Experiment: Chlorite 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0026 and Shew_0027 are separated by 12 nucleotides Shew_0027 and Shew_0028 are separated by 81 nucleotides Shew_0028 and Shew_0029 are separated by 229 nucleotides
Shew_0026: Shew_0026 - hypothetical protein (RefSeq), at 28,832 to 29,368
_0026
Shew_0027: Shew_0027 - glutathione S-transferase domain-containing protein (RefSeq), at 29,381 to 30,028
_0027
Shew_0028: Shew_0028 - LysR family transcriptional regulator (RefSeq), at 30,110 to 31,009
_0028
Shew_0029: Shew_0029 - 3-methyladenine DNA glycosylase (RefSeq), at 31,239 to 31,928
_0029
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-1
0
1 at 29.135 kb on + strand, within Shew_0026 at 29.328 kb on - strand at 29.371 kb on - strand at 29.774 kb on - strand, within Shew_0027 at 29.889 kb on + strand, within Shew_0027 at 30.115 kb on - strand at 30.128 kb on - strand at 30.324 kb on - strand, within Shew_0028 at 30.531 kb on + strand, within Shew_0028 at 30.539 kb on - strand, within Shew_0028 at 30.559 kb on - strand, within Shew_0028 at 30.572 kb on - strand, within Shew_0028 at 30.688 kb on - strand, within Shew_0028 at 30.780 kb on + strand, within Shew_0028 at 30.790 kb on + strand, within Shew_0028 at 30.951 kb on - strand at 31.272 kb on - strand at 31.658 kb on + strand, within Shew_0029 at 31.658 kb on + strand, within Shew_0029 at 31.658 kb on + strand, within Shew_0029 at 31.658 kb on + strand, within Shew_0029 at 31.666 kb on - strand, within Shew_0029 at 31.893 kb on + strand at 31.908 kb on - strand at 31.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chlorite 200 mM remove 29,135 + Shew_0026 0.56 +0.3 29,328 - +0.1 29,371 - +1.5 29,774 - Shew_0027 0.61 -0.1 29,889 + Shew_0027 0.78 +0.1 30,115 - +0.1 30,128 - -0.7 30,324 - Shew_0028 0.24 +0.5 30,531 + Shew_0028 0.47 +0.1 30,539 - Shew_0028 0.48 +0.5 30,559 - Shew_0028 0.50 -0.6 30,572 - Shew_0028 0.51 -0.0 30,688 - Shew_0028 0.64 +0.5 30,780 + Shew_0028 0.74 +0.2 30,790 + Shew_0028 0.76 +0.5 30,951 - -0.4 31,272 - +0.1 31,658 + Shew_0029 0.61 -1.2 31,658 + Shew_0029 0.61 -0.3 31,658 + Shew_0029 0.61 -0.0 31,658 + Shew_0029 0.61 -0.2 31,666 - Shew_0029 0.62 -0.3 31,893 + -0.3 31,908 - -0.1 31,936 - -0.1
Or see this region's nucleotide sequence