)
-2
-1
0
1 at 2860.930 kb on + strand, within Shewana3_2380 at 2860.968 kb on + strand at 2860.976 kb on - strand, within Shewana3_2380 at 2860.976 kb on - strand, within Shewana3_2380 at 2860.976 kb on - strand, within Shewana3_2380 at 2860.976 kb on - strand, within Shewana3_2380 at 2860.976 kb on - strand, within Shewana3_2380 at 2860.988 kb on - strand, within Shewana3_2380 at 2861.250 kb on - strand, within Shewana3_2380 at 2861.267 kb on + strand, within Shewana3_2380 at 2861.275 kb on - strand, within Shewana3_2380 at 2861.506 kb on - strand, within Shewana3_2381 at 2861.642 kb on + strand, within Shewana3_2381 at 2861.731 kb on + strand at 2861.739 kb on - strand at 2861.808 kb on + strand at 2861.847 kb on - strand at 2862.088 kb on - strand, within minC at 2862.088 kb on - strand, within minC at 2862.399 kb on + strand, within minC at 2862.490 kb on + strand at 2862.525 kb on + strand at 2862.926 kb on + strand, within Shewana3_2383 at 2863.079 kb on + strand, within Shewana3_2383
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 300 mM remove 2,860,930 + Shewana3_2380 0.55 +1.0 2,860,968 + +1.0 2,860,976 - Shewana3_2380 0.60 +0.8 2,860,976 - Shewana3_2380 0.60 +0.0 2,860,976 - Shewana3_2380 0.60 +0.7 2,860,976 - Shewana3_2380 0.60 -2.4 2,860,976 - Shewana3_2380 0.60 -0.4 2,860,988 - Shewana3_2380 0.61 -0.2 2,861,250 - Shewana3_2380 0.87 +0.3 2,861,267 + Shewana3_2380 0.89 +1.0 2,861,275 - Shewana3_2380 0.90 -0.5 2,861,506 - Shewana3_2381 0.37 -0.7 2,861,642 + Shewana3_2381 0.85 -1.0 2,861,731 + -0.1 2,861,739 - -1.0 2,861,808 + -1.0 2,861,847 - +1.0 2,862,088 - minC Shewana3_2382 0.42 +1.0 2,862,088 - minC Shewana3_2382 0.42 +1.0 2,862,399 + minC Shewana3_2382 0.89 -0.9 2,862,490 + -0.2 2,862,525 + -2.4 2,862,926 + Shewana3_2383 0.53 -2.5 2,863,079 + Shewana3_2383 0.72 -0.8
Or see this region's nucleotide sequence