Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3343 and Pf6N2E2_3344 overlap by 4 nucleotides Pf6N2E2_3344 and Pf6N2E2_3345 are separated by 64 nucleotides
Pf6N2E2_3343 - AmpE protein, at 3,870,841 to 3,871,677
_3343
Pf6N2E2_3344 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 3,871,674 to 3,872,234
_3344
Pf6N2E2_3345 - Thymidine phosphorylase (EC 2.4.2.4), at 3,872,299 to 3,874,512
_3345
Position (kb)
3871
3872
3873 Strain fitness (log2 ratio)
-1
0
1 at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.691 kb on - strand at 3870.691 kb on - strand at 3870.691 kb on - strand at 3870.691 kb on - strand at 3870.875 kb on + strand at 3870.991 kb on - strand, within Pf6N2E2_3343 at 3871.008 kb on + strand, within Pf6N2E2_3343 at 3871.009 kb on - strand, within Pf6N2E2_3343 at 3871.009 kb on - strand, within Pf6N2E2_3343 at 3871.250 kb on + strand, within Pf6N2E2_3343 at 3871.251 kb on - strand, within Pf6N2E2_3343 at 3871.421 kb on + strand, within Pf6N2E2_3343 at 3871.421 kb on + strand, within Pf6N2E2_3343 at 3871.464 kb on - strand, within Pf6N2E2_3343 at 3871.464 kb on - strand, within Pf6N2E2_3343 at 3871.778 kb on + strand, within Pf6N2E2_3344 at 3871.779 kb on - strand, within Pf6N2E2_3344 at 3871.843 kb on + strand, within Pf6N2E2_3344 at 3871.844 kb on - strand, within Pf6N2E2_3344 at 3871.844 kb on - strand, within Pf6N2E2_3344 at 3871.844 kb on - strand, within Pf6N2E2_3344 at 3871.907 kb on + strand, within Pf6N2E2_3344 at 3871.908 kb on - strand, within Pf6N2E2_3344 at 3871.908 kb on - strand, within Pf6N2E2_3344 at 3872.045 kb on - strand, within Pf6N2E2_3344 at 3872.126 kb on + strand, within Pf6N2E2_3344 at 3872.234 kb on + strand at 3872.235 kb on - strand at 3872.271 kb on + strand at 3872.272 kb on - strand at 3872.272 kb on - strand at 3872.465 kb on + strand at 3872.465 kb on + strand at 3872.465 kb on + strand at 3872.465 kb on + strand at 3872.466 kb on - strand at 3872.466 kb on - strand at 3872.466 kb on - strand at 3872.830 kb on + strand, within Pf6N2E2_3345 at 3872.931 kb on - strand, within Pf6N2E2_3345 at 3873.205 kb on + strand, within Pf6N2E2_3345
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 3,870,690 + +1.2 3,870,690 + +0.3 3,870,690 + -1.3 3,870,690 + +1.1 3,870,690 + +0.3 3,870,691 - -1.2 3,870,691 - +0.1 3,870,691 - +0.1 3,870,691 - +0.4 3,870,875 + +0.6 3,870,991 - Pf6N2E2_3343 0.18 +0.0 3,871,008 + Pf6N2E2_3343 0.20 -0.2 3,871,009 - Pf6N2E2_3343 0.20 +0.9 3,871,009 - Pf6N2E2_3343 0.20 -0.3 3,871,250 + Pf6N2E2_3343 0.49 +0.3 3,871,251 - Pf6N2E2_3343 0.49 +0.7 3,871,421 + Pf6N2E2_3343 0.69 +1.1 3,871,421 + Pf6N2E2_3343 0.69 +1.2 3,871,464 - Pf6N2E2_3343 0.74 -0.2 3,871,464 - Pf6N2E2_3343 0.74 +0.6 3,871,778 + Pf6N2E2_3344 0.19 +0.2 3,871,779 - Pf6N2E2_3344 0.19 -1.3 3,871,843 + Pf6N2E2_3344 0.30 +1.2 3,871,844 - Pf6N2E2_3344 0.30 -0.5 3,871,844 - Pf6N2E2_3344 0.30 -1.6 3,871,844 - Pf6N2E2_3344 0.30 -1.4 3,871,907 + Pf6N2E2_3344 0.42 -0.4 3,871,908 - Pf6N2E2_3344 0.42 -0.8 3,871,908 - Pf6N2E2_3344 0.42 +0.2 3,872,045 - Pf6N2E2_3344 0.66 +0.8 3,872,126 + Pf6N2E2_3344 0.81 -1.4 3,872,234 + +1.1 3,872,235 - +0.0 3,872,271 + -0.6 3,872,272 - +1.5 3,872,272 - -0.1 3,872,465 + -0.9 3,872,465 + +1.3 3,872,465 + +0.1 3,872,465 + +1.1 3,872,466 - +0.9 3,872,466 - +0.4 3,872,466 - -0.1 3,872,830 + Pf6N2E2_3345 0.24 +0.6 3,872,931 - Pf6N2E2_3345 0.29 +0.1 3,873,205 + Pf6N2E2_3345 0.41 -0.0
Or see this region's nucleotide sequence