Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1125

Experiment: Cephalothin 4 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1123 and Pf6N2E2_1124 overlap by 4 nucleotidesPf6N2E2_1124 and Pf6N2E2_1125 are separated by 142 nucleotidesPf6N2E2_1125 and Pf6N2E2_1126 are separated by 32 nucleotidesPf6N2E2_1126 and Pf6N2E2_1127 overlap by 4 nucleotides Pf6N2E2_1123 - Tol biopolymer transport system, TolR protein, at 1,319,520 to 1,319,966 _1123 Pf6N2E2_1124 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,319,963 to 1,320,778 _1124 Pf6N2E2_1125 - Transcriptional regulator, AraC family, at 1,320,921 to 1,321,817 _1125 Pf6N2E2_1126 - Two-component system regulatory protein, at 1,321,850 to 1,322,317 _1126 Pf6N2E2_1127 - Sensory box histidine kinase/response regulator, at 1,322,314 to 1,324,632 _1127 Position (kb) 1320 1321 1322Strain fitness (log2 ratio) -2 -1 0 1 2at 1320.207 kb on + strand, within Pf6N2E2_1124at 1320.599 kb on + strand, within Pf6N2E2_1124at 1320.600 kb on - strand, within Pf6N2E2_1124at 1320.600 kb on - strand, within Pf6N2E2_1124at 1320.754 kb on + strandat 1320.755 kb on - strandat 1320.789 kb on + strandat 1320.895 kb on - strandat 1320.922 kb on + strandat 1320.922 kb on + strandat 1320.922 kb on + strandat 1320.923 kb on - strandat 1321.081 kb on + strand, within Pf6N2E2_1125at 1321.081 kb on + strand, within Pf6N2E2_1125at 1321.082 kb on - strand, within Pf6N2E2_1125at 1321.082 kb on - strand, within Pf6N2E2_1125at 1321.132 kb on + strand, within Pf6N2E2_1125at 1321.143 kb on - strand, within Pf6N2E2_1125at 1321.241 kb on + strand, within Pf6N2E2_1125at 1321.242 kb on - strand, within Pf6N2E2_1125at 1321.300 kb on - strand, within Pf6N2E2_1125at 1321.484 kb on - strand, within Pf6N2E2_1125at 1321.537 kb on + strand, within Pf6N2E2_1125at 1321.538 kb on - strand, within Pf6N2E2_1125at 1321.927 kb on + strand, within Pf6N2E2_1126at 1321.927 kb on + strand, within Pf6N2E2_1126at 1321.929 kb on + strand, within Pf6N2E2_1126at 1321.929 kb on + strand, within Pf6N2E2_1126at 1321.929 kb on + strand, within Pf6N2E2_1126at 1321.929 kb on + strand, within Pf6N2E2_1126at 1321.930 kb on - strand, within Pf6N2E2_1126at 1321.930 kb on - strand, within Pf6N2E2_1126at 1321.930 kb on - strand, within Pf6N2E2_1126at 1322.080 kb on - strand, within Pf6N2E2_1126at 1322.330 kb on - strandat 1322.333 kb on + strandat 1322.333 kb on + strandat 1322.334 kb on - strandat 1322.365 kb on - strandat 1322.370 kb on + strandat 1322.370 kb on + strandat 1322.370 kb on + strandat 1322.370 kb on + strandat 1322.371 kb on - strandat 1322.371 kb on - strandat 1322.371 kb on - strandat 1322.371 kb on - strandat 1322.371 kb on - strandat 1322.749 kb on + strand, within Pf6N2E2_1127at 1322.749 kb on + strand, within Pf6N2E2_1127at 1322.749 kb on + strand, within Pf6N2E2_1127

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 4 mg/ml
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1,320,207 + Pf6N2E2_1124 0.30 +1.8
1,320,599 + Pf6N2E2_1124 0.78 +1.8
1,320,600 - Pf6N2E2_1124 0.78 -0.5
1,320,600 - Pf6N2E2_1124 0.78 +0.8
1,320,754 + +0.9
1,320,755 - +1.3
1,320,789 + -0.5
1,320,895 - +0.4
1,320,922 + -0.2
1,320,922 + +0.3
1,320,922 + +0.5
1,320,923 - +0.6
1,321,081 + Pf6N2E2_1125 0.18 +1.5
1,321,081 + Pf6N2E2_1125 0.18 +0.3
1,321,082 - Pf6N2E2_1125 0.18 +0.5
1,321,082 - Pf6N2E2_1125 0.18 +1.1
1,321,132 + Pf6N2E2_1125 0.24 +0.8
1,321,143 - Pf6N2E2_1125 0.25 +0.7
1,321,241 + Pf6N2E2_1125 0.36 +1.9
1,321,242 - Pf6N2E2_1125 0.36 -0.0
1,321,300 - Pf6N2E2_1125 0.42 -0.8
1,321,484 - Pf6N2E2_1125 0.63 +1.1
1,321,537 + Pf6N2E2_1125 0.69 +0.8
1,321,538 - Pf6N2E2_1125 0.69 +0.0
1,321,927 + Pf6N2E2_1126 0.16 -0.8
1,321,927 + Pf6N2E2_1126 0.16 +0.3
1,321,929 + Pf6N2E2_1126 0.17 -0.5
1,321,929 + Pf6N2E2_1126 0.17 +2.3
1,321,929 + Pf6N2E2_1126 0.17 +0.4
1,321,929 + Pf6N2E2_1126 0.17 +1.2
1,321,930 - Pf6N2E2_1126 0.17 -1.4
1,321,930 - Pf6N2E2_1126 0.17 +0.3
1,321,930 - Pf6N2E2_1126 0.17 +0.3
1,322,080 - Pf6N2E2_1126 0.49 +1.0
1,322,330 - +0.0
1,322,333 + +0.0
1,322,333 + +0.0
1,322,334 - +0.8
1,322,365 - -0.8
1,322,370 + -0.0
1,322,370 + -0.0
1,322,370 + +0.6
1,322,370 + -1.8
1,322,371 - +1.2
1,322,371 - -0.2
1,322,371 - -0.2
1,322,371 - +0.2
1,322,371 - -0.5
1,322,749 + Pf6N2E2_1127 0.19 -0.3
1,322,749 + Pf6N2E2_1127 0.19 -0.5
1,322,749 + Pf6N2E2_1127 0.19 +1.0

Or see this region's nucleotide sequence