Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_6029

Experiment: A22 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_6027 and Pf6N2E2_6028 are separated by 339 nucleotidesPf6N2E2_6028 and Pf6N2E2_6029 are separated by 14 nucleotidesPf6N2E2_6029 and Pf6N2E2_6030 overlap by 10 nucleotidesPf6N2E2_6030 and Pf6N2E2_6031 are separated by 185 nucleotides Pf6N2E2_6027 - hypothetical protein, at 6,845,334 to 6,846,344 _6027 Pf6N2E2_6028 - hypothetical protein, at 6,846,684 to 6,846,821 _6028 Pf6N2E2_6029 - hypothetical protein, at 6,846,836 to 6,847,201 _6029 Pf6N2E2_6030 - Diaminopimelate epimerase (EC 5.1.1.7), at 6,847,192 to 6,847,995 _6030 Pf6N2E2_6031 - hypothetical protein, at 6,848,181 to 6,848,705 _6031 Position (kb) 6846 6847 6848Strain fitness (log2 ratio) -2 -1 0 1 2at 6845.890 kb on + strand, within Pf6N2E2_6027at 6845.950 kb on + strand, within Pf6N2E2_6027at 6845.950 kb on + strand, within Pf6N2E2_6027at 6845.951 kb on - strand, within Pf6N2E2_6027at 6845.983 kb on + strand, within Pf6N2E2_6027at 6845.983 kb on + strand, within Pf6N2E2_6027at 6845.984 kb on - strand, within Pf6N2E2_6027at 6846.012 kb on + strand, within Pf6N2E2_6027at 6846.012 kb on + strand, within Pf6N2E2_6027at 6846.012 kb on + strand, within Pf6N2E2_6027at 6846.012 kb on + strand, within Pf6N2E2_6027at 6846.013 kb on - strand, within Pf6N2E2_6027at 6846.013 kb on - strand, within Pf6N2E2_6027at 6846.013 kb on - strand, within Pf6N2E2_6027at 6846.026 kb on - strand, within Pf6N2E2_6027at 6846.026 kb on - strand, within Pf6N2E2_6027at 6846.026 kb on - strand, within Pf6N2E2_6027at 6846.045 kb on + strand, within Pf6N2E2_6027at 6846.046 kb on - strand, within Pf6N2E2_6027at 6846.046 kb on - strand, within Pf6N2E2_6027at 6846.117 kb on + strand, within Pf6N2E2_6027at 6846.118 kb on - strand, within Pf6N2E2_6027at 6846.170 kb on + strand, within Pf6N2E2_6027at 6846.171 kb on - strand, within Pf6N2E2_6027at 6846.461 kb on + strandat 6846.461 kb on + strandat 6846.462 kb on - strandat 6846.747 kb on + strand, within Pf6N2E2_6028at 6846.866 kb on + strandat 6846.866 kb on + strandat 6846.866 kb on + strandat 6846.866 kb on + strandat 6846.866 kb on + strandat 6846.867 kb on - strandat 6846.867 kb on - strandat 6847.003 kb on + strand, within Pf6N2E2_6029at 6847.004 kb on - strand, within Pf6N2E2_6029at 6847.004 kb on - strand, within Pf6N2E2_6029at 6847.013 kb on - strand, within Pf6N2E2_6029at 6847.127 kb on + strand, within Pf6N2E2_6029at 6847.510 kb on - strand, within Pf6N2E2_6030at 6847.654 kb on + strand, within Pf6N2E2_6030at 6847.654 kb on + strand, within Pf6N2E2_6030at 6847.654 kb on + strand, within Pf6N2E2_6030at 6847.655 kb on - strand, within Pf6N2E2_6030at 6847.773 kb on + strand, within Pf6N2E2_6030at 6847.774 kb on - strand, within Pf6N2E2_6030at 6848.083 kb on + strandat 6848.192 kb on + strandat 6848.193 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.002 mg/ml
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6,845,890 + Pf6N2E2_6027 0.55 +0.7
6,845,950 + Pf6N2E2_6027 0.61 -0.2
6,845,950 + Pf6N2E2_6027 0.61 +0.8
6,845,951 - Pf6N2E2_6027 0.61 +0.6
6,845,983 + Pf6N2E2_6027 0.64 +0.1
6,845,983 + Pf6N2E2_6027 0.64 +0.2
6,845,984 - Pf6N2E2_6027 0.64 +0.1
6,846,012 + Pf6N2E2_6027 0.67 -1.2
6,846,012 + Pf6N2E2_6027 0.67 +1.6
6,846,012 + Pf6N2E2_6027 0.67 +0.8
6,846,012 + Pf6N2E2_6027 0.67 +1.6
6,846,013 - Pf6N2E2_6027 0.67 -0.4
6,846,013 - Pf6N2E2_6027 0.67 +0.9
6,846,013 - Pf6N2E2_6027 0.67 +0.0
6,846,026 - Pf6N2E2_6027 0.68 +0.1
6,846,026 - Pf6N2E2_6027 0.68 -1.2
6,846,026 - Pf6N2E2_6027 0.68 +0.8
6,846,045 + Pf6N2E2_6027 0.70 +0.4
6,846,046 - Pf6N2E2_6027 0.70 +0.9
6,846,046 - Pf6N2E2_6027 0.70 +0.2
6,846,117 + Pf6N2E2_6027 0.77 +0.7
6,846,118 - Pf6N2E2_6027 0.78 -0.4
6,846,170 + Pf6N2E2_6027 0.83 -0.4
6,846,171 - Pf6N2E2_6027 0.83 +2.4
6,846,461 + -1.0
6,846,461 + -0.2
6,846,462 - +1.4
6,846,747 + Pf6N2E2_6028 0.46 +0.2
6,846,866 + -0.5
6,846,866 + +2.6
6,846,866 + -2.1
6,846,866 + -0.5
6,846,866 + -0.1
6,846,867 - -0.2
6,846,867 - +0.3
6,847,003 + Pf6N2E2_6029 0.46 -1.0
6,847,004 - Pf6N2E2_6029 0.46 -1.0
6,847,004 - Pf6N2E2_6029 0.46 -1.1
6,847,013 - Pf6N2E2_6029 0.48 -1.2
6,847,127 + Pf6N2E2_6029 0.80 -1.0
6,847,510 - Pf6N2E2_6030 0.40 +0.9
6,847,654 + Pf6N2E2_6030 0.57 +0.4
6,847,654 + Pf6N2E2_6030 0.57 +1.5
6,847,654 + Pf6N2E2_6030 0.57 +0.3
6,847,655 - Pf6N2E2_6030 0.58 +1.0
6,847,773 + Pf6N2E2_6030 0.72 +0.2
6,847,774 - Pf6N2E2_6030 0.72 -0.8
6,848,083 + +0.1
6,848,192 + +1.4
6,848,193 - +1.0

Or see this region's nucleotide sequence