Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05427

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05425 and H281DRAFT_05426 are separated by 117 nucleotidesH281DRAFT_05426 and H281DRAFT_05427 overlap by 24 nucleotidesH281DRAFT_05427 and H281DRAFT_05428 overlap by 4 nucleotides H281DRAFT_05425: H281DRAFT_05425 - Transposase, at 1,161 to 2,231 _05425 H281DRAFT_05426: H281DRAFT_05426 - hypothetical protein, at 2,349 to 2,900 _05426 H281DRAFT_05427: H281DRAFT_05427 - Two-component response regulator, AmiR/NasT family, consists of REC and RNA-binding antiterminator (ANTAR) domains, at 2,877 to 3,497 _05427 H281DRAFT_05428: H281DRAFT_05428 - amino acid/amide ABC transporter substrate-binding protein, HAAT family, at 3,494 to 4,669 _05428 Position (kb) 2 3 4Strain fitness (log2 ratio) -2 -1 0 1at 1.900 kb on + strand, within H281DRAFT_05425at 2.228 kb on - strandat 2.228 kb on - strandat 2.252 kb on + strandat 2.394 kb on + strandat 2.394 kb on + strandat 2.394 kb on + strandat 2.395 kb on - strandat 2.475 kb on - strand, within H281DRAFT_05426at 2.503 kb on - strand, within H281DRAFT_05426at 2.503 kb on - strand, within H281DRAFT_05426at 2.503 kb on - strand, within H281DRAFT_05426at 2.503 kb on - strand, within H281DRAFT_05426at 2.503 kb on - strand, within H281DRAFT_05426at 2.504 kb on + strand, within H281DRAFT_05426at 2.504 kb on + strand, within H281DRAFT_05426at 2.504 kb on + strand, within H281DRAFT_05426at 2.504 kb on + strand, within H281DRAFT_05426at 2.504 kb on + strand, within H281DRAFT_05426at 2.504 kb on + strand, within H281DRAFT_05426at 2.505 kb on - strand, within H281DRAFT_05426at 2.505 kb on - strand, within H281DRAFT_05426at 2.505 kb on - strand, within H281DRAFT_05426at 2.505 kb on - strand, within H281DRAFT_05426at 2.505 kb on - strand, within H281DRAFT_05426at 2.655 kb on - strand, within H281DRAFT_05426at 2.661 kb on - strand, within H281DRAFT_05426at 2.694 kb on + strand, within H281DRAFT_05426at 2.694 kb on + strand, within H281DRAFT_05426at 2.849 kb on + strandat 2.889 kb on + strandat 2.956 kb on - strand, within H281DRAFT_05427at 2.998 kb on + strand, within H281DRAFT_05427at 2.998 kb on + strand, within H281DRAFT_05427at 3.375 kb on + strand, within H281DRAFT_05427at 3.375 kb on + strand, within H281DRAFT_05427at 3.376 kb on - strand, within H281DRAFT_05427at 3.534 kb on + strandat 3.764 kb on + strand, within H281DRAFT_05428at 3.774 kb on + strand, within H281DRAFT_05428at 3.774 kb on + strand, within H281DRAFT_05428at 3.775 kb on - strand, within H281DRAFT_05428at 3.873 kb on + strand, within H281DRAFT_05428at 3.873 kb on + strand, within H281DRAFT_05428at 3.873 kb on + strand, within H281DRAFT_05428at 3.874 kb on - strand, within H281DRAFT_05428at 3.874 kb on - strand, within H281DRAFT_05428at 3.990 kb on + strand, within H281DRAFT_05428at 3.991 kb on - strand, within H281DRAFT_05428at 4.114 kb on - strand, within H281DRAFT_05428at 4.186 kb on - strand, within H281DRAFT_05428at 4.186 kb on - strand, within H281DRAFT_05428at 4.191 kb on + strand, within H281DRAFT_05428at 4.206 kb on - strand, within H281DRAFT_05428at 4.239 kb on + strand, within H281DRAFT_05428at 4.284 kb on + strand, within H281DRAFT_05428at 4.285 kb on - strand, within H281DRAFT_05428at 4.302 kb on + strand, within H281DRAFT_05428at 4.317 kb on + strand, within H281DRAFT_05428at 4.329 kb on + strand, within H281DRAFT_05428at 4.330 kb on - strand, within H281DRAFT_05428at 4.480 kb on - strand, within H281DRAFT_05428

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
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1,900 + H281DRAFT_05425 0.69 +1.5
2,228 - +0.2
2,228 - +0.5
2,252 + +0.5
2,394 + +0.1
2,394 + -0.2
2,394 + +0.2
2,395 - -0.1
2,475 - H281DRAFT_05426 0.23 +1.7
2,503 - H281DRAFT_05426 0.28 +0.6
2,503 - H281DRAFT_05426 0.28 -0.1
2,503 - H281DRAFT_05426 0.28 +0.0
2,503 - H281DRAFT_05426 0.28 -2.7
2,503 - H281DRAFT_05426 0.28 -1.7
2,504 + H281DRAFT_05426 0.28 +1.1
2,504 + H281DRAFT_05426 0.28 -0.2
2,504 + H281DRAFT_05426 0.28 -0.1
2,504 + H281DRAFT_05426 0.28 +1.4
2,504 + H281DRAFT_05426 0.28 -0.7
2,504 + H281DRAFT_05426 0.28 -0.4
2,505 - H281DRAFT_05426 0.28 -0.8
2,505 - H281DRAFT_05426 0.28 -0.3
2,505 - H281DRAFT_05426 0.28 +0.3
2,505 - H281DRAFT_05426 0.28 -0.0
2,505 - H281DRAFT_05426 0.28 +0.4
2,655 - H281DRAFT_05426 0.55 -0.9
2,661 - H281DRAFT_05426 0.57 +0.1
2,694 + H281DRAFT_05426 0.62 -0.0
2,694 + H281DRAFT_05426 0.62 -0.2
2,849 + -1.3
2,889 + -0.8
2,956 - H281DRAFT_05427 0.13 -0.5
2,998 + H281DRAFT_05427 0.19 -1.0
2,998 + H281DRAFT_05427 0.19 +0.3
3,375 + H281DRAFT_05427 0.80 -0.3
3,375 + H281DRAFT_05427 0.80 -0.3
3,376 - H281DRAFT_05427 0.80 +0.0
3,534 + +0.3
3,764 + H281DRAFT_05428 0.23 +0.1
3,774 + H281DRAFT_05428 0.24 +0.3
3,774 + H281DRAFT_05428 0.24 +1.0
3,775 - H281DRAFT_05428 0.24 +0.5
3,873 + H281DRAFT_05428 0.32 -0.0
3,873 + H281DRAFT_05428 0.32 -0.4
3,873 + H281DRAFT_05428 0.32 -0.4
3,874 - H281DRAFT_05428 0.32 +0.4
3,874 - H281DRAFT_05428 0.32 +0.8
3,990 + H281DRAFT_05428 0.42 +0.5
3,991 - H281DRAFT_05428 0.42 +0.2
4,114 - H281DRAFT_05428 0.53 -0.4
4,186 - H281DRAFT_05428 0.59 +0.5
4,186 - H281DRAFT_05428 0.59 -0.0
4,191 + H281DRAFT_05428 0.59 -0.1
4,206 - H281DRAFT_05428 0.61 +0.8
4,239 + H281DRAFT_05428 0.63 -0.3
4,284 + H281DRAFT_05428 0.67 +0.2
4,285 - H281DRAFT_05428 0.67 -0.1
4,302 + H281DRAFT_05428 0.69 -0.0
4,317 + H281DRAFT_05428 0.70 -0.1
4,329 + H281DRAFT_05428 0.71 +1.2
4,330 - H281DRAFT_05428 0.71 +0.9
4,480 - H281DRAFT_05428 0.84 -1.6

Or see this region's nucleotide sequence