Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1091

Experiment: A22 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1090 and Pf6N2E2_1091 are separated by 193 nucleotidesPf6N2E2_1091 and Pf6N2E2_1092 overlap by 4 nucleotides Pf6N2E2_1090 - Transcriptional regulator for ferulate or vanillate catabolism, at 1,277,735 to 1,278,448 _1090 Pf6N2E2_1091 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 1,278,642 to 1,279,592 _1091 Pf6N2E2_1092 - Probable vanillate O-demethylase oxygenase subunit oxidoreductase protein (EC 1.14.13.-), at 1,279,589 to 1,280,674 _1092 Position (kb) 1278 1279 1280Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1277.700 kb on + strandat 1277.700 kb on + strandat 1277.701 kb on - strandat 1277.701 kb on - strandat 1277.703 kb on + strandat 1277.703 kb on + strandat 1277.703 kb on + strandat 1277.704 kb on - strandat 1277.740 kb on + strandat 1277.741 kb on - strandat 1277.823 kb on + strand, within Pf6N2E2_1090at 1278.092 kb on + strand, within Pf6N2E2_1090at 1278.092 kb on + strand, within Pf6N2E2_1090at 1278.093 kb on - strand, within Pf6N2E2_1090at 1278.281 kb on + strand, within Pf6N2E2_1090at 1278.281 kb on + strand, within Pf6N2E2_1090at 1278.281 kb on + strand, within Pf6N2E2_1090at 1278.282 kb on - strand, within Pf6N2E2_1090at 1278.289 kb on + strand, within Pf6N2E2_1090at 1278.289 kb on + strand, within Pf6N2E2_1090at 1278.289 kb on + strand, within Pf6N2E2_1090at 1278.289 kb on + strand, within Pf6N2E2_1090at 1278.468 kb on + strandat 1278.468 kb on + strandat 1278.468 kb on + strandat 1278.468 kb on + strandat 1278.469 kb on - strandat 1278.537 kb on + strandat 1278.537 kb on + strandat 1278.538 kb on - strandat 1278.538 kb on - strandat 1278.550 kb on + strandat 1278.751 kb on - strand, within Pf6N2E2_1091at 1278.751 kb on - strand, within Pf6N2E2_1091at 1278.944 kb on - strand, within Pf6N2E2_1091at 1278.944 kb on - strand, within Pf6N2E2_1091at 1279.031 kb on - strand, within Pf6N2E2_1091at 1279.183 kb on + strand, within Pf6N2E2_1091at 1279.725 kb on - strand, within Pf6N2E2_1092at 1279.872 kb on + strand, within Pf6N2E2_1092at 1279.872 kb on + strand, within Pf6N2E2_1092at 1280.199 kb on + strand, within Pf6N2E2_1092at 1280.199 kb on + strand, within Pf6N2E2_1092at 1280.200 kb on - strand, within Pf6N2E2_1092

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.002 mg/ml
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1,277,700 + -1.2
1,277,700 + +0.9
1,277,701 - +1.3
1,277,701 - -0.8
1,277,703 + -0.7
1,277,703 + +0.5
1,277,703 + +1.3
1,277,704 - -1.1
1,277,740 + +1.1
1,277,741 - -1.1
1,277,823 + Pf6N2E2_1090 0.12 -0.2
1,278,092 + Pf6N2E2_1090 0.50 +0.4
1,278,092 + Pf6N2E2_1090 0.50 +0.8
1,278,093 - Pf6N2E2_1090 0.50 -1.0
1,278,281 + Pf6N2E2_1090 0.76 +2.5
1,278,281 + Pf6N2E2_1090 0.76 +0.2
1,278,281 + Pf6N2E2_1090 0.76 +3.1
1,278,282 - Pf6N2E2_1090 0.77 +1.7
1,278,289 + Pf6N2E2_1090 0.78 +1.0
1,278,289 + Pf6N2E2_1090 0.78 -0.2
1,278,289 + Pf6N2E2_1090 0.78 -0.6
1,278,289 + Pf6N2E2_1090 0.78 -0.4
1,278,468 + -2.2
1,278,468 + -0.0
1,278,468 + +0.0
1,278,468 + +0.2
1,278,469 - +2.4
1,278,537 + +1.0
1,278,537 + +0.5
1,278,538 - +1.1
1,278,538 - +0.0
1,278,550 + +0.8
1,278,751 - Pf6N2E2_1091 0.11 +1.6
1,278,751 - Pf6N2E2_1091 0.11 +0.2
1,278,944 - Pf6N2E2_1091 0.32 -0.8
1,278,944 - Pf6N2E2_1091 0.32 -1.4
1,279,031 - Pf6N2E2_1091 0.41 -1.4
1,279,183 + Pf6N2E2_1091 0.57 -1.3
1,279,725 - Pf6N2E2_1092 0.13 +0.8
1,279,872 + Pf6N2E2_1092 0.26 -0.7
1,279,872 + Pf6N2E2_1092 0.26 -2.9
1,280,199 + Pf6N2E2_1092 0.56 +0.3
1,280,199 + Pf6N2E2_1092 0.56 +0.9
1,280,200 - Pf6N2E2_1092 0.56 +0.1

Or see this region's nucleotide sequence