Experiment: Cephalothin 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1123 and Pf6N2E2_1124 overlap by 4 nucleotides Pf6N2E2_1124 and Pf6N2E2_1125 are separated by 142 nucleotides Pf6N2E2_1125 and Pf6N2E2_1126 are separated by 32 nucleotides Pf6N2E2_1126 and Pf6N2E2_1127 overlap by 4 nucleotides
Pf6N2E2_1123 - Tol biopolymer transport system, TolR protein, at 1,319,520 to 1,319,966
_1123
Pf6N2E2_1124 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,319,963 to 1,320,778
_1124
Pf6N2E2_1125 - Transcriptional regulator, AraC family, at 1,320,921 to 1,321,817
_1125
Pf6N2E2_1126 - Two-component system regulatory protein, at 1,321,850 to 1,322,317
_1126
Pf6N2E2_1127 - Sensory box histidine kinase/response regulator, at 1,322,314 to 1,324,632
_1127
Position (kb)
1320
1321
1322 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1320.207 kb on + strand, within Pf6N2E2_1124 at 1320.599 kb on + strand, within Pf6N2E2_1124 at 1320.600 kb on - strand, within Pf6N2E2_1124 at 1320.600 kb on - strand, within Pf6N2E2_1124 at 1320.754 kb on + strand at 1320.755 kb on - strand at 1320.789 kb on + strand at 1320.895 kb on - strand at 1320.922 kb on + strand at 1320.922 kb on + strand at 1320.922 kb on + strand at 1320.923 kb on - strand at 1321.081 kb on + strand, within Pf6N2E2_1125 at 1321.081 kb on + strand, within Pf6N2E2_1125 at 1321.082 kb on - strand, within Pf6N2E2_1125 at 1321.082 kb on - strand, within Pf6N2E2_1125 at 1321.132 kb on + strand, within Pf6N2E2_1125 at 1321.143 kb on - strand, within Pf6N2E2_1125 at 1321.241 kb on + strand, within Pf6N2E2_1125 at 1321.242 kb on - strand, within Pf6N2E2_1125 at 1321.300 kb on - strand, within Pf6N2E2_1125 at 1321.484 kb on - strand, within Pf6N2E2_1125 at 1321.537 kb on + strand, within Pf6N2E2_1125 at 1321.538 kb on - strand, within Pf6N2E2_1125 at 1321.927 kb on + strand, within Pf6N2E2_1126 at 1321.927 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.929 kb on + strand, within Pf6N2E2_1126 at 1321.930 kb on - strand, within Pf6N2E2_1126 at 1321.930 kb on - strand, within Pf6N2E2_1126 at 1321.930 kb on - strand, within Pf6N2E2_1126 at 1322.080 kb on - strand, within Pf6N2E2_1126 at 1322.330 kb on - strand at 1322.333 kb on + strand at 1322.333 kb on + strand at 1322.334 kb on - strand at 1322.365 kb on - strand at 1322.370 kb on + strand at 1322.370 kb on + strand at 1322.370 kb on + strand at 1322.370 kb on + strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.371 kb on - strand at 1322.749 kb on + strand, within Pf6N2E2_1127 at 1322.749 kb on + strand, within Pf6N2E2_1127 at 1322.749 kb on + strand, within Pf6N2E2_1127
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 4 mg/ml remove 1,320,207 + Pf6N2E2_1124 0.30 +1.8 1,320,599 + Pf6N2E2_1124 0.78 +1.8 1,320,600 - Pf6N2E2_1124 0.78 -0.5 1,320,600 - Pf6N2E2_1124 0.78 +0.8 1,320,754 + +0.9 1,320,755 - +1.3 1,320,789 + -0.5 1,320,895 - +0.4 1,320,922 + -0.2 1,320,922 + +0.3 1,320,922 + +0.5 1,320,923 - +0.6 1,321,081 + Pf6N2E2_1125 0.18 +1.5 1,321,081 + Pf6N2E2_1125 0.18 +0.3 1,321,082 - Pf6N2E2_1125 0.18 +0.5 1,321,082 - Pf6N2E2_1125 0.18 +1.1 1,321,132 + Pf6N2E2_1125 0.24 +0.8 1,321,143 - Pf6N2E2_1125 0.25 +0.7 1,321,241 + Pf6N2E2_1125 0.36 +1.9 1,321,242 - Pf6N2E2_1125 0.36 -0.0 1,321,300 - Pf6N2E2_1125 0.42 -0.8 1,321,484 - Pf6N2E2_1125 0.63 +1.1 1,321,537 + Pf6N2E2_1125 0.69 +0.8 1,321,538 - Pf6N2E2_1125 0.69 +0.0 1,321,927 + Pf6N2E2_1126 0.16 -0.8 1,321,927 + Pf6N2E2_1126 0.16 +0.3 1,321,929 + Pf6N2E2_1126 0.17 -0.5 1,321,929 + Pf6N2E2_1126 0.17 +2.3 1,321,929 + Pf6N2E2_1126 0.17 +0.4 1,321,929 + Pf6N2E2_1126 0.17 +1.2 1,321,930 - Pf6N2E2_1126 0.17 -1.4 1,321,930 - Pf6N2E2_1126 0.17 +0.3 1,321,930 - Pf6N2E2_1126 0.17 +0.3 1,322,080 - Pf6N2E2_1126 0.49 +1.0 1,322,330 - +0.0 1,322,333 + +0.0 1,322,333 + +0.0 1,322,334 - +0.8 1,322,365 - -0.8 1,322,370 + -0.0 1,322,370 + -0.0 1,322,370 + +0.6 1,322,370 + -1.8 1,322,371 - +1.2 1,322,371 - -0.2 1,322,371 - -0.2 1,322,371 - +0.2 1,322,371 - -0.5 1,322,749 + Pf6N2E2_1127 0.19 -0.3 1,322,749 + Pf6N2E2_1127 0.19 -0.5 1,322,749 + Pf6N2E2_1127 0.19 +1.0
Or see this region's nucleotide sequence