Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1691 and Pf6N2E2_1692 are separated by 241 nucleotides Pf6N2E2_1692 and Pf6N2E2_1693 are separated by 16 nucleotides Pf6N2E2_1693 and Pf6N2E2_1694 are separated by 139 nucleotides Pf6N2E2_1694 and Pf6N2E2_1695 are separated by 59 nucleotides
Pf6N2E2_1691 - hypothetical protein, at 2,001,093 to 2,001,335
_1691
Pf6N2E2_1692 - transporter, LysE family, at 2,001,577 to 2,002,218
_1692
Pf6N2E2_1693 - hypothetical protein, at 2,002,235 to 2,002,354
_1693
Pf6N2E2_1694 - FMN reductase (EC 1.5.1.29), at 2,002,494 to 2,003,057
_1694
Pf6N2E2_1695 - Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme, at 2,003,117 to 2,004,304
_1695
Position (kb)
2001
2002
2003 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2000.619 kb on + strand at 2000.712 kb on - strand at 2000.712 kb on - strand at 2000.750 kb on - strand at 2000.969 kb on - strand at 2001.017 kb on + strand at 2001.088 kb on + strand at 2001.089 kb on - strand at 2001.094 kb on + strand at 2001.094 kb on + strand at 2001.094 kb on + strand at 2001.094 kb on + strand at 2001.094 kb on + strand at 2001.095 kb on - strand at 2001.125 kb on + strand, within Pf6N2E2_1691 at 2001.126 kb on - strand, within Pf6N2E2_1691 at 2001.158 kb on + strand, within Pf6N2E2_1691 at 2001.202 kb on + strand, within Pf6N2E2_1691 at 2001.348 kb on + strand at 2001.349 kb on - strand at 2001.351 kb on + strand at 2001.351 kb on + strand at 2001.351 kb on + strand at 2001.395 kb on - strand at 2001.512 kb on + strand at 2001.603 kb on + strand at 2001.924 kb on - strand, within Pf6N2E2_1692 at 2001.924 kb on - strand, within Pf6N2E2_1692 at 2001.924 kb on - strand, within Pf6N2E2_1692 at 2001.925 kb on + strand, within Pf6N2E2_1692 at 2001.926 kb on - strand, within Pf6N2E2_1692 at 2002.084 kb on + strand, within Pf6N2E2_1692 at 2002.085 kb on - strand, within Pf6N2E2_1692 at 2002.460 kb on + strand at 2002.460 kb on + strand at 2002.460 kb on + strand at 2002.461 kb on - strand at 2002.461 kb on - strand at 2002.490 kb on + strand at 2002.490 kb on + strand at 2002.490 kb on + strand at 2002.491 kb on - strand at 2002.491 kb on - strand at 2002.491 kb on - strand at 2002.491 kb on - strand at 2002.491 kb on - strand at 2002.756 kb on - strand, within Pf6N2E2_1694
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 2,000,619 + -0.4 2,000,712 - +0.3 2,000,712 - +0.6 2,000,750 - +1.9 2,000,969 - -0.1 2,001,017 + +0.3 2,001,088 + +0.2 2,001,089 - +0.2 2,001,094 + +0.2 2,001,094 + -1.1 2,001,094 + +0.8 2,001,094 + -0.3 2,001,094 + +0.4 2,001,095 - -0.9 2,001,125 + Pf6N2E2_1691 0.13 +0.6 2,001,126 - Pf6N2E2_1691 0.14 +1.6 2,001,158 + Pf6N2E2_1691 0.27 -2.4 2,001,202 + Pf6N2E2_1691 0.45 -0.7 2,001,348 + +0.4 2,001,349 - +0.1 2,001,351 + -0.0 2,001,351 + +1.4 2,001,351 + +0.7 2,001,395 - +0.4 2,001,512 + -0.4 2,001,603 + -0.1 2,001,924 - Pf6N2E2_1692 0.54 -2.4 2,001,924 - Pf6N2E2_1692 0.54 +1.3 2,001,924 - Pf6N2E2_1692 0.54 -0.5 2,001,925 + Pf6N2E2_1692 0.54 -1.4 2,001,926 - Pf6N2E2_1692 0.54 -1.2 2,002,084 + Pf6N2E2_1692 0.79 -0.4 2,002,085 - Pf6N2E2_1692 0.79 -1.3 2,002,460 + -0.1 2,002,460 + +0.6 2,002,460 + -0.4 2,002,461 - +0.4 2,002,461 - +1.0 2,002,490 + -0.1 2,002,490 + +0.3 2,002,490 + +0.8 2,002,491 - -0.8 2,002,491 - +0.4 2,002,491 - -0.4 2,002,491 - -0.2 2,002,491 - -0.0 2,002,756 - Pf6N2E2_1694 0.46 -0.1
Or see this region's nucleotide sequence