Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_00009

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_00007 and H281DRAFT_00008 overlap by 4 nucleotidesH281DRAFT_00008 and H281DRAFT_00009 overlap by 4 nucleotidesH281DRAFT_00009 and H281DRAFT_00010 are separated by 300 nucleotidesH281DRAFT_00010 and H281DRAFT_00011 overlap by 4 nucleotides H281DRAFT_00007: H281DRAFT_00007 - Site-specific DNA recombinase, at 6,666 to 8,261 _00007 H281DRAFT_00008: H281DRAFT_00008 - hypothetical protein, at 8,258 to 8,575 _00008 H281DRAFT_00009: H281DRAFT_00009 - RepB plasmid partitioning protein, at 8,572 to 9,186 _00009 H281DRAFT_00010: H281DRAFT_00010 - nitrile hydratase, at 9,487 to 10,146 _00010 H281DRAFT_00011: H281DRAFT_00011 - nitrile hydratase, at 10,143 to 10,814 _00011 Position (kb) 8 9 10Strain fitness (log2 ratio) -2 -1 0 1at 7.666 kb on + strand, within H281DRAFT_00007at 7.767 kb on + strand, within H281DRAFT_00007at 7.767 kb on + strand, within H281DRAFT_00007at 7.768 kb on - strand, within H281DRAFT_00007at 8.119 kb on - strandat 8.120 kb on + strandat 8.121 kb on - strandat 8.121 kb on - strandat 8.160 kb on + strandat 8.467 kb on - strand, within H281DRAFT_00008at 8.799 kb on - strand, within H281DRAFT_00009at 8.799 kb on - strand, within H281DRAFT_00009at 8.938 kb on + strand, within H281DRAFT_00009at 8.954 kb on - strand, within H281DRAFT_00009at 8.954 kb on - strand, within H281DRAFT_00009at 8.957 kb on - strand, within H281DRAFT_00009at 8.957 kb on - strand, within H281DRAFT_00009at 9.020 kb on + strand, within H281DRAFT_00009at 9.115 kb on + strand, within H281DRAFT_00009at 9.220 kb on + strandat 9.221 kb on - strandat 9.238 kb on + strandat 9.268 kb on + strandat 9.269 kb on - strandat 9.310 kb on - strandat 9.450 kb on + strandat 9.450 kb on + strandat 9.608 kb on - strand, within H281DRAFT_00010at 9.647 kb on - strand, within H281DRAFT_00010at 9.686 kb on + strand, within H281DRAFT_00010at 9.713 kb on - strand, within H281DRAFT_00010at 9.834 kb on + strand, within H281DRAFT_00010at 9.935 kb on + strand, within H281DRAFT_00010at 10.142 kb on + strandat 10.142 kb on + strandat 10.142 kb on + strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strandat 10.143 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
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7,666 + H281DRAFT_00007 0.63 +0.1
7,767 + H281DRAFT_00007 0.69 +0.6
7,767 + H281DRAFT_00007 0.69 -0.2
7,768 - H281DRAFT_00007 0.69 +0.0
8,119 - +0.7
8,120 + -0.4
8,121 - -1.1
8,121 - +0.0
8,160 + +0.1
8,467 - H281DRAFT_00008 0.66 -0.8
8,799 - H281DRAFT_00009 0.37 +0.3
8,799 - H281DRAFT_00009 0.37 -1.0
8,938 + H281DRAFT_00009 0.60 +0.1
8,954 - H281DRAFT_00009 0.62 -0.9
8,954 - H281DRAFT_00009 0.62 +0.4
8,957 - H281DRAFT_00009 0.63 +0.6
8,957 - H281DRAFT_00009 0.63 +0.9
9,020 + H281DRAFT_00009 0.73 +0.3
9,115 + H281DRAFT_00009 0.88 +0.3
9,220 + +0.8
9,221 - -0.1
9,238 + +0.7
9,268 + -1.9
9,269 - +0.3
9,310 - +0.0
9,450 + -0.5
9,450 + +0.4
9,608 - H281DRAFT_00010 0.18 -1.0
9,647 - H281DRAFT_00010 0.24 -0.5
9,686 + H281DRAFT_00010 0.30 -0.3
9,713 - H281DRAFT_00010 0.34 -1.7
9,834 + H281DRAFT_00010 0.53 +0.6
9,935 + H281DRAFT_00010 0.68 -0.1
10,142 + -0.3
10,142 + +1.2
10,142 + +0.1
10,143 - +0.4
10,143 - +0.2
10,143 - +1.3
10,143 - -0.5
10,143 - -0.3
10,143 - -0.4
10,143 - +0.8

Or see this region's nucleotide sequence