Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5442 and Pf6N2E2_5443 overlap by 4 nucleotides Pf6N2E2_5443 and Pf6N2E2_5444 are separated by 88 nucleotides Pf6N2E2_5444 and Pf6N2E2_5445 are separated by 101 nucleotides Pf6N2E2_5445 and Pf6N2E2_5446 are separated by 233 nucleotides
Pf6N2E2_5442 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 6,248,785 to 6,251,199
_5442
Pf6N2E2_5443 - hypothetical protein, at 6,251,196 to 6,251,357
_5443
Pf6N2E2_5444 - Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-), at 6,251,446 to 6,252,627
_5444
Pf6N2E2_5445 - Putative preQ0 transporter, at 6,252,729 to 6,253,196
_5445
Pf6N2E2_5446 - iron-chelator utilization protein, at 6,253,430 to 6,254,215
_5446
Position (kb)
6251
6252
6253 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6250.543 kb on + strand, within Pf6N2E2_5442 at 6250.544 kb on - strand, within Pf6N2E2_5442 at 6250.544 kb on - strand, within Pf6N2E2_5442 at 6250.669 kb on + strand, within Pf6N2E2_5442 at 6250.669 kb on + strand, within Pf6N2E2_5442 at 6250.669 kb on + strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.837 kb on + strand, within Pf6N2E2_5442 at 6251.009 kb on - strand at 6251.020 kb on + strand at 6251.020 kb on + strand at 6251.020 kb on + strand at 6251.021 kb on - strand at 6251.021 kb on - strand at 6251.021 kb on - strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.117 kb on - strand at 6251.117 kb on - strand at 6251.117 kb on - strand at 6251.117 kb on - strand at 6251.117 kb on - strand at 6251.170 kb on + strand at 6251.170 kb on + strand at 6251.170 kb on + strand at 6251.170 kb on + strand at 6251.171 kb on - strand at 6251.171 kb on - strand at 6251.171 kb on - strand at 6251.171 kb on - strand at 6251.197 kb on + strand at 6251.447 kb on + strand at 6251.447 kb on + strand at 6251.447 kb on + strand at 6251.448 kb on - strand at 6251.448 kb on - strand at 6252.089 kb on + strand, within Pf6N2E2_5444 at 6252.090 kb on - strand, within Pf6N2E2_5444 at 6252.090 kb on - strand, within Pf6N2E2_5444 at 6252.233 kb on + strand, within Pf6N2E2_5444 at 6252.234 kb on - strand, within Pf6N2E2_5444 at 6252.545 kb on - strand at 6252.878 kb on + strand, within Pf6N2E2_5445 at 6252.878 kb on + strand, within Pf6N2E2_5445 at 6252.878 kb on + strand, within Pf6N2E2_5445 at 6252.879 kb on - strand, within Pf6N2E2_5445 at 6252.879 kb on - strand, within Pf6N2E2_5445 at 6253.012 kb on + strand, within Pf6N2E2_5445 at 6253.012 kb on + strand, within Pf6N2E2_5445 at 6253.012 kb on + strand, within Pf6N2E2_5445 at 6253.012 kb on + strand, within Pf6N2E2_5445 at 6253.012 kb on + strand, within Pf6N2E2_5445 at 6253.012 kb on + strand, within Pf6N2E2_5445 at 6253.013 kb on - strand, within Pf6N2E2_5445 at 6253.013 kb on - strand, within Pf6N2E2_5445 at 6253.013 kb on - strand, within Pf6N2E2_5445 at 6253.013 kb on - strand, within Pf6N2E2_5445 at 6253.013 kb on - strand, within Pf6N2E2_5445 at 6253.088 kb on + strand, within Pf6N2E2_5445 at 6253.089 kb on - strand, within Pf6N2E2_5445 at 6253.178 kb on - strand at 6253.226 kb on + strand at 6253.226 kb on + strand at 6253.227 kb on - strand at 6253.237 kb on - strand at 6253.242 kb on + strand at 6253.318 kb on + strand at 6253.318 kb on + strand at 6253.318 kb on + strand at 6253.319 kb on - strand at 6253.409 kb on - strand at 6253.409 kb on - strand at 6253.409 kb on - strand at 6253.409 kb on - strand at 6253.409 kb on - strand at 6253.409 kb on - strand at 6253.409 kb on - strand at 6253.409 kb on - strand at 6253.427 kb on - strand at 6253.427 kb on - strand at 6253.466 kb on + strand at 6253.466 kb on + strand at 6253.467 kb on - strand at 6253.467 kb on - strand at 6253.467 kb on - strand at 6253.467 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 6,250,543 + Pf6N2E2_5442 0.73 +0.4 6,250,544 - Pf6N2E2_5442 0.73 -0.3 6,250,544 - Pf6N2E2_5442 0.73 +0.6 6,250,669 + Pf6N2E2_5442 0.78 -0.2 6,250,669 + Pf6N2E2_5442 0.78 +0.0 6,250,669 + Pf6N2E2_5442 0.78 +0.5 6,250,670 - Pf6N2E2_5442 0.78 -0.4 6,250,670 - Pf6N2E2_5442 0.78 -0.2 6,250,670 - Pf6N2E2_5442 0.78 +0.0 6,250,670 - Pf6N2E2_5442 0.78 +0.7 6,250,670 - Pf6N2E2_5442 0.78 -0.7 6,250,749 - Pf6N2E2_5442 0.81 +0.7 6,250,749 - Pf6N2E2_5442 0.81 -0.3 6,250,749 - Pf6N2E2_5442 0.81 +0.4 6,250,749 - Pf6N2E2_5442 0.81 +0.5 6,250,749 - Pf6N2E2_5442 0.81 -1.0 6,250,837 + Pf6N2E2_5442 0.85 -0.5 6,251,009 - +0.2 6,251,020 + -0.7 6,251,020 + +0.2 6,251,020 + -0.1 6,251,021 - +0.0 6,251,021 - +1.3 6,251,021 - -2.0 6,251,116 + -0.2 6,251,116 + +1.9 6,251,116 + +0.8 6,251,116 + -0.3 6,251,116 + -0.2 6,251,116 + -0.6 6,251,116 + +1.4 6,251,116 + +1.6 6,251,116 + +1.6 6,251,117 - +0.3 6,251,117 - +0.2 6,251,117 - +0.0 6,251,117 - -1.3 6,251,117 - +1.3 6,251,170 + +0.3 6,251,170 + -1.1 6,251,170 + -0.3 6,251,170 + -0.6 6,251,171 - +0.5 6,251,171 - +0.6 6,251,171 - +0.0 6,251,171 - -0.0 6,251,197 + +0.4 6,251,447 + +0.2 6,251,447 + +0.6 6,251,447 + -0.2 6,251,448 - +0.6 6,251,448 - -1.0 6,252,089 + Pf6N2E2_5444 0.54 -0.4 6,252,090 - Pf6N2E2_5444 0.54 -0.6 6,252,090 - Pf6N2E2_5444 0.54 -0.3 6,252,233 + Pf6N2E2_5444 0.67 -1.8 6,252,234 - Pf6N2E2_5444 0.67 -0.3 6,252,545 - +0.6 6,252,878 + Pf6N2E2_5445 0.32 -1.1 6,252,878 + Pf6N2E2_5445 0.32 -0.3 6,252,878 + Pf6N2E2_5445 0.32 +0.9 6,252,879 - Pf6N2E2_5445 0.32 -0.3 6,252,879 - Pf6N2E2_5445 0.32 -1.4 6,253,012 + Pf6N2E2_5445 0.60 +0.4 6,253,012 + Pf6N2E2_5445 0.60 -0.6 6,253,012 + Pf6N2E2_5445 0.60 +1.8 6,253,012 + Pf6N2E2_5445 0.60 +0.2 6,253,012 + Pf6N2E2_5445 0.60 -0.6 6,253,012 + Pf6N2E2_5445 0.60 -0.7 6,253,013 - Pf6N2E2_5445 0.61 -2.1 6,253,013 - Pf6N2E2_5445 0.61 -0.1 6,253,013 - Pf6N2E2_5445 0.61 +1.1 6,253,013 - Pf6N2E2_5445 0.61 +0.4 6,253,013 - Pf6N2E2_5445 0.61 -0.3 6,253,088 + Pf6N2E2_5445 0.77 +1.7 6,253,089 - Pf6N2E2_5445 0.77 -0.5 6,253,178 - -0.1 6,253,226 + -0.3 6,253,226 + -0.3 6,253,227 - -0.1 6,253,237 - +0.8 6,253,242 + +0.1 6,253,318 + -0.4 6,253,318 + -1.0 6,253,318 + +1.4 6,253,319 - -0.2 6,253,409 - +0.5 6,253,409 - -0.1 6,253,409 - -1.0 6,253,409 - +0.6 6,253,409 - -0.4 6,253,409 - +0.5 6,253,409 - -0.1 6,253,409 - +0.8 6,253,427 - +0.5 6,253,427 - +0.7 6,253,466 + +0.0 6,253,466 + +0.2 6,253,467 - -0.6 6,253,467 - -0.4 6,253,467 - +0.0 6,253,467 - +0.0
Or see this region's nucleotide sequence