Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1703 and Pf6N2E2_1704 are separated by 4 nucleotides Pf6N2E2_1704 and Pf6N2E2_1705 overlap by 4 nucleotides Pf6N2E2_1705 and Pf6N2E2_1706 overlap by 4 nucleotides Pf6N2E2_1706 and Pf6N2E2_1707 are separated by 4 nucleotides
Pf6N2E2_1703 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 2,011,840 to 2,012,904
_1703
Pf6N2E2_1704 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 2,012,909 to 2,013,790
_1704
Pf6N2E2_1705 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,013,787 to 2,014,557
_1705
Pf6N2E2_1706 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,014,554 to 2,015,525
_1706
Pf6N2E2_1707 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 2,015,530 to 2,016,915
_1707
Position (kb)
2013
2014
2015 Strain fitness (log2 ratio)
-1
0
1 at 2012.815 kb on + strand at 2012.874 kb on + strand at 2012.875 kb on - strand at 2012.912 kb on + strand at 2012.958 kb on + strand at 2012.958 kb on + strand at 2012.958 kb on + strand at 2012.958 kb on + strand at 2012.958 kb on + strand at 2012.959 kb on - strand at 2012.982 kb on + strand at 2012.983 kb on - strand at 2013.027 kb on + strand, within Pf6N2E2_1704 at 2013.514 kb on + strand, within Pf6N2E2_1704 at 2013.705 kb on + strand at 2013.705 kb on + strand at 2013.705 kb on + strand at 2013.706 kb on - strand at 2013.706 kb on - strand at 2013.810 kb on - strand at 2013.875 kb on + strand, within Pf6N2E2_1705 at 2014.573 kb on + strand at 2014.574 kb on - strand at 2014.574 kb on - strand at 2014.611 kb on + strand at 2015.187 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.200 kb on - strand, within Pf6N2E2_1706 at 2015.515 kb on + strand at 2015.515 kb on + strand at 2015.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 2,012,815 + -0.0 2,012,874 + +1.4 2,012,875 - -0.1 2,012,912 + -0.3 2,012,958 + +0.2 2,012,958 + -0.6 2,012,958 + +0.1 2,012,958 + -0.4 2,012,958 + +0.2 2,012,959 - -0.6 2,012,982 + -0.0 2,012,983 - +0.4 2,013,027 + Pf6N2E2_1704 0.13 -0.6 2,013,514 + Pf6N2E2_1704 0.69 +1.6 2,013,705 + +0.1 2,013,705 + -0.1 2,013,705 + -0.9 2,013,706 - +0.2 2,013,706 - -1.2 2,013,810 - +1.1 2,013,875 + Pf6N2E2_1705 0.11 -0.7 2,014,573 + -0.1 2,014,574 - +0.6 2,014,574 - +0.5 2,014,611 + -0.6 2,015,187 + Pf6N2E2_1706 0.65 +0.6 2,015,199 + Pf6N2E2_1706 0.66 -0.7 2,015,199 + Pf6N2E2_1706 0.66 -0.4 2,015,199 + Pf6N2E2_1706 0.66 -0.2 2,015,200 - Pf6N2E2_1706 0.66 -0.6 2,015,515 + -0.2 2,015,515 + +0.5 2,015,516 - -0.2
Or see this region's nucleotide sequence