Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4432 and Pf6N2E2_4433 overlap by 4 nucleotides Pf6N2E2_4433 and Pf6N2E2_4434 are separated by 396 nucleotides
Pf6N2E2_4432 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 5,163,293 to 5,165,209
_4432
Pf6N2E2_4433 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 5,165,206 to 5,166,453
_4433
Pf6N2E2_4434 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 5,166,850 to 5,167,392
_4434
Position (kb)
5165
5166
5167 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5164.262 kb on - strand, within Pf6N2E2_4432 at 5164.739 kb on + strand, within Pf6N2E2_4432 at 5164.739 kb on + strand, within Pf6N2E2_4432 at 5164.740 kb on - strand, within Pf6N2E2_4432 at 5164.740 kb on - strand, within Pf6N2E2_4432 at 5164.830 kb on - strand, within Pf6N2E2_4432 at 5164.831 kb on + strand, within Pf6N2E2_4432 at 5164.968 kb on - strand, within Pf6N2E2_4432 at 5165.129 kb on + strand at 5165.130 kb on - strand at 5165.130 kb on - strand at 5165.218 kb on + strand at 5165.218 kb on + strand at 5165.219 kb on - strand at 5165.287 kb on + strand at 5165.288 kb on - strand at 5165.302 kb on + strand at 5165.406 kb on + strand, within Pf6N2E2_4433 at 5165.406 kb on + strand, within Pf6N2E2_4433 at 5165.406 kb on + strand, within Pf6N2E2_4433 at 5165.407 kb on - strand, within Pf6N2E2_4433 at 5165.407 kb on - strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.566 kb on + strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.567 kb on - strand, within Pf6N2E2_4433 at 5165.569 kb on + strand, within Pf6N2E2_4433 at 5165.570 kb on - strand, within Pf6N2E2_4433 at 5165.608 kb on + strand, within Pf6N2E2_4433 at 5165.608 kb on + strand, within Pf6N2E2_4433 at 5165.609 kb on - strand, within Pf6N2E2_4433 at 5165.609 kb on - strand, within Pf6N2E2_4433 at 5166.028 kb on + strand, within Pf6N2E2_4433 at 5166.028 kb on + strand, within Pf6N2E2_4433 at 5166.028 kb on + strand, within Pf6N2E2_4433 at 5166.029 kb on - strand, within Pf6N2E2_4433 at 5166.077 kb on + strand, within Pf6N2E2_4433 at 5166.077 kb on + strand, within Pf6N2E2_4433 at 5166.078 kb on - strand, within Pf6N2E2_4433 at 5166.103 kb on + strand, within Pf6N2E2_4433 at 5166.103 kb on + strand, within Pf6N2E2_4433 at 5166.104 kb on - strand, within Pf6N2E2_4433 at 5166.127 kb on + strand, within Pf6N2E2_4433 at 5166.271 kb on + strand, within Pf6N2E2_4433 at 5166.482 kb on + strand at 5166.482 kb on + strand at 5166.482 kb on + strand at 5166.483 kb on - strand at 5166.483 kb on - strand at 5166.483 kb on - strand at 5166.508 kb on - strand at 5166.508 kb on - strand at 5166.537 kb on + strand at 5166.537 kb on + strand at 5166.537 kb on + strand at 5166.537 kb on + strand at 5166.537 kb on + strand at 5166.538 kb on - strand at 5166.538 kb on - strand at 5166.564 kb on - strand at 5166.588 kb on - strand at 5166.614 kb on + strand at 5166.615 kb on - strand at 5166.703 kb on - strand at 5166.711 kb on - strand at 5166.728 kb on - strand at 5166.755 kb on + strand at 5166.755 kb on + strand at 5166.756 kb on - strand at 5166.756 kb on - strand at 5166.818 kb on + strand at 5166.839 kb on - strand at 5166.839 kb on - strand at 5166.925 kb on + strand, within Pf6N2E2_4434 at 5166.925 kb on + strand, within Pf6N2E2_4434 at 5166.925 kb on + strand, within Pf6N2E2_4434 at 5166.934 kb on + strand, within Pf6N2E2_4434 at 5166.934 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.999 kb on - strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.023 kb on - strand, within Pf6N2E2_4434 at 5167.023 kb on - strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.055 kb on - strand, within Pf6N2E2_4434 at 5167.055 kb on - strand, within Pf6N2E2_4434 at 5167.055 kb on - strand, within Pf6N2E2_4434 at 5167.230 kb on + strand, within Pf6N2E2_4434 at 5167.231 kb on - strand, within Pf6N2E2_4434 at 5167.257 kb on + strand, within Pf6N2E2_4434 at 5167.309 kb on + strand, within Pf6N2E2_4434 at 5167.309 kb on + strand, within Pf6N2E2_4434 at 5167.310 kb on - strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.363 kb on - strand at 5167.400 kb on + strand at 5167.400 kb on + strand at 5167.400 kb on + strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.409 kb on + strand at 5167.410 kb on - strand at 5167.410 kb on - strand at 5167.420 kb on + strand at 5167.420 kb on + strand at 5167.421 kb on - strand at 5167.447 kb on + strand at 5167.447 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 5,164,262 - Pf6N2E2_4432 0.51 -2.2 5,164,739 + Pf6N2E2_4432 0.75 -3.4 5,164,739 + Pf6N2E2_4432 0.75 -1.5 5,164,740 - Pf6N2E2_4432 0.75 +0.5 5,164,740 - Pf6N2E2_4432 0.75 -2.5 5,164,830 - Pf6N2E2_4432 0.80 -1.7 5,164,831 + Pf6N2E2_4432 0.80 -3.7 5,164,968 - Pf6N2E2_4432 0.87 -3.4 5,165,129 + +0.1 5,165,130 - -1.3 5,165,130 - +0.5 5,165,218 + -0.9 5,165,218 + -1.0 5,165,219 - -0.1 5,165,287 + -0.9 5,165,288 - +0.5 5,165,302 + -2.0 5,165,406 + Pf6N2E2_4433 0.16 -2.5 5,165,406 + Pf6N2E2_4433 0.16 -0.5 5,165,406 + Pf6N2E2_4433 0.16 -0.3 5,165,407 - Pf6N2E2_4433 0.16 +0.1 5,165,407 - Pf6N2E2_4433 0.16 +0.9 5,165,566 + Pf6N2E2_4433 0.29 +0.1 5,165,566 + Pf6N2E2_4433 0.29 -0.6 5,165,566 + Pf6N2E2_4433 0.29 -1.0 5,165,566 + Pf6N2E2_4433 0.29 -1.3 5,165,567 - Pf6N2E2_4433 0.29 -0.7 5,165,567 - Pf6N2E2_4433 0.29 -1.5 5,165,567 - Pf6N2E2_4433 0.29 +0.0 5,165,567 - Pf6N2E2_4433 0.29 -0.5 5,165,569 + Pf6N2E2_4433 0.29 -1.2 5,165,570 - Pf6N2E2_4433 0.29 -2.3 5,165,608 + Pf6N2E2_4433 0.32 -1.0 5,165,608 + Pf6N2E2_4433 0.32 -1.0 5,165,609 - Pf6N2E2_4433 0.32 -0.5 5,165,609 - Pf6N2E2_4433 0.32 -1.8 5,166,028 + Pf6N2E2_4433 0.66 -2.3 5,166,028 + Pf6N2E2_4433 0.66 -1.0 5,166,028 + Pf6N2E2_4433 0.66 -1.0 5,166,029 - Pf6N2E2_4433 0.66 -1.8 5,166,077 + Pf6N2E2_4433 0.70 -1.0 5,166,077 + Pf6N2E2_4433 0.70 +0.2 5,166,078 - Pf6N2E2_4433 0.70 -1.3 5,166,103 + Pf6N2E2_4433 0.72 -0.4 5,166,103 + Pf6N2E2_4433 0.72 -1.4 5,166,104 - Pf6N2E2_4433 0.72 -0.4 5,166,127 + Pf6N2E2_4433 0.74 +0.3 5,166,271 + Pf6N2E2_4433 0.85 +0.2 5,166,482 + -0.3 5,166,482 + -1.1 5,166,482 + +0.4 5,166,483 - +1.1 5,166,483 - -0.5 5,166,483 - -1.2 5,166,508 - -1.0 5,166,508 - -0.2 5,166,537 + +0.5 5,166,537 + -0.5 5,166,537 + +1.0 5,166,537 + -1.8 5,166,537 + -0.9 5,166,538 - +0.3 5,166,538 - +2.9 5,166,564 - +1.2 5,166,588 - -0.4 5,166,614 + -0.3 5,166,615 - -0.3 5,166,703 - +0.9 5,166,711 - +0.9 5,166,728 - +0.6 5,166,755 + +0.6 5,166,755 + +0.7 5,166,756 - +1.2 5,166,756 - -0.7 5,166,818 + -0.9 5,166,839 - -0.0 5,166,839 - +0.1 5,166,925 + Pf6N2E2_4434 0.14 +0.8 5,166,925 + Pf6N2E2_4434 0.14 -1.0 5,166,925 + Pf6N2E2_4434 0.14 -0.4 5,166,934 + Pf6N2E2_4434 0.15 -2.3 5,166,934 + Pf6N2E2_4434 0.15 -0.2 5,166,998 + Pf6N2E2_4434 0.27 +0.2 5,166,998 + Pf6N2E2_4434 0.27 -0.9 5,166,998 + Pf6N2E2_4434 0.27 +1.0 5,166,998 + Pf6N2E2_4434 0.27 +0.2 5,166,998 + Pf6N2E2_4434 0.27 +0.2 5,166,998 + Pf6N2E2_4434 0.27 +0.9 5,166,999 - Pf6N2E2_4434 0.27 +0.6 5,167,022 + Pf6N2E2_4434 0.32 +0.2 5,167,022 + Pf6N2E2_4434 0.32 -0.0 5,167,022 + Pf6N2E2_4434 0.32 +1.5 5,167,022 + Pf6N2E2_4434 0.32 +0.8 5,167,022 + Pf6N2E2_4434 0.32 -1.3 5,167,022 + Pf6N2E2_4434 0.32 +1.8 5,167,022 + Pf6N2E2_4434 0.32 +0.5 5,167,023 - Pf6N2E2_4434 0.32 +0.0 5,167,023 - Pf6N2E2_4434 0.32 -0.4 5,167,054 + Pf6N2E2_4434 0.38 +0.7 5,167,054 + Pf6N2E2_4434 0.38 +1.5 5,167,054 + Pf6N2E2_4434 0.38 +0.8 5,167,054 + Pf6N2E2_4434 0.38 -0.7 5,167,054 + Pf6N2E2_4434 0.38 +0.0 5,167,055 - Pf6N2E2_4434 0.38 -0.1 5,167,055 - Pf6N2E2_4434 0.38 +0.6 5,167,055 - Pf6N2E2_4434 0.38 +0.0 5,167,230 + Pf6N2E2_4434 0.70 -1.0 5,167,231 - Pf6N2E2_4434 0.70 -0.1 5,167,257 + Pf6N2E2_4434 0.75 +1.0 5,167,309 + Pf6N2E2_4434 0.85 +0.1 5,167,309 + Pf6N2E2_4434 0.85 +1.2 5,167,310 - Pf6N2E2_4434 0.85 +1.2 5,167,313 + Pf6N2E2_4434 0.85 +0.5 5,167,313 + Pf6N2E2_4434 0.85 +0.3 5,167,313 + Pf6N2E2_4434 0.85 +0.2 5,167,313 + Pf6N2E2_4434 0.85 +1.1 5,167,313 + Pf6N2E2_4434 0.85 +0.2 5,167,313 + Pf6N2E2_4434 0.85 +1.1 5,167,313 + Pf6N2E2_4434 0.85 +0.3 5,167,314 - Pf6N2E2_4434 0.85 -0.0 5,167,314 - Pf6N2E2_4434 0.85 +0.3 5,167,314 - Pf6N2E2_4434 0.85 +1.2 5,167,314 - Pf6N2E2_4434 0.85 +2.0 5,167,314 - Pf6N2E2_4434 0.85 +1.0 5,167,314 - Pf6N2E2_4434 0.85 +0.9 5,167,363 - -0.2 5,167,400 + +0.5 5,167,400 + -0.2 5,167,400 + +0.8 5,167,401 - -1.1 5,167,401 - +0.1 5,167,401 - -0.5 5,167,401 - +1.1 5,167,401 - +1.0 5,167,409 + +0.5 5,167,410 - +0.3 5,167,410 - +0.3 5,167,420 + +0.4 5,167,420 + -0.6 5,167,421 - +0.6 5,167,447 + -0.6 5,167,447 + +0.3
Or see this region's nucleotide sequence