Experiment: Fusidic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3839 and Ac3H11_3840 are separated by 50 nucleotides Ac3H11_3840 and Ac3H11_3841 are separated by 73 nucleotides Ac3H11_3841 and Ac3H11_3842 are separated by 150 nucleotides
Ac3H11_3839 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 55,742 to 56,623
_3839
Ac3H11_3840 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 56,674 to 57,519
_3840
Ac3H11_3841 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 57,593 to 58,315
_3841
Ac3H11_3842 - Large protein containing transglutaminase-like domain, at 58,466 to 61,963
_3842
Position (kb)
57
58
59 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 56.873 kb on - strand, within Ac3H11_3840 at 56.956 kb on + strand, within Ac3H11_3840 at 57.124 kb on + strand, within Ac3H11_3840 at 57.124 kb on + strand, within Ac3H11_3840 at 57.242 kb on - strand, within Ac3H11_3840 at 57.394 kb on + strand, within Ac3H11_3840 at 57.395 kb on - strand, within Ac3H11_3840 at 57.550 kb on + strand at 57.886 kb on - strand, within Ac3H11_3841 at 57.886 kb on - strand, within Ac3H11_3841 at 58.296 kb on - strand at 58.315 kb on + strand at 58.316 kb on - strand at 58.451 kb on + strand at 58.451 kb on + strand at 58.451 kb on + strand at 58.451 kb on + strand at 58.452 kb on - strand at 58.452 kb on - strand at 58.465 kb on + strand at 58.465 kb on + strand at 58.465 kb on + strand at 58.465 kb on + strand at 58.465 kb on + strand at 58.466 kb on - strand at 58.466 kb on - strand at 58.466 kb on - strand at 58.502 kb on + strand at 58.502 kb on + strand at 58.502 kb on + strand at 58.503 kb on - strand at 58.503 kb on - strand at 58.649 kb on + strand at 58.649 kb on + strand at 58.650 kb on - strand at 58.650 kb on - strand at 58.650 kb on - strand at 58.650 kb on - strand at 58.728 kb on - strand at 58.728 kb on - strand at 58.781 kb on + strand at 58.782 kb on - strand at 58.782 kb on - strand at 58.782 kb on - strand at 58.782 kb on - strand at 58.814 kb on + strand at 58.814 kb on + strand at 58.815 kb on - strand at 58.815 kb on - strand at 58.815 kb on - strand at 58.815 kb on - strand at 58.853 kb on + strand, within Ac3H11_3842 at 58.854 kb on - strand, within Ac3H11_3842 at 59.034 kb on + strand, within Ac3H11_3842 at 59.035 kb on - strand, within Ac3H11_3842 at 59.035 kb on - strand, within Ac3H11_3842 at 59.035 kb on - strand, within Ac3H11_3842 at 59.168 kb on + strand, within Ac3H11_3842 at 59.168 kb on + strand, within Ac3H11_3842 at 59.168 kb on + strand, within Ac3H11_3842 at 59.168 kb on + strand, within Ac3H11_3842 at 59.169 kb on - strand, within Ac3H11_3842 at 59.169 kb on - strand, within Ac3H11_3842 at 59.169 kb on - strand, within Ac3H11_3842
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.001 mg/ml remove 56,873 - Ac3H11_3840 0.24 -1.3 56,956 + Ac3H11_3840 0.33 +0.4 57,124 + Ac3H11_3840 0.53 +0.7 57,124 + Ac3H11_3840 0.53 +0.3 57,242 - Ac3H11_3840 0.67 +1.1 57,394 + Ac3H11_3840 0.85 +0.9 57,395 - Ac3H11_3840 0.85 +0.8 57,550 + +0.6 57,886 - Ac3H11_3841 0.41 -1.5 57,886 - Ac3H11_3841 0.41 -0.3 58,296 - +0.5 58,315 + +0.9 58,316 - +0.2 58,451 + -0.1 58,451 + +0.3 58,451 + -0.8 58,451 + -0.1 58,452 - -1.1 58,452 - -1.1 58,465 + -0.5 58,465 + -0.6 58,465 + +1.2 58,465 + +1.9 58,465 + +0.5 58,466 - +0.3 58,466 - +1.3 58,466 - -0.8 58,502 + -0.2 58,502 + -0.4 58,502 + +0.7 58,503 - -0.4 58,503 - -0.9 58,649 + +0.2 58,649 + -0.8 58,650 - -1.4 58,650 - +0.1 58,650 - +0.4 58,650 - +0.1 58,728 - +1.1 58,728 - +1.1 58,781 + +0.3 58,782 - +1.5 58,782 - -0.3 58,782 - +0.1 58,782 - -0.1 58,814 + -1.0 58,814 + -1.1 58,815 - -3.4 58,815 - +0.4 58,815 - -0.7 58,815 - +1.3 58,853 + Ac3H11_3842 0.11 -0.2 58,854 - Ac3H11_3842 0.11 -0.6 59,034 + Ac3H11_3842 0.16 -0.3 59,035 - Ac3H11_3842 0.16 -0.7 59,035 - Ac3H11_3842 0.16 -0.7 59,035 - Ac3H11_3842 0.16 -1.2 59,168 + Ac3H11_3842 0.20 +0.4 59,168 + Ac3H11_3842 0.20 -0.5 59,168 + Ac3H11_3842 0.20 +0.3 59,168 + Ac3H11_3842 0.20 -0.6 59,169 - Ac3H11_3842 0.20 +0.2 59,169 - Ac3H11_3842 0.20 +1.5 59,169 - Ac3H11_3842 0.20 -1.7
Or see this region's nucleotide sequence