Experiment: A22 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1090 and Pf6N2E2_1091 are separated by 193 nucleotides Pf6N2E2_1091 and Pf6N2E2_1092 overlap by 4 nucleotides
Pf6N2E2_1090 - Transcriptional regulator for ferulate or vanillate catabolism, at 1,277,735 to 1,278,448
_1090
Pf6N2E2_1091 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 1,278,642 to 1,279,592
_1091
Pf6N2E2_1092 - Probable vanillate O-demethylase oxygenase subunit oxidoreductase protein (EC 1.14.13.-), at 1,279,589 to 1,280,674
_1092
Position (kb)
1278
1279
1280 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1277.700 kb on + strand at 1277.700 kb on + strand at 1277.701 kb on - strand at 1277.701 kb on - strand at 1277.703 kb on + strand at 1277.703 kb on + strand at 1277.703 kb on + strand at 1277.704 kb on - strand at 1277.740 kb on + strand at 1277.741 kb on - strand at 1277.823 kb on + strand, within Pf6N2E2_1090 at 1278.092 kb on + strand, within Pf6N2E2_1090 at 1278.092 kb on + strand, within Pf6N2E2_1090 at 1278.093 kb on - strand, within Pf6N2E2_1090 at 1278.281 kb on + strand, within Pf6N2E2_1090 at 1278.281 kb on + strand, within Pf6N2E2_1090 at 1278.281 kb on + strand, within Pf6N2E2_1090 at 1278.282 kb on - strand, within Pf6N2E2_1090 at 1278.289 kb on + strand, within Pf6N2E2_1090 at 1278.289 kb on + strand, within Pf6N2E2_1090 at 1278.289 kb on + strand, within Pf6N2E2_1090 at 1278.289 kb on + strand, within Pf6N2E2_1090 at 1278.468 kb on + strand at 1278.468 kb on + strand at 1278.468 kb on + strand at 1278.468 kb on + strand at 1278.469 kb on - strand at 1278.537 kb on + strand at 1278.537 kb on + strand at 1278.538 kb on - strand at 1278.538 kb on - strand at 1278.550 kb on + strand at 1278.751 kb on - strand, within Pf6N2E2_1091 at 1278.751 kb on - strand, within Pf6N2E2_1091 at 1278.944 kb on - strand, within Pf6N2E2_1091 at 1278.944 kb on - strand, within Pf6N2E2_1091 at 1279.031 kb on - strand, within Pf6N2E2_1091 at 1279.183 kb on + strand, within Pf6N2E2_1091 at 1279.725 kb on - strand, within Pf6N2E2_1092 at 1279.872 kb on + strand, within Pf6N2E2_1092 at 1279.872 kb on + strand, within Pf6N2E2_1092 at 1280.199 kb on + strand, within Pf6N2E2_1092 at 1280.199 kb on + strand, within Pf6N2E2_1092 at 1280.200 kb on - strand, within Pf6N2E2_1092
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.002 mg/ml remove 1,277,700 + -1.2 1,277,700 + +0.9 1,277,701 - +1.3 1,277,701 - -0.8 1,277,703 + -0.7 1,277,703 + +0.5 1,277,703 + +1.3 1,277,704 - -1.1 1,277,740 + +1.1 1,277,741 - -1.1 1,277,823 + Pf6N2E2_1090 0.12 -0.2 1,278,092 + Pf6N2E2_1090 0.50 +0.4 1,278,092 + Pf6N2E2_1090 0.50 +0.8 1,278,093 - Pf6N2E2_1090 0.50 -1.0 1,278,281 + Pf6N2E2_1090 0.76 +2.5 1,278,281 + Pf6N2E2_1090 0.76 +0.2 1,278,281 + Pf6N2E2_1090 0.76 +3.1 1,278,282 - Pf6N2E2_1090 0.77 +1.7 1,278,289 + Pf6N2E2_1090 0.78 +1.0 1,278,289 + Pf6N2E2_1090 0.78 -0.2 1,278,289 + Pf6N2E2_1090 0.78 -0.6 1,278,289 + Pf6N2E2_1090 0.78 -0.4 1,278,468 + -2.2 1,278,468 + -0.0 1,278,468 + +0.0 1,278,468 + +0.2 1,278,469 - +2.4 1,278,537 + +1.0 1,278,537 + +0.5 1,278,538 - +1.1 1,278,538 - +0.0 1,278,550 + +0.8 1,278,751 - Pf6N2E2_1091 0.11 +1.6 1,278,751 - Pf6N2E2_1091 0.11 +0.2 1,278,944 - Pf6N2E2_1091 0.32 -0.8 1,278,944 - Pf6N2E2_1091 0.32 -1.4 1,279,031 - Pf6N2E2_1091 0.41 -1.4 1,279,183 + Pf6N2E2_1091 0.57 -1.3 1,279,725 - Pf6N2E2_1092 0.13 +0.8 1,279,872 + Pf6N2E2_1092 0.26 -0.7 1,279,872 + Pf6N2E2_1092 0.26 -2.9 1,280,199 + Pf6N2E2_1092 0.56 +0.3 1,280,199 + Pf6N2E2_1092 0.56 +0.9 1,280,200 - Pf6N2E2_1092 0.56 +0.1
Or see this region's nucleotide sequence