Experiment: nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1765 and tmk overlap by 4 nucleotides tmk and Shewana3_1767 overlap by 7 nucleotides Shewana3_1767 and Shewana3_1768 are separated by 6 nucleotides
Shewana3_1765: Shewana3_1765 - aminodeoxychorismate lyase (RefSeq), at 2,079,266 to 2,080,276
_1765
Shewana3_1766: tmk - thymidylate kinase (RefSeq), at 2,080,273 to 2,080,902
tmk
Shewana3_1767: Shewana3_1767 - DNA polymerase III, delta prime subunit (RefSeq), at 2,080,896 to 2,081,852
_1767
Shewana3_1768: Shewana3_1768 - type IV pilus biogenesis protein pilZ (RefSeq), at 2,081,859 to 2,082,185
_1768
Position (kb)
2080
2081 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2079.374 kb on + strand, within Shewana3_1765 at 2079.380 kb on + strand, within Shewana3_1765 at 2079.398 kb on + strand, within Shewana3_1765 at 2079.482 kb on + strand, within Shewana3_1765 at 2079.520 kb on + strand, within Shewana3_1765 at 2079.626 kb on + strand, within Shewana3_1765 at 2079.769 kb on + strand, within Shewana3_1765 at 2080.078 kb on + strand, within Shewana3_1765 at 2081.887 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 300 mM remove 2,079,374 + Shewana3_1765 0.11 -0.4 2,079,380 + Shewana3_1765 0.11 +0.2 2,079,398 + Shewana3_1765 0.13 +2.2 2,079,482 + Shewana3_1765 0.21 -0.9 2,079,520 + Shewana3_1765 0.25 -1.4 2,079,626 + Shewana3_1765 0.36 -0.2 2,079,769 + Shewana3_1765 0.50 -0.6 2,080,078 + Shewana3_1765 0.80 -2.0 2,081,887 + +0.2
Or see this region's nucleotide sequence