Experiment: Fusidic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1209 and Ac3H11_1210 are separated by 118 nucleotides Ac3H11_1210 and Ac3H11_1211 overlap by 4 nucleotides Ac3H11_1211 and Ac3H11_1212 are separated by 72 nucleotides
Ac3H11_1209 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 23,110 to 25,875
_1209
Ac3H11_1210 - transcriptional regulator, TetR family, at 25,994 to 26,725
_1210
Ac3H11_1211 - Outer membrane protein assembly factor YaeT precursor, at 26,722 to 28,170
_1211
Ac3H11_1212 - Secretion protein HlyD precursor, at 28,243 to 29,454
_1212
Position (kb) 26
27
28
29 Strain fitness (log2 ratio) -2
-1
0
1
2 at 25.873 kb on + strand at 25.874 kb on - strand at 25.982 kb on + strand at 25.983 kb on - strand at 26.201 kb on + strand, within Ac3H11_1210 at 26.201 kb on + strand, within Ac3H11_1210 at 26.201 kb on + strand, within Ac3H11_1210 at 26.201 kb on + strand, within Ac3H11_1210 at 26.216 kb on + strand, within Ac3H11_1210 at 26.216 kb on + strand, within Ac3H11_1210 at 26.217 kb on - strand, within Ac3H11_1210 at 26.297 kb on + strand, within Ac3H11_1210 at 26.298 kb on - strand, within Ac3H11_1210 at 26.298 kb on - strand, within Ac3H11_1210 at 26.488 kb on + strand, within Ac3H11_1210 at 26.489 kb on - strand, within Ac3H11_1210 at 27.587 kb on - strand, within Ac3H11_1211 at 27.587 kb on - strand, within Ac3H11_1211 at 27.832 kb on + strand, within Ac3H11_1211 at 28.088 kb on - strand at 28.328 kb on - strand at 28.328 kb on - strand at 28.792 kb on + strand, within Ac3H11_1212 at 28.792 kb on + strand, within Ac3H11_1212 at 28.793 kb on - strand, within Ac3H11_1212 at 29.056 kb on + strand, within Ac3H11_1212 at 29.056 kb on + strand, within Ac3H11_1212 at 29.056 kb on + strand, within Ac3H11_1212 at 29.056 kb on + strand, within Ac3H11_1212 at 29.056 kb on + strand, within Ac3H11_1212 at 29.056 kb on + strand, within Ac3H11_1212 at 29.056 kb on + strand, within Ac3H11_1212 at 29.057 kb on - strand, within Ac3H11_1212 at 29.057 kb on - strand, within Ac3H11_1212 at 29.057 kb on - strand, within Ac3H11_1212 at 29.057 kb on - strand, within Ac3H11_1212
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.001 mg/ml remove 25,873 + -0.7 25,874 - -0.7 25,982 + -0.1 25,983 - -0.6 26,201 + Ac3H11_1210 0.28 -0.9 26,201 + Ac3H11_1210 0.28 -1.7 26,201 + Ac3H11_1210 0.28 -1.6 26,201 + Ac3H11_1210 0.28 -0.2 26,216 + Ac3H11_1210 0.30 +0.5 26,216 + Ac3H11_1210 0.30 +0.2 26,217 - Ac3H11_1210 0.30 -0.4 26,297 + Ac3H11_1210 0.41 -1.0 26,298 - Ac3H11_1210 0.42 -2.6 26,298 - Ac3H11_1210 0.42 -1.4 26,488 + Ac3H11_1210 0.67 -0.8 26,489 - Ac3H11_1210 0.68 -1.0 27,587 - Ac3H11_1211 0.60 +0.3 27,587 - Ac3H11_1211 0.60 +0.2 27,832 + Ac3H11_1211 0.77 -0.5 28,088 - +0.0 28,328 - +2.1 28,328 - -0.6 28,792 + Ac3H11_1212 0.45 +0.1 28,792 + Ac3H11_1212 0.45 +0.5 28,793 - Ac3H11_1212 0.45 +0.5 29,056 + Ac3H11_1212 0.67 -1.0 29,056 + Ac3H11_1212 0.67 -0.4 29,056 + Ac3H11_1212 0.67 +0.3 29,056 + Ac3H11_1212 0.67 -0.7 29,056 + Ac3H11_1212 0.67 -1.6 29,056 + Ac3H11_1212 0.67 +0.4 29,056 + Ac3H11_1212 0.67 +0.8 29,057 - Ac3H11_1212 0.67 -0.1 29,057 - Ac3H11_1212 0.67 +0.1 29,057 - Ac3H11_1212 0.67 -0.8 29,057 - Ac3H11_1212 0.67 +0.6
Or see this region's nucleotide sequence