Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06523 and H281DRAFT_06524 overlap by 4 nucleotides H281DRAFT_06524 and H281DRAFT_06525 are separated by 6 nucleotides
H281DRAFT_06522: H281DRAFT_06522 - hypothetical protein, at 1,983 to 2,420
_06522
H281DRAFT_06523: H281DRAFT_06523 - protoporphyrin IX magnesium-chelatase (EC 6.6.1.1), at 2,228 to 3,310
_06523
H281DRAFT_06524: H281DRAFT_06524 - cobaltochelatase CobN subunit, at 3,307 to 7,122
_06524
H281DRAFT_06525: H281DRAFT_06525 - cobalamin biosynthesis protein CobW, at 7,129 to 8,310
_06525
Position (kb) 3
4
5
6
7
8 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 2.344 kb on + strand at 2.410 kb on + strand at 2.564 kb on - strand, within H281DRAFT_06523 at 2.649 kb on + strand, within H281DRAFT_06523 at 2.703 kb on + strand, within H281DRAFT_06523 at 2.857 kb on + strand, within H281DRAFT_06523 at 2.857 kb on + strand, within H281DRAFT_06523 at 2.958 kb on + strand, within H281DRAFT_06523 at 2.958 kb on + strand, within H281DRAFT_06523 at 2.958 kb on + strand, within H281DRAFT_06523 at 2.958 kb on + strand, within H281DRAFT_06523 at 2.959 kb on - strand, within H281DRAFT_06523 at 2.959 kb on - strand, within H281DRAFT_06523 at 3.491 kb on + strand at 3.491 kb on + strand at 3.492 kb on - strand at 3.548 kb on + strand at 3.554 kb on + strand at 3.555 kb on - strand at 3.560 kb on + strand at 3.594 kb on + strand at 3.596 kb on + strand at 3.597 kb on - strand at 3.611 kb on - strand at 3.720 kb on - strand, within H281DRAFT_06524 at 3.786 kb on - strand, within H281DRAFT_06524 at 3.835 kb on + strand, within H281DRAFT_06524 at 3.835 kb on + strand, within H281DRAFT_06524 at 3.905 kb on + strand, within H281DRAFT_06524 at 3.906 kb on - strand, within H281DRAFT_06524 at 3.906 kb on - strand, within H281DRAFT_06524 at 3.929 kb on + strand, within H281DRAFT_06524 at 3.930 kb on - strand, within H281DRAFT_06524 at 4.387 kb on + strand, within H281DRAFT_06524 at 4.713 kb on + strand, within H281DRAFT_06524 at 4.713 kb on + strand, within H281DRAFT_06524 at 4.714 kb on - strand, within H281DRAFT_06524 at 4.719 kb on + strand, within H281DRAFT_06524 at 5.092 kb on + strand, within H281DRAFT_06524 at 5.151 kb on + strand, within H281DRAFT_06524 at 5.152 kb on - strand, within H281DRAFT_06524 at 5.152 kb on - strand, within H281DRAFT_06524 at 5.152 kb on - strand, within H281DRAFT_06524 at 5.163 kb on + strand, within H281DRAFT_06524 at 5.185 kb on + strand, within H281DRAFT_06524 at 5.225 kb on + strand, within H281DRAFT_06524 at 5.226 kb on - strand, within H281DRAFT_06524 at 5.226 kb on - strand, within H281DRAFT_06524 at 5.228 kb on + strand, within H281DRAFT_06524 at 5.385 kb on + strand, within H281DRAFT_06524 at 5.416 kb on - strand, within H281DRAFT_06524 at 5.416 kb on - strand, within H281DRAFT_06524 at 5.516 kb on + strand, within H281DRAFT_06524 at 5.517 kb on - strand, within H281DRAFT_06524 at 5.528 kb on + strand, within H281DRAFT_06524 at 5.562 kb on - strand, within H281DRAFT_06524 at 5.615 kb on - strand, within H281DRAFT_06524 at 5.745 kb on - strand, within H281DRAFT_06524 at 5.745 kb on - strand, within H281DRAFT_06524 at 5.831 kb on - strand, within H281DRAFT_06524 at 5.961 kb on - strand, within H281DRAFT_06524 at 6.171 kb on + strand, within H281DRAFT_06524 at 6.196 kb on - strand, within H281DRAFT_06524 at 6.368 kb on - strand, within H281DRAFT_06524 at 6.368 kb on - strand, within H281DRAFT_06524 at 6.403 kb on - strand, within H281DRAFT_06524 at 6.845 kb on + strand at 6.846 kb on - strand at 6.943 kb on + strand at 6.943 kb on + strand at 7.080 kb on + strand at 7.081 kb on - strand at 7.284 kb on - strand, within H281DRAFT_06525 at 7.284 kb on - strand, within H281DRAFT_06525 at 7.405 kb on + strand, within H281DRAFT_06525 at 7.548 kb on + strand, within H281DRAFT_06525 at 8.015 kb on + strand, within H281DRAFT_06525 at 8.070 kb on + strand, within H281DRAFT_06525
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM remove 2,344 + +0.1 2,410 + -1.9 2,564 - H281DRAFT_06523 0.31 -2.5 2,649 + H281DRAFT_06523 0.39 -3.1 2,703 + H281DRAFT_06523 0.44 -2.5 2,857 + H281DRAFT_06523 0.58 -0.6 2,857 + H281DRAFT_06523 0.58 -2.5 2,958 + H281DRAFT_06523 0.67 -3.7 2,958 + H281DRAFT_06523 0.67 -1.5 2,958 + H281DRAFT_06523 0.67 -0.5 2,958 + H281DRAFT_06523 0.67 -2.0 2,959 - H281DRAFT_06523 0.67 +0.4 2,959 - H281DRAFT_06523 0.67 -1.3 3,491 + -3.9 3,491 + -1.9 3,492 - -0.5 3,548 + -0.5 3,554 + -1.1 3,555 - -0.8 3,560 + -1.9 3,594 + -2.0 3,596 + -0.5 3,597 - -1.5 3,611 - -2.4 3,720 - H281DRAFT_06524 0.11 -3.8 3,786 - H281DRAFT_06524 0.13 -0.6 3,835 + H281DRAFT_06524 0.14 +0.4 3,835 + H281DRAFT_06524 0.14 -0.2 3,905 + H281DRAFT_06524 0.16 -3.4 3,906 - H281DRAFT_06524 0.16 -4.6 3,906 - H281DRAFT_06524 0.16 +0.9 3,929 + H281DRAFT_06524 0.16 -0.5 3,930 - H281DRAFT_06524 0.16 -2.2 4,387 + H281DRAFT_06524 0.28 -2.4 4,713 + H281DRAFT_06524 0.37 -3.1 4,713 + H281DRAFT_06524 0.37 -0.7 4,714 - H281DRAFT_06524 0.37 -3.6 4,719 + H281DRAFT_06524 0.37 -0.6 5,092 + H281DRAFT_06524 0.47 -1.2 5,151 + H281DRAFT_06524 0.48 -1.8 5,152 - H281DRAFT_06524 0.48 -0.5 5,152 - H281DRAFT_06524 0.48 -2.0 5,152 - H281DRAFT_06524 0.48 -1.7 5,163 + H281DRAFT_06524 0.49 -1.1 5,185 + H281DRAFT_06524 0.49 -1.9 5,225 + H281DRAFT_06524 0.50 -3.1 5,226 - H281DRAFT_06524 0.50 -3.1 5,226 - H281DRAFT_06524 0.50 +0.3 5,228 + H281DRAFT_06524 0.50 -1.3 5,385 + H281DRAFT_06524 0.54 -1.5 5,416 - H281DRAFT_06524 0.55 -0.0 5,416 - H281DRAFT_06524 0.55 -2.1 5,516 + H281DRAFT_06524 0.58 -1.4 5,517 - H281DRAFT_06524 0.58 -2.5 5,528 + H281DRAFT_06524 0.58 -2.3 5,562 - H281DRAFT_06524 0.59 -1.1 5,615 - H281DRAFT_06524 0.60 -0.2 5,745 - H281DRAFT_06524 0.64 -2.1 5,745 - H281DRAFT_06524 0.64 +0.1 5,831 - H281DRAFT_06524 0.66 -2.6 5,961 - H281DRAFT_06524 0.70 -2.1 6,171 + H281DRAFT_06524 0.75 +0.1 6,196 - H281DRAFT_06524 0.76 -1.2 6,368 - H281DRAFT_06524 0.80 -2.0 6,368 - H281DRAFT_06524 0.80 -0.4 6,403 - H281DRAFT_06524 0.81 -3.0 6,845 + -3.1 6,846 - +0.1 6,943 + -2.6 6,943 + -0.9 7,080 + +0.3 7,081 - -1.9 7,284 - H281DRAFT_06525 0.13 -0.2 7,284 - H281DRAFT_06525 0.13 +0.1 7,405 + H281DRAFT_06525 0.23 -2.2 7,548 + H281DRAFT_06525 0.35 -1.5 8,015 + H281DRAFT_06525 0.75 -0.9 8,070 + H281DRAFT_06525 0.80 -0.7
Or see this region's nucleotide sequence