Experiment: nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1503 and Shewana3_1504 overlap by 1 nucleotides Shewana3_1504 and Shewana3_1505 overlap by 1 nucleotides Shewana3_1505 and Shewana3_1506 are separated by 160 nucleotides
Shewana3_1503: Shewana3_1503 - exonuclease III (RefSeq), at 1,750,675 to 1,751,487
_1503
Shewana3_1504: Shewana3_1504 - YciI-like protein (RefSeq), at 1,751,487 to 1,751,786
_1504
Shewana3_1505: Shewana3_1505 - intracellular septation protein A (RefSeq), at 1,751,786 to 1,752,331
_1505
Shewana3_1506: Shewana3_1506 - ferric iron reductase (RefSeq), at 1,752,492 to 1,753,526
_1506
Position (kb)
1751
1752 Strain fitness (log2 ratio)
-2
-1
0
1 at 1750.514 kb on + strand at 1750.683 kb on + strand at 1750.919 kb on + strand, within Shewana3_1503 at 1750.919 kb on + strand, within Shewana3_1503 at 1750.919 kb on + strand, within Shewana3_1503 at 1750.919 kb on + strand, within Shewana3_1503 at 1750.927 kb on - strand, within Shewana3_1503 at 1751.052 kb on - strand, within Shewana3_1503 at 1751.182 kb on - strand, within Shewana3_1503 at 1751.262 kb on - strand, within Shewana3_1503 at 1751.276 kb on + strand, within Shewana3_1503 at 1751.284 kb on + strand, within Shewana3_1503 at 1751.568 kb on + strand, within Shewana3_1504 at 1751.576 kb on - strand, within Shewana3_1504 at 1751.576 kb on - strand, within Shewana3_1504 at 1751.576 kb on - strand, within Shewana3_1504 at 1751.666 kb on - strand, within Shewana3_1504 at 1752.398 kb on + strand at 1752.490 kb on + strand at 1752.498 kb on - strand at 1752.498 kb on - strand at 1752.498 kb on - strand at 1752.657 kb on - strand, within Shewana3_1506 at 1752.772 kb on + strand, within Shewana3_1506
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 300 mM remove 1,750,514 + +0.0 1,750,683 + -0.2 1,750,919 + Shewana3_1503 0.30 -1.9 1,750,919 + Shewana3_1503 0.30 -0.9 1,750,919 + Shewana3_1503 0.30 +0.1 1,750,919 + Shewana3_1503 0.30 -1.2 1,750,927 - Shewana3_1503 0.31 -1.2 1,751,052 - Shewana3_1503 0.46 -0.3 1,751,182 - Shewana3_1503 0.62 -1.7 1,751,262 - Shewana3_1503 0.72 -2.1 1,751,276 + Shewana3_1503 0.74 +0.1 1,751,284 + Shewana3_1503 0.75 +0.1 1,751,568 + Shewana3_1504 0.27 -0.9 1,751,576 - Shewana3_1504 0.30 -0.0 1,751,576 - Shewana3_1504 0.30 +0.2 1,751,576 - Shewana3_1504 0.30 -0.5 1,751,666 - Shewana3_1504 0.60 -0.1 1,752,398 + -0.8 1,752,490 + +0.7 1,752,498 - -0.3 1,752,498 - +0.8 1,752,498 - -0.1 1,752,657 - Shewana3_1506 0.16 -0.1 1,752,772 + Shewana3_1506 0.27 +0.5
Or see this region's nucleotide sequence