Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_640 and Pf6N2E2_641 overlap by 34 nucleotides Pf6N2E2_641 and Pf6N2E2_642 are separated by 31 nucleotides Pf6N2E2_642 and Pf6N2E2_643 are separated by 167 nucleotides Pf6N2E2_643 and Pf6N2E2_644 overlap by 4 nucleotides
Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280
_640
Pf6N2E2_641 - Extracytoplasmic function (ECF) sigma factor VreI, at 732,247 to 732,822
_641
Pf6N2E2_642 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 732,854 to 733,801
_642
Pf6N2E2_643 - General secretion pathway protein J, at 733,969 to 734,580
_643
Pf6N2E2_644 - General secretion pathway protein H, at 734,577 to 735,029
_644
Position (kb) 732
733
734 Strain fitness (log2 ratio) -2
-1
0
1
2 at 731.882 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.975 kb on + strand, within Pf6N2E2_640 at 731.976 kb on - strand, within Pf6N2E2_640 at 732.228 kb on + strand at 732.229 kb on - strand at 732.253 kb on - strand at 732.325 kb on + strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.326 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641 at 732.401 kb on - strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.454 kb on + strand, within Pf6N2E2_641 at 732.455 kb on - strand, within Pf6N2E2_641 at 732.477 kb on + strand, within Pf6N2E2_641 at 732.622 kb on + strand, within Pf6N2E2_641 at 732.623 kb on - strand, within Pf6N2E2_641 at 732.709 kb on - strand, within Pf6N2E2_641 at 733.517 kb on + strand, within Pf6N2E2_642 at 733.517 kb on + strand, within Pf6N2E2_642 at 733.518 kb on - strand, within Pf6N2E2_642 at 733.518 kb on - strand, within Pf6N2E2_642 at 733.927 kb on + strand at 733.970 kb on + strand at 733.997 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 731,882 + Pf6N2E2_640 0.37 -0.0 731,975 + Pf6N2E2_640 0.52 +0.6 731,975 + Pf6N2E2_640 0.52 +0.0 731,976 - Pf6N2E2_640 0.52 +0.1 732,228 + +2.3 732,229 - -0.4 732,253 - -0.7 732,325 + Pf6N2E2_641 0.14 +1.2 732,326 - Pf6N2E2_641 0.14 +0.6 732,326 - Pf6N2E2_641 0.14 +0.3 732,401 - Pf6N2E2_641 0.27 -1.0 732,401 - Pf6N2E2_641 0.27 +0.1 732,454 + Pf6N2E2_641 0.36 -0.1 732,454 + Pf6N2E2_641 0.36 -0.2 732,454 + Pf6N2E2_641 0.36 +0.3 732,454 + Pf6N2E2_641 0.36 -0.3 732,454 + Pf6N2E2_641 0.36 +0.4 732,455 - Pf6N2E2_641 0.36 +1.0 732,477 + Pf6N2E2_641 0.40 +1.0 732,622 + Pf6N2E2_641 0.65 +0.1 732,623 - Pf6N2E2_641 0.65 -0.1 732,709 - Pf6N2E2_641 0.80 +0.1 733,517 + Pf6N2E2_642 0.70 -0.6 733,517 + Pf6N2E2_642 0.70 -1.5 733,518 - Pf6N2E2_642 0.70 -2.1 733,518 - Pf6N2E2_642 0.70 -0.3 733,927 + -0.2 733,970 + +0.6 733,997 + +1.0
Or see this region's nucleotide sequence