Experiment: Cephalothin 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1125 and Pf6N2E2_1126 are separated by 32 nucleotides Pf6N2E2_1126 and Pf6N2E2_1127 overlap by 4 nucleotides Pf6N2E2_1127 and Pf6N2E2_1128 overlap by 10 nucleotides Pf6N2E2_1128 and Pf6N2E2_1129 overlap by 4 nucleotides
Pf6N2E2_1125 - Transcriptional regulator, AraC family, at 1,320,921 to 1,321,817
_1125
Pf6N2E2_1126 - Two-component system regulatory protein, at 1,321,850 to 1,322,317
_1126
Pf6N2E2_1127 - Sensory box histidine kinase/response regulator, at 1,322,314 to 1,324,632
_1127
Pf6N2E2_1128 - Two-component response regulator, at 1,324,623 to 1,325,291
_1128
Pf6N2E2_1129 - Sensor histidine kinase, at 1,325,288 to 1,326,472
_1129
Position (kb)
1322
1323
1324
1325 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1321.484 kb on - strand, within Pf6N2E2_1125 at 1321.537 kb on + strand, within Pf6N2E2_1125 at 1321.538 kb on - strand, within Pf6N2E2_1125 at 1321.927 kb on + strand, within Pf6N2E2_1126 at 1321.927 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.930 kb on - strand, within Pf6N2E2_1126 at 1321.930 kb on - strand, within Pf6N2E2_1126 at 1321.930 kb on - strand, within Pf6N2E2_1126 at 1322.080 kb on - strand, within Pf6N2E2_1126 at 1322.330 kb on - strand at 1322.333 kb on + strand at 1322.333 kb on + strand at 1322.334 kb on - strand at 1322.365 kb on - strand at 1322.370 kb on + strand at 1322.370 kb on + strand at 1322.370 kb on + strand at 1322.370 kb on + strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.749 kb on + strand, within Pf6N2E2_1127 at 1322.749 kb on + strand, within Pf6N2E2_1127 at 1322.749 kb on + strand, within Pf6N2E2_1127 at 1323.026 kb on + strand, within Pf6N2E2_1127 at 1323.026 kb on + strand, within Pf6N2E2_1127 at 1323.294 kb on + strand, within Pf6N2E2_1127 at 1323.295 kb on - strand, within Pf6N2E2_1127 at 1323.533 kb on + strand, within Pf6N2E2_1127 at 1323.533 kb on + strand, within Pf6N2E2_1127 at 1323.605 kb on + strand, within Pf6N2E2_1127 at 1323.605 kb on + strand, within Pf6N2E2_1127 at 1323.605 kb on + strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.606 kb on - strand, within Pf6N2E2_1127 at 1323.671 kb on + strand, within Pf6N2E2_1127 at 1323.672 kb on - strand, within Pf6N2E2_1127 at 1323.672 kb on - strand, within Pf6N2E2_1127 at 1323.714 kb on - strand, within Pf6N2E2_1127 at 1323.756 kb on - strand, within Pf6N2E2_1127 at 1323.899 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.362 kb on - strand, within Pf6N2E2_1127 at 1324.397 kb on + strand, within Pf6N2E2_1127 at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.405 kb on + strand at 1324.406 kb on - strand at 1324.435 kb on - strand at 1324.516 kb on - strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.517 kb on + strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.518 kb on - strand at 1324.726 kb on - strand, within Pf6N2E2_1128 at 1324.982 kb on - strand, within Pf6N2E2_1128 at 1325.231 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 4 mg/ml remove 1,321,484 - Pf6N2E2_1125 0.63 +1.1 1,321,537 + Pf6N2E2_1125 0.69 +0.8 1,321,538 - Pf6N2E2_1125 0.69 +0.0 1,321,927 + Pf6N2E2_1126 0.16 -0.8 1,321,927 + Pf6N2E2_1126 0.16 +0.3 1,321,929 + Pf6N2E2_1126 0.17 -0.5 1,321,929 + Pf6N2E2_1126 0.17 +2.3 1,321,929 + Pf6N2E2_1126 0.17 +0.4 1,321,929 + Pf6N2E2_1126 0.17 +1.2 1,321,930 - Pf6N2E2_1126 0.17 -1.4 1,321,930 - Pf6N2E2_1126 0.17 +0.3 1,321,930 - Pf6N2E2_1126 0.17 +0.3 1,322,080 - Pf6N2E2_1126 0.49 +1.0 1,322,330 - +0.0 1,322,333 + +0.0 1,322,333 + +0.0 1,322,334 - +0.8 1,322,365 - -0.8 1,322,370 + -0.0 1,322,370 + -0.0 1,322,370 + +0.6 1,322,370 + -1.8 1,322,371 - +1.2 1,322,371 - -0.2 1,322,371 - -0.2 1,322,371 - +0.2 1,322,371 - -0.5 1,322,749 + Pf6N2E2_1127 0.19 -0.3 1,322,749 + Pf6N2E2_1127 0.19 -0.5 1,322,749 + Pf6N2E2_1127 0.19 +1.0 1,323,026 + Pf6N2E2_1127 0.31 +0.2 1,323,026 + Pf6N2E2_1127 0.31 -0.8 1,323,294 + Pf6N2E2_1127 0.42 +0.1 1,323,295 - Pf6N2E2_1127 0.42 +0.6 1,323,533 + Pf6N2E2_1127 0.53 +1.0 1,323,533 + Pf6N2E2_1127 0.53 +0.3 1,323,605 + Pf6N2E2_1127 0.56 +0.3 1,323,605 + Pf6N2E2_1127 0.56 -0.3 1,323,605 + Pf6N2E2_1127 0.56 +0.4 1,323,606 - Pf6N2E2_1127 0.56 +0.3 1,323,606 - Pf6N2E2_1127 0.56 +0.8 1,323,606 - Pf6N2E2_1127 0.56 -1.8 1,323,606 - Pf6N2E2_1127 0.56 -0.2 1,323,606 - Pf6N2E2_1127 0.56 +0.5 1,323,606 - Pf6N2E2_1127 0.56 -0.2 1,323,606 - Pf6N2E2_1127 0.56 +0.3 1,323,606 - Pf6N2E2_1127 0.56 +1.0 1,323,671 + Pf6N2E2_1127 0.59 +0.5 1,323,672 - Pf6N2E2_1127 0.59 +1.3 1,323,672 - Pf6N2E2_1127 0.59 -0.8 1,323,714 - Pf6N2E2_1127 0.60 -0.0 1,323,756 - Pf6N2E2_1127 0.62 +0.4 1,323,899 - Pf6N2E2_1127 0.68 -0.1 1,324,362 - Pf6N2E2_1127 0.88 -0.6 1,324,362 - Pf6N2E2_1127 0.88 +0.9 1,324,362 - Pf6N2E2_1127 0.88 -0.7 1,324,397 + Pf6N2E2_1127 0.90 +0.3 1,324,405 + +0.3 1,324,405 + +1.9 1,324,405 + -0.2 1,324,406 - +0.6 1,324,435 - +0.0 1,324,516 - +0.6 1,324,517 + +0.9 1,324,517 + +1.4 1,324,517 + -1.1 1,324,518 - +0.3 1,324,518 - +0.1 1,324,518 - -1.1 1,324,518 - +0.6 1,324,518 - +0.9 1,324,726 - Pf6N2E2_1128 0.15 -0.6 1,324,982 - Pf6N2E2_1128 0.54 -0.8 1,325,231 + -0.4
Or see this region's nucleotide sequence