Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0055 and Shew_R0056 are separated by 39 nucleotides Shew_R0056 and Shew_1530 are separated by 656 nucleotides Shew_1530 and grxA are separated by 53 nucleotides grxA and Shew_1532 are separated by 65 nucleotides
Shew_R0055: Shew_R0055 - tRNA-Lys (RefSeq), at 1,753,864 to 1,753,939
_R0055
Shew_R0056: Shew_R0056 - tRNA-Lys (RefSeq), at 1,753,979 to 1,754,054
_R0056
Shew_1530: Shew_1530 - porin (RefSeq), at 1,754,711 to 1,755,853
_1530
Shew_1531: grxA - glutaredoxin 1 (RefSeq), at 1,755,907 to 1,756,164
grxA
Shew_1532: Shew_1532 - type III restriction enzyme, res subunit (RefSeq), at 1,756,230 to 1,757,969
_1532
Position (kb)
1754
1755
1756 Strain fitness (log2 ratio)
-2
-1
0
1 at 1754.182 kb on + strand at 1754.190 kb on - strand at 1754.335 kb on + strand at 1754.617 kb on + strand at 1754.799 kb on - strand at 1754.815 kb on + strand at 1754.996 kb on - strand, within Shew_1530 at 1755.354 kb on - strand, within Shew_1530 at 1755.730 kb on + strand, within Shew_1530 at 1755.756 kb on - strand at 1755.767 kb on + strand at 1756.001 kb on + strand, within grxA at 1756.124 kb on - strand, within grxA at 1756.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 1,754,182 + +0.4 1,754,190 - +0.1 1,754,335 + -1.2 1,754,617 + -1.9 1,754,799 - -0.7 1,754,815 + -0.3 1,754,996 - Shew_1530 0.25 +1.3 1,755,354 - Shew_1530 0.56 +0.2 1,755,730 + Shew_1530 0.89 -0.9 1,755,756 - +0.0 1,755,767 + +1.4 1,756,001 + grxA Shew_1531 0.36 +1.2 1,756,124 - grxA Shew_1531 0.84 +1.5 1,756,206 - +1.3
Or see this region's nucleotide sequence