Experiment: Cephalothin 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5372 and Pf6N2E2_5373 overlap by 11 nucleotides Pf6N2E2_5373 and Pf6N2E2_5374 are separated by 10 nucleotides
Pf6N2E2_5372 - Inner membrane component of tripartite multidrug resistance system, at 6,173,011 to 6,175,143
_5372
Pf6N2E2_5373 - required for cephalothin efflux, together with NodT, MFP and FUSC proteins (Pf6N2E2_5371, Pf6N2E2_5374, Pf6N2E2_5372) (from data) , at 6,175,133 to 6,175,333
_5373
Pf6N2E2_5374 - Membrane fusion component of tripartite multidrug resistance system, at 6,175,344 to 6,176,207
_5374
Position (kb)
6175
6176 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 6174.482 kb on - strand, within Pf6N2E2_5372 at 6174.499 kb on - strand, within Pf6N2E2_5372 at 6174.508 kb on + strand, within Pf6N2E2_5372 at 6174.508 kb on + strand, within Pf6N2E2_5372 at 6174.509 kb on - strand, within Pf6N2E2_5372 at 6174.509 kb on - strand, within Pf6N2E2_5372 at 6174.509 kb on - strand, within Pf6N2E2_5372 at 6174.812 kb on + strand, within Pf6N2E2_5372 at 6174.830 kb on - strand, within Pf6N2E2_5372 at 6174.830 kb on - strand, within Pf6N2E2_5372 at 6174.830 kb on - strand, within Pf6N2E2_5372 at 6175.024 kb on + strand at 6175.024 kb on + strand at 6175.025 kb on - strand at 6175.078 kb on + strand at 6175.085 kb on + strand at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.163 kb on + strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.164 kb on - strand, within Pf6N2E2_5373 at 6175.291 kb on + strand, within Pf6N2E2_5373 at 6175.291 kb on + strand, within Pf6N2E2_5373 at 6175.292 kb on - strand, within Pf6N2E2_5373 at 6175.325 kb on + strand at 6175.325 kb on + strand at 6175.414 kb on + strand at 6175.425 kb on + strand at 6175.425 kb on + strand at 6175.425 kb on + strand at 6175.425 kb on + strand at 6175.425 kb on + strand at 6175.425 kb on + strand at 6175.425 kb on + strand at 6175.425 kb on + strand at 6175.425 kb on + strand at 6175.425 kb on + strand at 6175.426 kb on - strand at 6175.426 kb on - strand at 6175.426 kb on - strand at 6175.426 kb on - strand at 6175.426 kb on - strand at 6175.426 kb on - strand at 6175.426 kb on - strand at 6175.426 kb on - strand at 6175.426 kb on - strand at 6175.821 kb on + strand, within Pf6N2E2_5374 at 6175.911 kb on + strand, within Pf6N2E2_5374 at 6175.911 kb on + strand, within Pf6N2E2_5374 at 6175.912 kb on - strand, within Pf6N2E2_5374 at 6175.912 kb on - strand, within Pf6N2E2_5374 at 6175.912 kb on - strand, within Pf6N2E2_5374 at 6176.028 kb on + strand, within Pf6N2E2_5374 at 6176.028 kb on + strand, within Pf6N2E2_5374 at 6176.029 kb on - strand, within Pf6N2E2_5374 at 6176.029 kb on - strand, within Pf6N2E2_5374 at 6176.137 kb on + strand at 6176.310 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 4 mg/ml remove 6,174,482 - Pf6N2E2_5372 0.69 -1.3 6,174,499 - Pf6N2E2_5372 0.70 -1.1 6,174,508 + Pf6N2E2_5372 0.70 -4.1 6,174,508 + Pf6N2E2_5372 0.70 -3.1 6,174,509 - Pf6N2E2_5372 0.70 -2.3 6,174,509 - Pf6N2E2_5372 0.70 -4.7 6,174,509 - Pf6N2E2_5372 0.70 -2.3 6,174,812 + Pf6N2E2_5372 0.84 -3.3 6,174,830 - Pf6N2E2_5372 0.85 -1.7 6,174,830 - Pf6N2E2_5372 0.85 -2.5 6,174,830 - Pf6N2E2_5372 0.85 -2.3 6,175,024 + -2.4 6,175,024 + -0.9 6,175,025 - -2.4 6,175,078 + +0.8 6,175,085 + -0.5 6,175,163 + Pf6N2E2_5373 0.15 -3.5 6,175,163 + Pf6N2E2_5373 0.15 -3.4 6,175,163 + Pf6N2E2_5373 0.15 -3.7 6,175,163 + Pf6N2E2_5373 0.15 -0.9 6,175,163 + Pf6N2E2_5373 0.15 +0.1 6,175,163 + Pf6N2E2_5373 0.15 +1.1 6,175,163 + Pf6N2E2_5373 0.15 -1.7 6,175,163 + Pf6N2E2_5373 0.15 -1.4 6,175,163 + Pf6N2E2_5373 0.15 -2.6 6,175,163 + Pf6N2E2_5373 0.15 +0.1 6,175,163 + Pf6N2E2_5373 0.15 -1.4 6,175,163 + Pf6N2E2_5373 0.15 -2.7 6,175,163 + Pf6N2E2_5373 0.15 -1.3 6,175,163 + Pf6N2E2_5373 0.15 -1.9 6,175,164 - Pf6N2E2_5373 0.15 -1.4 6,175,164 - Pf6N2E2_5373 0.15 -4.5 6,175,164 - Pf6N2E2_5373 0.15 -3.6 6,175,164 - Pf6N2E2_5373 0.15 -3.3 6,175,164 - Pf6N2E2_5373 0.15 -2.3 6,175,164 - Pf6N2E2_5373 0.15 -0.4 6,175,164 - Pf6N2E2_5373 0.15 -1.5 6,175,164 - Pf6N2E2_5373 0.15 -2.4 6,175,164 - Pf6N2E2_5373 0.15 -3.6 6,175,164 - Pf6N2E2_5373 0.15 -2.5 6,175,164 - Pf6N2E2_5373 0.15 -2.0 6,175,164 - Pf6N2E2_5373 0.15 -0.9 6,175,164 - Pf6N2E2_5373 0.15 -2.8 6,175,164 - Pf6N2E2_5373 0.15 -2.2 6,175,164 - Pf6N2E2_5373 0.15 -2.3 6,175,164 - Pf6N2E2_5373 0.15 -3.9 6,175,164 - Pf6N2E2_5373 0.15 -3.0 6,175,291 + Pf6N2E2_5373 0.79 -3.6 6,175,291 + Pf6N2E2_5373 0.79 -2.3 6,175,292 - Pf6N2E2_5373 0.79 -2.7 6,175,325 + -0.3 6,175,325 + +0.1 6,175,414 + -5.1 6,175,425 + -4.0 6,175,425 + -0.9 6,175,425 + -2.0 6,175,425 + -3.7 6,175,425 + -2.9 6,175,425 + -3.0 6,175,425 + -3.9 6,175,425 + -2.1 6,175,425 + -1.7 6,175,425 + -3.4 6,175,426 - -1.3 6,175,426 - -2.6 6,175,426 - -2.7 6,175,426 - -1.3 6,175,426 - -3.5 6,175,426 - -4.6 6,175,426 - -3.2 6,175,426 - -3.8 6,175,426 - -2.5 6,175,821 + Pf6N2E2_5374 0.55 -1.5 6,175,911 + Pf6N2E2_5374 0.66 -2.5 6,175,911 + Pf6N2E2_5374 0.66 -3.3 6,175,912 - Pf6N2E2_5374 0.66 -3.8 6,175,912 - Pf6N2E2_5374 0.66 -1.5 6,175,912 - Pf6N2E2_5374 0.66 -2.8 6,176,028 + Pf6N2E2_5374 0.79 -2.4 6,176,028 + Pf6N2E2_5374 0.79 -3.5 6,176,029 - Pf6N2E2_5374 0.79 -3.0 6,176,029 - Pf6N2E2_5374 0.79 -2.8 6,176,137 + -3.0 6,176,310 + -0.5
Or see this region's nucleotide sequence