Experiment: Cephalothin 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1196 and Pf6N2E2_1197 overlap by 1 nucleotides Pf6N2E2_1197 and Pf6N2E2_1198 are separated by 22 nucleotides
Pf6N2E2_1196 - hypothetical protein, at 1,397,832 to 1,399,154
_1196
Pf6N2E2_1197 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl diphosphate synthase (EC 2.5.1.29), at 1,399,154 to 1,400,218
_1197
Pf6N2E2_1198 - hypothetical protein, at 1,400,241 to 1,401,875
_1198
Position (kb)
1399
1400
1401 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1398.222 kb on - strand, within Pf6N2E2_1196 at 1398.312 kb on - strand, within Pf6N2E2_1196 at 1398.312 kb on - strand, within Pf6N2E2_1196 at 1398.416 kb on + strand, within Pf6N2E2_1196 at 1398.417 kb on - strand, within Pf6N2E2_1196 at 1398.499 kb on - strand, within Pf6N2E2_1196 at 1398.534 kb on + strand, within Pf6N2E2_1196 at 1398.547 kb on - strand, within Pf6N2E2_1196 at 1398.639 kb on - strand, within Pf6N2E2_1196 at 1398.657 kb on + strand, within Pf6N2E2_1196 at 1398.658 kb on - strand, within Pf6N2E2_1196 at 1398.779 kb on - strand, within Pf6N2E2_1196 at 1398.837 kb on + strand, within Pf6N2E2_1196 at 1398.838 kb on - strand, within Pf6N2E2_1196 at 1399.003 kb on + strand, within Pf6N2E2_1196 at 1399.179 kb on + strand at 1399.180 kb on - strand at 1399.221 kb on - strand at 1399.221 kb on - strand at 1399.421 kb on + strand, within Pf6N2E2_1197 at 1399.421 kb on + strand, within Pf6N2E2_1197 at 1399.422 kb on - strand, within Pf6N2E2_1197 at 1399.422 kb on - strand, within Pf6N2E2_1197 at 1399.422 kb on - strand, within Pf6N2E2_1197 at 1399.572 kb on + strand, within Pf6N2E2_1197 at 1399.657 kb on + strand, within Pf6N2E2_1197 at 1399.727 kb on + strand, within Pf6N2E2_1197 at 1399.727 kb on + strand, within Pf6N2E2_1197 at 1399.731 kb on - strand, within Pf6N2E2_1197 at 1399.731 kb on - strand, within Pf6N2E2_1197 at 1399.935 kb on + strand, within Pf6N2E2_1197 at 1399.964 kb on + strand, within Pf6N2E2_1197 at 1399.964 kb on + strand, within Pf6N2E2_1197 at 1400.133 kb on - strand at 1400.187 kb on + strand at 1400.188 kb on - strand at 1400.311 kb on + strand at 1400.312 kb on - strand at 1400.398 kb on - strand at 1400.421 kb on + strand, within Pf6N2E2_1198 at 1400.421 kb on + strand, within Pf6N2E2_1198 at 1400.422 kb on - strand, within Pf6N2E2_1198 at 1400.422 kb on - strand, within Pf6N2E2_1198 at 1400.422 kb on - strand, within Pf6N2E2_1198 at 1400.446 kb on + strand, within Pf6N2E2_1198 at 1400.447 kb on - strand, within Pf6N2E2_1198 at 1400.447 kb on - strand, within Pf6N2E2_1198 at 1400.447 kb on - strand, within Pf6N2E2_1198 at 1400.497 kb on - strand, within Pf6N2E2_1198 at 1400.616 kb on + strand, within Pf6N2E2_1198 at 1400.617 kb on - strand, within Pf6N2E2_1198 at 1400.617 kb on - strand, within Pf6N2E2_1198 at 1400.619 kb on + strand, within Pf6N2E2_1198 at 1400.619 kb on + strand, within Pf6N2E2_1198 at 1400.620 kb on - strand, within Pf6N2E2_1198 at 1400.620 kb on - strand, within Pf6N2E2_1198 at 1400.620 kb on - strand, within Pf6N2E2_1198 at 1400.622 kb on - strand, within Pf6N2E2_1198 at 1401.045 kb on - strand, within Pf6N2E2_1198 at 1401.201 kb on - strand, within Pf6N2E2_1198 at 1401.201 kb on - strand, within Pf6N2E2_1198 at 1401.207 kb on + strand, within Pf6N2E2_1198 at 1401.207 kb on + strand, within Pf6N2E2_1198
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 4 mg/ml remove 1,398,222 - Pf6N2E2_1196 0.29 +0.8 1,398,312 - Pf6N2E2_1196 0.36 +0.1 1,398,312 - Pf6N2E2_1196 0.36 -1.0 1,398,416 + Pf6N2E2_1196 0.44 +0.2 1,398,417 - Pf6N2E2_1196 0.44 +0.0 1,398,499 - Pf6N2E2_1196 0.50 +0.6 1,398,534 + Pf6N2E2_1196 0.53 -0.6 1,398,547 - Pf6N2E2_1196 0.54 -1.3 1,398,639 - Pf6N2E2_1196 0.61 +0.2 1,398,657 + Pf6N2E2_1196 0.62 +0.4 1,398,658 - Pf6N2E2_1196 0.62 -0.9 1,398,779 - Pf6N2E2_1196 0.72 -1.2 1,398,837 + Pf6N2E2_1196 0.76 +0.8 1,398,838 - Pf6N2E2_1196 0.76 +0.8 1,399,003 + Pf6N2E2_1196 0.89 -0.1 1,399,179 + +0.8 1,399,180 - +0.1 1,399,221 - -0.5 1,399,221 - -0.3 1,399,421 + Pf6N2E2_1197 0.25 +1.2 1,399,421 + Pf6N2E2_1197 0.25 +0.6 1,399,422 - Pf6N2E2_1197 0.25 +2.6 1,399,422 - Pf6N2E2_1197 0.25 +0.7 1,399,422 - Pf6N2E2_1197 0.25 +0.6 1,399,572 + Pf6N2E2_1197 0.39 +2.6 1,399,657 + Pf6N2E2_1197 0.47 -0.3 1,399,727 + Pf6N2E2_1197 0.54 +0.7 1,399,727 + Pf6N2E2_1197 0.54 +1.4 1,399,731 - Pf6N2E2_1197 0.54 -0.8 1,399,731 - Pf6N2E2_1197 0.54 +1.4 1,399,935 + Pf6N2E2_1197 0.73 +0.7 1,399,964 + Pf6N2E2_1197 0.76 -0.8 1,399,964 + Pf6N2E2_1197 0.76 +0.4 1,400,133 - -0.9 1,400,187 + +0.8 1,400,188 - +0.5 1,400,311 + +0.5 1,400,312 - -0.5 1,400,398 - -1.2 1,400,421 + Pf6N2E2_1198 0.11 -0.0 1,400,421 + Pf6N2E2_1198 0.11 -0.8 1,400,422 - Pf6N2E2_1198 0.11 +0.8 1,400,422 - Pf6N2E2_1198 0.11 -0.2 1,400,422 - Pf6N2E2_1198 0.11 -2.8 1,400,446 + Pf6N2E2_1198 0.13 -0.5 1,400,447 - Pf6N2E2_1198 0.13 +0.4 1,400,447 - Pf6N2E2_1198 0.13 -1.0 1,400,447 - Pf6N2E2_1198 0.13 +1.2 1,400,497 - Pf6N2E2_1198 0.16 -0.2 1,400,616 + Pf6N2E2_1198 0.23 -0.0 1,400,617 - Pf6N2E2_1198 0.23 +0.5 1,400,617 - Pf6N2E2_1198 0.23 +0.4 1,400,619 + Pf6N2E2_1198 0.23 +1.1 1,400,619 + Pf6N2E2_1198 0.23 -1.5 1,400,620 - Pf6N2E2_1198 0.23 -0.6 1,400,620 - Pf6N2E2_1198 0.23 +1.4 1,400,620 - Pf6N2E2_1198 0.23 +0.4 1,400,622 - Pf6N2E2_1198 0.23 -0.0 1,401,045 - Pf6N2E2_1198 0.49 -1.5 1,401,201 - Pf6N2E2_1198 0.59 +0.3 1,401,201 - Pf6N2E2_1198 0.59 +0.5 1,401,207 + Pf6N2E2_1198 0.59 +1.5 1,401,207 + Pf6N2E2_1198 0.59 +0.2
Or see this region's nucleotide sequence