Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3218 and Pf6N2E2_3219 are separated by 42 nucleotides Pf6N2E2_3219 and Pf6N2E2_3220 overlap by 6 nucleotides
Pf6N2E2_3218 - Large exoproteins involved in heme utilization or adhesion, at 3,717,553 to 3,725,181
_3218
Pf6N2E2_3219 - hypothetical protein, at 3,725,224 to 3,725,718
_3219
Pf6N2E2_3220 - hypothetical protein, at 3,725,713 to 3,727,221
_3220
Position (kb)
3725
3726 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3724.581 kb on + strand at 3724.710 kb on - strand at 3724.710 kb on - strand at 3724.768 kb on + strand at 3724.861 kb on + strand at 3724.861 kb on + strand at 3724.862 kb on - strand at 3724.971 kb on + strand at 3725.096 kb on - strand at 3725.122 kb on - strand at 3725.164 kb on + strand at 3725.165 kb on - strand at 3725.165 kb on - strand at 3725.235 kb on + strand at 3725.235 kb on + strand at 3725.238 kb on + strand at 3725.238 kb on + strand at 3725.238 kb on + strand at 3725.238 kb on + strand at 3725.476 kb on + strand, within Pf6N2E2_3219 at 3725.476 kb on + strand, within Pf6N2E2_3219 at 3725.477 kb on - strand, within Pf6N2E2_3219 at 3725.477 kb on - strand, within Pf6N2E2_3219 at 3725.477 kb on - strand, within Pf6N2E2_3219 at 3725.673 kb on + strand at 3725.791 kb on + strand at 3725.791 kb on + strand at 3725.792 kb on - strand at 3725.795 kb on - strand at 3725.795 kb on - strand at 3725.795 kb on - strand at 3725.911 kb on + strand, within Pf6N2E2_3220 at 3726.127 kb on + strand, within Pf6N2E2_3220 at 3726.128 kb on - strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.142 kb on + strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.143 kb on - strand, within Pf6N2E2_3220 at 3726.154 kb on + strand, within Pf6N2E2_3220 at 3726.154 kb on + strand, within Pf6N2E2_3220 at 3726.154 kb on + strand, within Pf6N2E2_3220 at 3726.155 kb on - strand, within Pf6N2E2_3220 at 3726.155 kb on - strand, within Pf6N2E2_3220 at 3726.160 kb on + strand, within Pf6N2E2_3220 at 3726.227 kb on - strand, within Pf6N2E2_3220 at 3726.359 kb on - strand, within Pf6N2E2_3220 at 3726.495 kb on + strand, within Pf6N2E2_3220 at 3726.499 kb on + strand, within Pf6N2E2_3220 at 3726.500 kb on - strand, within Pf6N2E2_3220 at 3726.500 kb on - strand, within Pf6N2E2_3220 at 3726.703 kb on + strand, within Pf6N2E2_3220 at 3726.704 kb on - strand, within Pf6N2E2_3220 at 3726.704 kb on - strand, within Pf6N2E2_3220 at 3726.704 kb on - strand, within Pf6N2E2_3220 at 3726.705 kb on + strand, within Pf6N2E2_3220 at 3726.705 kb on + strand, within Pf6N2E2_3220 at 3726.706 kb on - strand, within Pf6N2E2_3220 at 3726.706 kb on - strand, within Pf6N2E2_3220
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 3,724,581 + +0.0 3,724,710 - -0.1 3,724,710 - +1.6 3,724,768 + +0.5 3,724,861 + +0.5 3,724,861 + +0.5 3,724,862 - +1.5 3,724,971 + +3.9 3,725,096 - -1.0 3,725,122 - +0.4 3,725,164 + +0.5 3,725,165 - +0.7 3,725,165 - +0.8 3,725,235 + -1.9 3,725,235 + -0.0 3,725,238 + -0.1 3,725,238 + -0.2 3,725,238 + +0.8 3,725,238 + -1.1 3,725,476 + Pf6N2E2_3219 0.51 -1.0 3,725,476 + Pf6N2E2_3219 0.51 +0.5 3,725,477 - Pf6N2E2_3219 0.51 +1.0 3,725,477 - Pf6N2E2_3219 0.51 -0.6 3,725,477 - Pf6N2E2_3219 0.51 -2.5 3,725,673 + -0.5 3,725,791 + +2.8 3,725,791 + +0.4 3,725,792 - +0.6 3,725,795 - +0.3 3,725,795 - +0.1 3,725,795 - +1.1 3,725,911 + Pf6N2E2_3220 0.13 -2.2 3,726,127 + Pf6N2E2_3220 0.27 +0.0 3,726,128 - Pf6N2E2_3220 0.28 +0.4 3,726,142 + Pf6N2E2_3220 0.28 -0.2 3,726,142 + Pf6N2E2_3220 0.28 -0.1 3,726,142 + Pf6N2E2_3220 0.28 +0.7 3,726,142 + Pf6N2E2_3220 0.28 -0.7 3,726,142 + Pf6N2E2_3220 0.28 +0.8 3,726,143 - Pf6N2E2_3220 0.28 -0.5 3,726,143 - Pf6N2E2_3220 0.28 +0.0 3,726,143 - Pf6N2E2_3220 0.28 -0.3 3,726,143 - Pf6N2E2_3220 0.28 -0.5 3,726,143 - Pf6N2E2_3220 0.28 +0.1 3,726,143 - Pf6N2E2_3220 0.28 -0.0 3,726,143 - Pf6N2E2_3220 0.28 -0.2 3,726,154 + Pf6N2E2_3220 0.29 +0.1 3,726,154 + Pf6N2E2_3220 0.29 -0.3 3,726,154 + Pf6N2E2_3220 0.29 +0.7 3,726,155 - Pf6N2E2_3220 0.29 +0.3 3,726,155 - Pf6N2E2_3220 0.29 +1.1 3,726,160 + Pf6N2E2_3220 0.30 +1.2 3,726,227 - Pf6N2E2_3220 0.34 -0.2 3,726,359 - Pf6N2E2_3220 0.43 +0.2 3,726,495 + Pf6N2E2_3220 0.52 +0.4 3,726,499 + Pf6N2E2_3220 0.52 +0.8 3,726,500 - Pf6N2E2_3220 0.52 +0.3 3,726,500 - Pf6N2E2_3220 0.52 +0.5 3,726,703 + Pf6N2E2_3220 0.66 +1.4 3,726,704 - Pf6N2E2_3220 0.66 -0.6 3,726,704 - Pf6N2E2_3220 0.66 +1.3 3,726,704 - Pf6N2E2_3220 0.66 +0.5 3,726,705 + Pf6N2E2_3220 0.66 +0.4 3,726,705 + Pf6N2E2_3220 0.66 +0.7 3,726,706 - Pf6N2E2_3220 0.66 -0.9 3,726,706 - Pf6N2E2_3220 0.66 +1.5
Or see this region's nucleotide sequence