Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3492 and Pf6N2E2_3493 are separated by 194 nucleotides Pf6N2E2_3493 and Pf6N2E2_3494 are separated by 2 nucleotides Pf6N2E2_3494 and Pf6N2E2_3495 are separated by 92 nucleotides
Pf6N2E2_3492 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 4,073,333 to 4,074,610
_3492
Pf6N2E2_3493 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 4,074,805 to 4,076,157
_3493
Pf6N2E2_3494 - Acyl dehydratase, at 4,076,160 to 4,077,014
_3494
Pf6N2E2_3495 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,077,107 to 4,077,661
_3495
Position (kb)
4074
4075
4076
4077 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4074.350 kb on + strand, within Pf6N2E2_3492 at 4074.357 kb on + strand, within Pf6N2E2_3492 at 4074.358 kb on - strand, within Pf6N2E2_3492 at 4074.358 kb on - strand, within Pf6N2E2_3492 at 4074.480 kb on + strand, within Pf6N2E2_3492 at 4074.481 kb on - strand, within Pf6N2E2_3492 at 4074.481 kb on - strand, within Pf6N2E2_3492 at 4074.540 kb on + strand at 4074.540 kb on + strand at 4074.540 kb on + strand at 4074.540 kb on + strand at 4074.541 kb on - strand at 4074.541 kb on - strand at 4074.599 kb on + strand at 4074.599 kb on + strand at 4074.599 kb on + strand at 4074.600 kb on - strand at 4074.600 kb on - strand at 4074.600 kb on - strand at 4074.627 kb on + strand at 4074.627 kb on + strand at 4074.627 kb on + strand at 4074.628 kb on - strand at 4074.633 kb on + strand at 4074.633 kb on + strand at 4074.633 kb on + strand at 4074.641 kb on + strand at 4074.641 kb on + strand at 4074.642 kb on - strand at 4074.726 kb on + strand at 4074.726 kb on + strand at 4074.726 kb on + strand at 4074.727 kb on - strand at 4074.733 kb on + strand at 4074.733 kb on + strand at 4074.734 kb on - strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.820 kb on - strand at 4074.939 kb on - strand at 4075.009 kb on + strand, within Pf6N2E2_3493 at 4075.010 kb on - strand, within Pf6N2E2_3493 at 4075.010 kb on - strand, within Pf6N2E2_3493 at 4075.010 kb on - strand, within Pf6N2E2_3493 at 4075.010 kb on - strand, within Pf6N2E2_3493 at 4075.078 kb on + strand, within Pf6N2E2_3493 at 4075.300 kb on + strand, within Pf6N2E2_3493 at 4075.300 kb on + strand, within Pf6N2E2_3493 at 4075.301 kb on - strand, within Pf6N2E2_3493 at 4075.605 kb on + strand, within Pf6N2E2_3493 at 4075.606 kb on - strand, within Pf6N2E2_3493 at 4075.870 kb on + strand, within Pf6N2E2_3493 at 4076.129 kb on + strand at 4076.130 kb on - strand at 4076.130 kb on - strand at 4076.212 kb on - strand at 4076.461 kb on + strand, within Pf6N2E2_3494 at 4076.461 kb on + strand, within Pf6N2E2_3494 at 4076.462 kb on - strand, within Pf6N2E2_3494 at 4076.718 kb on + strand, within Pf6N2E2_3494 at 4076.739 kb on + strand, within Pf6N2E2_3494 at 4076.739 kb on + strand, within Pf6N2E2_3494 at 4076.739 kb on + strand, within Pf6N2E2_3494 at 4076.740 kb on - strand, within Pf6N2E2_3494 at 4076.740 kb on - strand, within Pf6N2E2_3494 at 4076.984 kb on + strand at 4076.984 kb on + strand at 4076.984 kb on + strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.989 kb on + strand at 4076.989 kb on + strand at 4076.989 kb on + strand at 4076.989 kb on + strand at 4077.120 kb on + strand at 4077.121 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 4,074,350 + Pf6N2E2_3492 0.80 -0.9 4,074,357 + Pf6N2E2_3492 0.80 -0.0 4,074,358 - Pf6N2E2_3492 0.80 +1.0 4,074,358 - Pf6N2E2_3492 0.80 +0.5 4,074,480 + Pf6N2E2_3492 0.90 +0.5 4,074,481 - Pf6N2E2_3492 0.90 +0.6 4,074,481 - Pf6N2E2_3492 0.90 -0.3 4,074,540 + +0.1 4,074,540 + -1.3 4,074,540 + +0.7 4,074,540 + -1.0 4,074,541 - +1.1 4,074,541 - +0.7 4,074,599 + +1.2 4,074,599 + -0.7 4,074,599 + +0.3 4,074,600 - -0.5 4,074,600 - -0.6 4,074,600 - +0.7 4,074,627 + +0.9 4,074,627 + +1.1 4,074,627 + +0.4 4,074,628 - -0.3 4,074,633 + -0.1 4,074,633 + +0.7 4,074,633 + -0.4 4,074,641 + -0.5 4,074,641 + +2.9 4,074,642 - +0.9 4,074,726 + +0.4 4,074,726 + +0.2 4,074,726 + -1.9 4,074,727 - -0.5 4,074,733 + +0.7 4,074,733 + -0.7 4,074,734 - +0.7 4,074,817 + +0.5 4,074,817 + +0.3 4,074,817 + +0.6 4,074,817 + +1.3 4,074,817 + -0.1 4,074,817 + +0.8 4,074,820 - +0.3 4,074,939 - +0.1 4,075,009 + Pf6N2E2_3493 0.15 -0.8 4,075,010 - Pf6N2E2_3493 0.15 -0.9 4,075,010 - Pf6N2E2_3493 0.15 -1.1 4,075,010 - Pf6N2E2_3493 0.15 +1.7 4,075,010 - Pf6N2E2_3493 0.15 -0.6 4,075,078 + Pf6N2E2_3493 0.20 -2.4 4,075,300 + Pf6N2E2_3493 0.37 -0.1 4,075,300 + Pf6N2E2_3493 0.37 +1.0 4,075,301 - Pf6N2E2_3493 0.37 -0.8 4,075,605 + Pf6N2E2_3493 0.59 -0.5 4,075,606 - Pf6N2E2_3493 0.59 -0.7 4,075,870 + Pf6N2E2_3493 0.79 +0.5 4,076,129 + +0.6 4,076,130 - -0.7 4,076,130 - -0.1 4,076,212 - -0.0 4,076,461 + Pf6N2E2_3494 0.35 +0.5 4,076,461 + Pf6N2E2_3494 0.35 +1.8 4,076,462 - Pf6N2E2_3494 0.35 +0.2 4,076,718 + Pf6N2E2_3494 0.65 +0.9 4,076,739 + Pf6N2E2_3494 0.68 +0.2 4,076,739 + Pf6N2E2_3494 0.68 +0.2 4,076,739 + Pf6N2E2_3494 0.68 +1.8 4,076,740 - Pf6N2E2_3494 0.68 +0.3 4,076,740 - Pf6N2E2_3494 0.68 -1.7 4,076,984 + -0.8 4,076,984 + +0.6 4,076,984 + +0.5 4,076,985 - -0.4 4,076,985 - -0.3 4,076,985 - +0.5 4,076,985 - +1.4 4,076,985 - -0.8 4,076,985 - +0.6 4,076,985 - -0.0 4,076,985 - -0.1 4,076,989 + +0.6 4,076,989 + +1.3 4,076,989 + -0.1 4,076,989 + -0.1 4,077,120 + +0.1 4,077,121 - -0.4
Or see this region's nucleotide sequence