Experiment: Fusidic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1818 and Ac3H11_1819 are separated by 29 nucleotides Ac3H11_1819 and Ac3H11_1820 are separated by 15 nucleotides Ac3H11_1820 and Ac3H11_1821 are separated by 49 nucleotides Ac3H11_1821 and Ac3H11_1822 are separated by 33 nucleotides Ac3H11_1822 and Ac3H11_1823 are separated by 12 nucleotides
Ac3H11_1818 - Dihydroneopterin aldolase (EC 4.1.2.25), at 23,274 to 23,648
_1818
Ac3H11_1819 - FolM Alternative dihydrofolate reductase 1, at 23,678 to 24,463
_1819
Ac3H11_1820 - COG1565: Uncharacterized conserved protein, at 24,479 to 25,597
_1820
Ac3H11_1821 - hypothetical protein, at 25,647 to 25,805
_1821
Ac3H11_1822 - Thioredoxin, at 25,839 to 26,306
_1822
Ac3H11_1823 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 26,319 to 26,792
_1823
Position (kb) 24
25
26 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 23.973 kb on + strand, within Ac3H11_1819 at 23.974 kb on - strand, within Ac3H11_1819 at 24.318 kb on - strand, within Ac3H11_1819 at 24.318 kb on - strand, within Ac3H11_1819 at 24.457 kb on + strand at 24.457 kb on + strand at 24.458 kb on - strand at 24.458 kb on - strand at 24.458 kb on - strand at 24.458 kb on - strand at 25.645 kb on + strand at 25.645 kb on + strand at 25.646 kb on - strand at 25.646 kb on - strand at 25.803 kb on + strand at 25.803 kb on + strand at 25.803 kb on + strand at 25.804 kb on - strand at 25.980 kb on - strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.304 kb on + strand at 26.305 kb on - strand at 26.377 kb on + strand, within Ac3H11_1823 at 26.377 kb on + strand, within Ac3H11_1823 at 26.377 kb on + strand, within Ac3H11_1823 at 26.378 kb on - strand, within Ac3H11_1823 at 26.378 kb on - strand, within Ac3H11_1823 at 26.378 kb on - strand, within Ac3H11_1823 at 26.425 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.576 kb on - strand, within Ac3H11_1823 at 26.596 kb on + strand, within Ac3H11_1823 at 26.596 kb on + strand, within Ac3H11_1823 at 26.596 kb on + strand, within Ac3H11_1823 at 26.597 kb on - strand, within Ac3H11_1823
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.001 mg/ml remove 23,973 + Ac3H11_1819 0.38 -0.7 23,974 - Ac3H11_1819 0.38 -0.2 24,318 - Ac3H11_1819 0.81 -0.7 24,318 - Ac3H11_1819 0.81 -0.5 24,457 + -2.7 24,457 + -1.8 24,458 - +0.0 24,458 - -0.0 24,458 - -0.0 24,458 - -0.2 25,645 + +1.0 25,645 + +0.6 25,646 - +0.6 25,646 - +0.2 25,803 + +0.4 25,803 + -0.2 25,803 + -0.4 25,804 - -0.2 25,980 - Ac3H11_1822 0.30 -2.1 26,187 + Ac3H11_1822 0.74 -0.3 26,187 + Ac3H11_1822 0.74 -0.6 26,187 + Ac3H11_1822 0.74 +0.4 26,187 + Ac3H11_1822 0.74 -1.1 26,187 + Ac3H11_1822 0.74 -0.3 26,304 + +1.2 26,305 - +0.4 26,377 + Ac3H11_1823 0.12 -0.1 26,377 + Ac3H11_1823 0.12 +0.4 26,377 + Ac3H11_1823 0.12 -0.7 26,378 - Ac3H11_1823 0.12 -0.7 26,378 - Ac3H11_1823 0.12 -0.0 26,378 - Ac3H11_1823 0.12 -0.3 26,425 + Ac3H11_1823 0.22 +0.4 26,575 + Ac3H11_1823 0.54 +0.4 26,575 + Ac3H11_1823 0.54 +0.0 26,575 + Ac3H11_1823 0.54 +0.5 26,575 + Ac3H11_1823 0.54 -0.7 26,575 + Ac3H11_1823 0.54 -0.4 26,576 - Ac3H11_1823 0.54 -3.2 26,596 + Ac3H11_1823 0.58 +0.8 26,596 + Ac3H11_1823 0.58 -2.3 26,596 + Ac3H11_1823 0.58 +0.8 26,597 - Ac3H11_1823 0.59 -1.2
Or see this region's nucleotide sequence