Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0306 and Shew_R0014 are separated by 218 nucleotides Shew_R0014 and Shew_R0015 are separated by 43 nucleotides Shew_R0015 and Shew_R0016 are separated by 84 nucleotides Shew_R0016 and Shew_R0017 are separated by 52 nucleotides Shew_R0017 and Shew_0307 are separated by 132 nucleotides
Shew_0306: Shew_0306 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 329,116 to 331,764
_0306
Shew_R0014: Shew_R0014 - tRNA-Arg (RefSeq), at 331,983 to 332,059
_R0014
Shew_R0015: Shew_R0015 - tRNA-His (RefSeq), at 332,103 to 332,178
_R0015
Shew_R0016: Shew_R0016 - tRNA-Pro (RefSeq), at 332,263 to 332,339
_R0016
Shew_R0017: Shew_R0017 - tRNA-Pro (RefSeq), at 332,392 to 332,468
_R0017
Shew_0307: Shew_0307 - diguanylate cyclase/phosphodiesterase (RefSeq), at 332,601 to 334,523
_0307
Position (kb) 331
332
333 Strain fitness (log2 ratio) -2
-1
0
1 at 331.026 kb on - strand, within Shew_0306 at 331.080 kb on + strand, within Shew_0306 at 331.084 kb on - strand, within Shew_0306 at 331.094 kb on - strand, within Shew_0306 at 331.099 kb on - strand, within Shew_0306 at 331.164 kb on - strand, within Shew_0306 at 331.176 kb on + strand, within Shew_0306 at 331.407 kb on - strand, within Shew_0306 at 331.484 kb on - strand, within Shew_0306 at 331.986 kb on + strand at 332.850 kb on - strand, within Shew_0307
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 331,026 - Shew_0306 0.72 +0.4 331,080 + Shew_0306 0.74 -0.9 331,084 - Shew_0306 0.74 +0.3 331,094 - Shew_0306 0.75 +0.3 331,099 - Shew_0306 0.75 -1.4 331,164 - Shew_0306 0.77 -0.6 331,176 + Shew_0306 0.78 -0.5 331,407 - Shew_0306 0.86 +0.2 331,484 - Shew_0306 0.89 -0.0 331,986 + -2.5 332,850 - Shew_0307 0.13 -0.9
Or see this region's nucleotide sequence