Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4526 and Pf6N2E2_4527 overlap by 4 nucleotides Pf6N2E2_4527 and Pf6N2E2_4528 are separated by 226 nucleotides Pf6N2E2_4528 and Pf6N2E2_4529 overlap by 30 nucleotides Pf6N2E2_4529 and Pf6N2E2_4530 overlap by 4 nucleotides
Pf6N2E2_4526 - Phosphinothricin N-acetyltransferase (EC 2.3.1.-), at 5,261,276 to 5,261,872
_4526
Pf6N2E2_4527 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,261,869 to 5,262,420
_4527
Pf6N2E2_4528 - hypothetical protein, at 5,262,647 to 5,262,760
_4528
Pf6N2E2_4529 - type III secretion protein HrpJ, at 5,262,731 to 5,263,858
_4529
Pf6N2E2_4530 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 5,263,855 to 5,265,942
_4530
Position (kb) 5262
5263
5264 Strain fitness (log2 ratio) -1
0
1
2 at 5261.758 kb on + strand, within Pf6N2E2_4526 at 5261.758 kb on + strand, within Pf6N2E2_4526 at 5261.759 kb on - strand, within Pf6N2E2_4526 at 5261.786 kb on - strand, within Pf6N2E2_4526 at 5261.786 kb on - strand, within Pf6N2E2_4526 at 5261.888 kb on + strand at 5262.158 kb on + strand, within Pf6N2E2_4527 at 5262.159 kb on - strand, within Pf6N2E2_4527 at 5262.159 kb on - strand, within Pf6N2E2_4527 at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.439 kb on - strand at 5262.514 kb on - strand at 5262.556 kb on + strand at 5262.738 kb on + strand at 5262.738 kb on + strand at 5262.739 kb on - strand at 5262.739 kb on - strand at 5262.739 kb on - strand at 5262.760 kb on + strand at 5262.892 kb on + strand, within Pf6N2E2_4529 at 5262.893 kb on - strand, within Pf6N2E2_4529 at 5262.893 kb on - strand, within Pf6N2E2_4529 at 5263.197 kb on + strand at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.305 kb on - strand, within Pf6N2E2_4529 at 5263.307 kb on + strand, within Pf6N2E2_4529 at 5263.307 kb on + strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.552 kb on + strand, within Pf6N2E2_4529 at 5263.552 kb on + strand, within Pf6N2E2_4529 at 5263.638 kb on + strand, within Pf6N2E2_4529 at 5263.638 kb on + strand, within Pf6N2E2_4529 at 5263.639 kb on - strand, within Pf6N2E2_4529 at 5263.645 kb on + strand, within Pf6N2E2_4529 at 5263.646 kb on - strand, within Pf6N2E2_4529 at 5263.706 kb on + strand, within Pf6N2E2_4529 at 5264.032 kb on + strand at 5264.033 kb on - strand at 5264.551 kb on + strand, within Pf6N2E2_4530 at 5264.552 kb on - strand, within Pf6N2E2_4530 at 5264.552 kb on - strand, within Pf6N2E2_4530 at 5264.552 kb on - strand, within Pf6N2E2_4530 at 5264.613 kb on + strand, within Pf6N2E2_4530 at 5264.614 kb on - strand, within Pf6N2E2_4530 at 5264.828 kb on - strand, within Pf6N2E2_4530
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 5,261,758 + Pf6N2E2_4526 0.81 -1.1 5,261,758 + Pf6N2E2_4526 0.81 -0.2 5,261,759 - Pf6N2E2_4526 0.81 +0.5 5,261,786 - Pf6N2E2_4526 0.85 +0.1 5,261,786 - Pf6N2E2_4526 0.85 +1.1 5,261,888 + +0.8 5,262,158 + Pf6N2E2_4527 0.52 +0.5 5,262,159 - Pf6N2E2_4527 0.53 +2.4 5,262,159 - Pf6N2E2_4527 0.53 -0.1 5,262,424 + -0.2 5,262,424 + -0.9 5,262,424 + +1.4 5,262,424 + +0.5 5,262,425 - +0.4 5,262,425 - +0.8 5,262,425 - -0.5 5,262,425 - -0.1 5,262,439 - -1.7 5,262,514 - +0.6 5,262,556 + +1.0 5,262,738 + -0.7 5,262,738 + -0.3 5,262,739 - -0.7 5,262,739 - -1.3 5,262,739 - +0.3 5,262,760 + +1.0 5,262,892 + Pf6N2E2_4529 0.14 -0.6 5,262,893 - Pf6N2E2_4529 0.14 -0.5 5,262,893 - Pf6N2E2_4529 0.14 -0.3 5,263,197 + +0.5 5,263,198 - Pf6N2E2_4529 0.41 -0.8 5,263,198 - Pf6N2E2_4529 0.41 +0.7 5,263,198 - Pf6N2E2_4529 0.41 -0.6 5,263,304 + Pf6N2E2_4529 0.51 +1.3 5,263,304 + Pf6N2E2_4529 0.51 +0.5 5,263,304 + Pf6N2E2_4529 0.51 -0.2 5,263,305 - Pf6N2E2_4529 0.51 -1.1 5,263,307 + Pf6N2E2_4529 0.51 +0.6 5,263,307 + Pf6N2E2_4529 0.51 -0.9 5,263,308 - Pf6N2E2_4529 0.51 -1.3 5,263,308 - Pf6N2E2_4529 0.51 -0.7 5,263,308 - Pf6N2E2_4529 0.51 -0.7 5,263,552 + Pf6N2E2_4529 0.73 -0.4 5,263,552 + Pf6N2E2_4529 0.73 +0.4 5,263,638 + Pf6N2E2_4529 0.80 -1.0 5,263,638 + Pf6N2E2_4529 0.80 +0.6 5,263,639 - Pf6N2E2_4529 0.80 +0.8 5,263,645 + Pf6N2E2_4529 0.81 +0.1 5,263,646 - Pf6N2E2_4529 0.81 -1.1 5,263,706 + Pf6N2E2_4529 0.86 +0.1 5,264,032 + +0.2 5,264,033 - +0.4 5,264,551 + Pf6N2E2_4530 0.33 +0.7 5,264,552 - Pf6N2E2_4530 0.33 +1.2 5,264,552 - Pf6N2E2_4530 0.33 +0.5 5,264,552 - Pf6N2E2_4530 0.33 +0.6 5,264,613 + Pf6N2E2_4530 0.36 +0.2 5,264,614 - Pf6N2E2_4530 0.36 -0.3 5,264,828 - Pf6N2E2_4530 0.47 -0.3
Or see this region's nucleotide sequence