Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05299

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05297 and H281DRAFT_05298 are separated by 18 nucleotidesH281DRAFT_05298 and H281DRAFT_05299 are separated by 19 nucleotidesH281DRAFT_05299 and H281DRAFT_05300 are separated by 90 nucleotides H281DRAFT_05297: H281DRAFT_05297 - amino acid ABC transporter ATP-binding protein, PAAT family, at 40,184 to 41,047 _05297 H281DRAFT_05298: H281DRAFT_05298 - amino acid ABC transporter membrane protein 2, PAAT family, at 41,066 to 41,728 _05298 H281DRAFT_05299: H281DRAFT_05299 - amino acid ABC transporter membrane protein 1, PAAT family, at 41,748 to 42,398 _05299 H281DRAFT_05300: H281DRAFT_05300 - amino acid ABC transporter substrate-binding protein, PAAT family, at 42,489 to 43,316 _05300 Position (kb) 41 42 43Strain fitness (log2 ratio) -2 -1 0 1 2at 40.785 kb on + strand, within H281DRAFT_05297at 40.896 kb on - strand, within H281DRAFT_05297at 41.553 kb on + strand, within H281DRAFT_05298at 41.728 kb on + strandat 41.729 kb on - strandat 41.816 kb on - strand, within H281DRAFT_05299at 41.854 kb on + strandat 41.854 kb on + strand, within H281DRAFT_05299at 41.855 kb on - strand, within H281DRAFT_05299at 42.046 kb on - strand, within H281DRAFT_05299at 42.046 kb on - strand, within H281DRAFT_05299at 42.046 kb on - strand, within H281DRAFT_05299at 42.097 kb on + strand, within H281DRAFT_05299at 42.097 kb on + strand, within H281DRAFT_05299at 42.098 kb on - strand, within H281DRAFT_05299at 42.176 kb on - strand, within H281DRAFT_05299at 42.229 kb on + strand, within H281DRAFT_05299at 42.468 kb on + strandat 42.719 kb on - strand, within H281DRAFT_05300at 42.839 kb on - strand, within H281DRAFT_05300at 42.839 kb on - strand, within H281DRAFT_05300at 42.976 kb on + strand, within H281DRAFT_05300at 42.977 kb on - strand, within H281DRAFT_05300at 43.382 kb on - strandat 43.383 kb on + strandat 43.383 kb on + strandat 43.384 kb on - strandat 43.385 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
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40,785 + H281DRAFT_05297 0.70 -0.0
40,896 - H281DRAFT_05297 0.82 -0.3
41,553 + H281DRAFT_05298 0.73 -1.3
41,728 + +0.8
41,729 - +0.8
41,816 - H281DRAFT_05299 0.10 -0.5
41,854 + -1.8
41,854 + H281DRAFT_05299 0.16 +0.5
41,855 - H281DRAFT_05299 0.16 -0.8
42,046 - H281DRAFT_05299 0.46 +0.2
42,046 - H281DRAFT_05299 0.46 +1.0
42,046 - H281DRAFT_05299 0.46 +0.7
42,097 + H281DRAFT_05299 0.54 -0.1
42,097 + H281DRAFT_05299 0.54 +0.4
42,098 - H281DRAFT_05299 0.54 +0.6
42,176 - H281DRAFT_05299 0.66 +0.5
42,229 + H281DRAFT_05299 0.74 +1.0
42,468 + -0.6
42,719 - H281DRAFT_05300 0.28 +0.5
42,839 - H281DRAFT_05300 0.42 +2.4
42,839 - H281DRAFT_05300 0.42 +0.6
42,976 + H281DRAFT_05300 0.59 +1.0
42,977 - H281DRAFT_05300 0.59 +1.1
43,382 - -0.0
43,383 + -0.5
43,383 + -0.6
43,384 - +0.2
43,385 + -0.6

Or see this region's nucleotide sequence