Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_04552

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_04551 and H281DRAFT_04552 are separated by 111 nucleotidesH281DRAFT_04552 and H281DRAFT_04553 are separated by 112 nucleotides H281DRAFT_04551: H281DRAFT_04551 - acetolactate synthase, large subunit, at 282,479 to 284,242 _04551 H281DRAFT_04552: H281DRAFT_04552 - acetolactate synthase, small subunit, at 284,354 to 284,845 _04552 H281DRAFT_04553: H281DRAFT_04553 - ketol-acid reductoisomerase, at 284,958 to 285,974 _04553 Position (kb) 284 285Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 284.238 kb on + strandat 284.238 kb on + strandat 284.323 kb on + strandat 284.324 kb on - strandat 284.365 kb on + strandat 284.711 kb on + strand, within H281DRAFT_04552at 284.711 kb on + strand, within H281DRAFT_04552at 284.711 kb on + strand, within H281DRAFT_04552at 284.711 kb on + strand, within H281DRAFT_04552at 284.914 kb on - strandat 285.223 kb on - strand, within H281DRAFT_04553at 285.375 kb on + strand, within H281DRAFT_04553at 285.375 kb on + strand, within H281DRAFT_04553at 285.376 kb on - strand, within H281DRAFT_04553at 285.376 kb on - strand, within H281DRAFT_04553at 285.672 kb on + strand, within H281DRAFT_04553at 285.672 kb on + strand, within H281DRAFT_04553at 285.673 kb on - strand, within H281DRAFT_04553at 285.673 kb on - strand, within H281DRAFT_04553at 285.732 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
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284,238 + -1.9
284,238 + +0.4
284,323 + +1.0
284,324 - +1.0
284,365 + -1.0
284,711 + H281DRAFT_04552 0.73 +0.4
284,711 + H281DRAFT_04552 0.73 +0.0
284,711 + H281DRAFT_04552 0.73 -0.3
284,711 + H281DRAFT_04552 0.73 -0.1
284,914 - -1.9
285,223 - H281DRAFT_04553 0.26 -3.4
285,375 + H281DRAFT_04553 0.41 -2.4
285,375 + H281DRAFT_04553 0.41 -3.8
285,376 - H281DRAFT_04553 0.41 -2.1
285,376 - H281DRAFT_04553 0.41 -2.4
285,672 + H281DRAFT_04553 0.70 -3.5
285,672 + H281DRAFT_04553 0.70 -3.9
285,673 - H281DRAFT_04553 0.70 -0.9
285,673 - H281DRAFT_04553 0.70 -3.5
285,732 + +0.9

Or see this region's nucleotide sequence