Experiment: Cephalothin 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1975 and Pf6N2E2_1976 are separated by 11 nucleotides Pf6N2E2_1976 and Pf6N2E2_1977 are separated by 216 nucleotides Pf6N2E2_1977 and Pf6N2E2_1978 are separated by 8 nucleotides Pf6N2E2_1978 and Pf6N2E2_1979 are separated by 461 nucleotides
Pf6N2E2_1975 - Integrase regulator R, at 2,341,340 to 2,341,840
_1975
Pf6N2E2_1976 - Single-stranded DNA-binding protein, at 2,341,852 to 2,342,256
_1976
Pf6N2E2_1977 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,342,473 to 2,344,023
_1977
Pf6N2E2_1978 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,344,032 to 2,344,559
_1978
Pf6N2E2_1979 - Cytosine-specific DNA methyltransferase (EC 2.1.1.37), at 2,345,021 to 2,346,277
_1979
Position (kb)
2342
2343
2344
2345 Strain fitness (log2 ratio)
-2
-1
0
1 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.805 kb on + strand at 2341.806 kb on - strand at 2341.836 kb on + strand at 2341.836 kb on + strand at 2341.837 kb on - strand at 2341.837 kb on - strand at 2341.879 kb on - strand at 2341.879 kb on - strand at 2341.882 kb on - strand at 2342.595 kb on - strand at 2342.695 kb on + strand, within Pf6N2E2_1977 at 2342.696 kb on - strand, within Pf6N2E2_1977 at 2342.696 kb on - strand, within Pf6N2E2_1977 at 2342.808 kb on - strand, within Pf6N2E2_1977 at 2342.999 kb on - strand, within Pf6N2E2_1977 at 2343.090 kb on + strand, within Pf6N2E2_1977 at 2343.131 kb on + strand, within Pf6N2E2_1977 at 2343.131 kb on + strand, within Pf6N2E2_1977 at 2343.132 kb on - strand, within Pf6N2E2_1977 at 2343.138 kb on - strand, within Pf6N2E2_1977 at 2343.212 kb on + strand, within Pf6N2E2_1977 at 2343.212 kb on + strand, within Pf6N2E2_1977 at 2343.342 kb on - strand, within Pf6N2E2_1977 at 2343.472 kb on + strand, within Pf6N2E2_1977 at 2343.727 kb on + strand, within Pf6N2E2_1977 at 2343.727 kb on + strand, within Pf6N2E2_1977 at 2343.748 kb on + strand, within Pf6N2E2_1977 at 2343.779 kb on - strand, within Pf6N2E2_1977 at 2343.818 kb on + strand, within Pf6N2E2_1977 at 2343.819 kb on - strand, within Pf6N2E2_1977 at 2343.819 kb on - strand, within Pf6N2E2_1977 at 2343.972 kb on + strand at 2343.973 kb on - strand at 2344.005 kb on + strand at 2344.124 kb on + strand, within Pf6N2E2_1978 at 2344.152 kb on + strand, within Pf6N2E2_1978 at 2344.153 kb on - strand, within Pf6N2E2_1978 at 2344.153 kb on - strand, within Pf6N2E2_1978 at 2344.158 kb on + strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.659 kb on - strand at 2344.659 kb on - strand at 2344.749 kb on + strand at 2344.749 kb on + strand at 2344.749 kb on + strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2344.750 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 4 mg/ml remove 2,341,727 - Pf6N2E2_1975 0.77 -1.5 2,341,727 - Pf6N2E2_1975 0.77 +0.4 2,341,805 + -0.2 2,341,806 - +1.7 2,341,836 + +0.1 2,341,836 + -0.2 2,341,837 - +1.0 2,341,837 - +0.5 2,341,879 - +1.1 2,341,879 - -0.4 2,341,882 - -0.7 2,342,595 - -1.8 2,342,695 + Pf6N2E2_1977 0.14 -0.5 2,342,696 - Pf6N2E2_1977 0.14 -0.5 2,342,696 - Pf6N2E2_1977 0.14 -0.1 2,342,808 - Pf6N2E2_1977 0.22 +1.0 2,342,999 - Pf6N2E2_1977 0.34 +0.5 2,343,090 + Pf6N2E2_1977 0.40 -0.1 2,343,131 + Pf6N2E2_1977 0.42 -0.2 2,343,131 + Pf6N2E2_1977 0.42 -0.4 2,343,132 - Pf6N2E2_1977 0.42 +1.0 2,343,138 - Pf6N2E2_1977 0.43 +0.1 2,343,212 + Pf6N2E2_1977 0.48 -0.3 2,343,212 + Pf6N2E2_1977 0.48 -0.5 2,343,342 - Pf6N2E2_1977 0.56 +0.9 2,343,472 + Pf6N2E2_1977 0.64 -0.5 2,343,727 + Pf6N2E2_1977 0.81 +0.1 2,343,727 + Pf6N2E2_1977 0.81 +0.6 2,343,748 + Pf6N2E2_1977 0.82 +0.4 2,343,779 - Pf6N2E2_1977 0.84 -0.0 2,343,818 + Pf6N2E2_1977 0.87 -0.5 2,343,819 - Pf6N2E2_1977 0.87 +0.4 2,343,819 - Pf6N2E2_1977 0.87 -1.4 2,343,972 + +0.3 2,343,973 - -0.1 2,344,005 + +1.3 2,344,124 + Pf6N2E2_1978 0.17 +0.8 2,344,152 + Pf6N2E2_1978 0.23 +0.1 2,344,153 - Pf6N2E2_1978 0.23 -1.1 2,344,153 - Pf6N2E2_1978 0.23 -0.6 2,344,158 + Pf6N2E2_1978 0.24 -0.1 2,344,159 - Pf6N2E2_1978 0.24 +0.3 2,344,159 - Pf6N2E2_1978 0.24 -0.3 2,344,159 - Pf6N2E2_1978 0.24 +1.6 2,344,159 - Pf6N2E2_1978 0.24 +0.7 2,344,159 - Pf6N2E2_1978 0.24 -1.5 2,344,659 - -0.9 2,344,659 - +1.3 2,344,749 + -0.0 2,344,749 + -0.2 2,344,749 + +0.4 2,344,750 - -0.5 2,344,750 - -0.5 2,344,750 - -0.3 2,344,750 - +1.1
Or see this region's nucleotide sequence