Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6020 and Pf6N2E2_6021 are separated by 144 nucleotides Pf6N2E2_6021 and Pf6N2E2_6022 overlap by 11 nucleotides Pf6N2E2_6022 and Pf6N2E2_6023 are separated by 4 nucleotides
Pf6N2E2_6020 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 6,840,119 to 6,841,075
_6020
Pf6N2E2_6021 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 6,841,220 to 6,841,507
_6021
Pf6N2E2_6022 - StbE replicon stabilization toxin, at 6,841,497 to 6,841,808
_6022
Pf6N2E2_6023 - Putative cytoplasmic protein, at 6,841,813 to 6,842,451
_6023
Position (kb)
6841
6842 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.647 kb on + strand, within Pf6N2E2_6020 at 6840.807 kb on - strand, within Pf6N2E2_6020 at 6841.033 kb on + strand at 6841.034 kb on - strand at 6841.034 kb on - strand at 6841.034 kb on - strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.135 kb on + strand at 6841.136 kb on - strand at 6841.198 kb on + strand at 6841.198 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.509 kb on + strand at 6841.653 kb on + strand, within Pf6N2E2_6022 at 6841.653 kb on + strand, within Pf6N2E2_6022 at 6841.692 kb on + strand, within Pf6N2E2_6022 at 6841.787 kb on + strand at 6841.788 kb on - strand at 6841.865 kb on + strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.866 kb on - strand at 6841.928 kb on - strand, within Pf6N2E2_6023 at 6842.161 kb on - strand, within Pf6N2E2_6023 at 6842.161 kb on - strand, within Pf6N2E2_6023 at 6842.194 kb on - strand, within Pf6N2E2_6023 at 6842.199 kb on + strand, within Pf6N2E2_6023 at 6842.199 kb on + strand, within Pf6N2E2_6023 at 6842.200 kb on - strand, within Pf6N2E2_6023 at 6842.218 kb on + strand, within Pf6N2E2_6023 at 6842.219 kb on - strand, within Pf6N2E2_6023 at 6842.332 kb on + strand, within Pf6N2E2_6023 at 6842.333 kb on - strand, within Pf6N2E2_6023 at 6842.362 kb on + strand, within Pf6N2E2_6023 at 6842.393 kb on - strand at 6842.523 kb on - strand at 6842.556 kb on + strand at 6842.559 kb on - strand at 6842.686 kb on + strand at 6842.686 kb on + strand at 6842.687 kb on - strand at 6842.705 kb on + strand at 6842.705 kb on + strand at 6842.707 kb on + strand at 6842.707 kb on + strand at 6842.707 kb on + strand at 6842.708 kb on - strand at 6842.708 kb on - strand at 6842.724 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 6,840,647 + Pf6N2E2_6020 0.55 +0.2 6,840,647 + Pf6N2E2_6020 0.55 +0.5 6,840,647 + Pf6N2E2_6020 0.55 -1.2 6,840,647 + Pf6N2E2_6020 0.55 -0.4 6,840,647 + Pf6N2E2_6020 0.55 +1.4 6,840,807 - Pf6N2E2_6020 0.72 +0.3 6,841,033 + +0.1 6,841,034 - +1.0 6,841,034 - +0.8 6,841,034 - -0.3 6,841,135 + +0.5 6,841,135 + -0.5 6,841,135 + +0.1 6,841,135 + +0.4 6,841,135 + +0.8 6,841,135 + +0.2 6,841,135 + +0.2 6,841,136 - -1.8 6,841,198 + -0.2 6,841,198 + -0.4 6,841,509 + -0.2 6,841,509 + -0.2 6,841,509 + -1.2 6,841,509 + -0.2 6,841,509 + +0.6 6,841,653 + Pf6N2E2_6022 0.50 +0.4 6,841,653 + Pf6N2E2_6022 0.50 -0.8 6,841,692 + Pf6N2E2_6022 0.62 -0.6 6,841,787 + -1.3 6,841,788 - -0.2 6,841,865 + -0.3 6,841,866 - +0.4 6,841,866 - -1.0 6,841,866 - -0.6 6,841,866 - -3.0 6,841,928 - Pf6N2E2_6023 0.18 -0.2 6,842,161 - Pf6N2E2_6023 0.54 +0.5 6,842,161 - Pf6N2E2_6023 0.54 +0.4 6,842,194 - Pf6N2E2_6023 0.60 +0.0 6,842,199 + Pf6N2E2_6023 0.60 +0.6 6,842,199 + Pf6N2E2_6023 0.60 -0.8 6,842,200 - Pf6N2E2_6023 0.61 +1.4 6,842,218 + Pf6N2E2_6023 0.63 +0.6 6,842,219 - Pf6N2E2_6023 0.64 +0.1 6,842,332 + Pf6N2E2_6023 0.81 +0.7 6,842,333 - Pf6N2E2_6023 0.81 +0.0 6,842,362 + Pf6N2E2_6023 0.86 +1.3 6,842,393 - -0.2 6,842,523 - +0.6 6,842,556 + +1.7 6,842,559 - +0.3 6,842,686 + +0.0 6,842,686 + -0.2 6,842,687 - -0.4 6,842,705 + +0.5 6,842,705 + +0.1 6,842,707 + +1.2 6,842,707 + -0.0 6,842,707 + +0.2 6,842,708 - -0.4 6,842,708 - +0.1 6,842,724 + +2.2
Or see this region's nucleotide sequence