Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05522 and H281DRAFT_05523 are separated by 41 nucleotides H281DRAFT_05523 and H281DRAFT_05524 are separated by 38 nucleotides
H281DRAFT_05522: H281DRAFT_05522 - 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase, at 104,029 to 105,603
_05522
H281DRAFT_05523: H281DRAFT_05523 - propionyl-CoA synthetase (EC 6.2.1.17), at 105,645 to 107,591
_05523
H281DRAFT_05524: H281DRAFT_05524 - Amidases related to nicotinamidase, at 107,630 to 108,103
_05524
Position (kb)
105
106
107
108 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 104.706 kb on + strand, within H281DRAFT_05522 at 104.707 kb on - strand, within H281DRAFT_05522 at 104.767 kb on + strand, within H281DRAFT_05522 at 104.768 kb on - strand, within H281DRAFT_05522 at 104.830 kb on + strand, within H281DRAFT_05522 at 104.830 kb on + strand, within H281DRAFT_05522 at 104.858 kb on - strand, within H281DRAFT_05522 at 104.858 kb on - strand, within H281DRAFT_05522 at 104.859 kb on + strand, within H281DRAFT_05522 at 104.859 kb on + strand, within H281DRAFT_05522 at 104.860 kb on - strand, within H281DRAFT_05522 at 104.867 kb on - strand, within H281DRAFT_05522 at 104.872 kb on + strand, within H281DRAFT_05522 at 104.872 kb on + strand, within H281DRAFT_05522 at 104.873 kb on - strand, within H281DRAFT_05522 at 104.892 kb on + strand, within H281DRAFT_05522 at 105.015 kb on + strand, within H281DRAFT_05522 at 105.038 kb on - strand, within H281DRAFT_05522 at 105.175 kb on + strand, within H281DRAFT_05522 at 105.175 kb on + strand, within H281DRAFT_05522 at 105.179 kb on + strand, within H281DRAFT_05522 at 105.258 kb on - strand, within H281DRAFT_05522 at 105.334 kb on + strand, within H281DRAFT_05522 at 105.501 kb on + strand at 105.535 kb on - strand at 105.807 kb on + strand at 105.903 kb on + strand, within H281DRAFT_05523 at 105.903 kb on + strand, within H281DRAFT_05523 at 105.904 kb on - strand, within H281DRAFT_05523 at 105.987 kb on + strand, within H281DRAFT_05523 at 105.987 kb on + strand, within H281DRAFT_05523 at 105.987 kb on + strand, within H281DRAFT_05523 at 105.988 kb on - strand, within H281DRAFT_05523 at 105.988 kb on - strand, within H281DRAFT_05523 at 105.990 kb on - strand, within H281DRAFT_05523 at 106.000 kb on + strand, within H281DRAFT_05523 at 106.000 kb on + strand, within H281DRAFT_05523 at 106.024 kb on + strand, within H281DRAFT_05523 at 106.025 kb on - strand at 106.221 kb on - strand, within H281DRAFT_05523 at 106.300 kb on - strand, within H281DRAFT_05523 at 106.300 kb on - strand, within H281DRAFT_05523 at 106.300 kb on - strand, within H281DRAFT_05523 at 106.314 kb on - strand, within H281DRAFT_05523 at 106.341 kb on + strand, within H281DRAFT_05523 at 106.341 kb on + strand, within H281DRAFT_05523 at 106.342 kb on - strand, within H281DRAFT_05523 at 106.352 kb on + strand, within H281DRAFT_05523 at 106.352 kb on + strand, within H281DRAFT_05523 at 106.352 kb on + strand, within H281DRAFT_05523 at 106.404 kb on + strand, within H281DRAFT_05523 at 106.405 kb on - strand, within H281DRAFT_05523 at 106.405 kb on - strand, within H281DRAFT_05523 at 106.405 kb on - strand, within H281DRAFT_05523 at 106.420 kb on + strand, within H281DRAFT_05523 at 106.421 kb on - strand, within H281DRAFT_05523 at 106.440 kb on + strand, within H281DRAFT_05523 at 106.440 kb on + strand, within H281DRAFT_05523 at 106.515 kb on + strand, within H281DRAFT_05523 at 106.539 kb on - strand, within H281DRAFT_05523 at 106.945 kb on + strand, within H281DRAFT_05523 at 106.986 kb on - strand, within H281DRAFT_05523 at 107.052 kb on + strand, within H281DRAFT_05523 at 107.089 kb on - strand, within H281DRAFT_05523 at 107.398 kb on + strand at 107.450 kb on - strand at 107.481 kb on - strand at 107.521 kb on + strand at 107.705 kb on + strand, within H281DRAFT_05524 at 107.759 kb on - strand, within H281DRAFT_05524 at 107.840 kb on + strand, within H281DRAFT_05524 at 107.884 kb on + strand, within H281DRAFT_05524 at 108.035 kb on + strand, within H281DRAFT_05524 at 108.035 kb on + strand, within H281DRAFT_05524 at 108.103 kb on + strand at 108.104 kb on - strand at 108.104 kb on - strand at 108.155 kb on + strand at 108.156 kb on - strand at 108.156 kb on - strand at 108.237 kb on + strand at 108.238 kb on - strand at 108.238 kb on - strand at 108.238 kb on - strand at 108.290 kb on - strand at 108.394 kb on - strand at 108.398 kb on - strand at 108.398 kb on - strand at 108.432 kb on + strand at 108.433 kb on - strand at 108.471 kb on + strand at 108.472 kb on - strand at 108.472 kb on - strand at 108.541 kb on + strand at 108.542 kb on - strand at 108.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM remove 104,706 + H281DRAFT_05522 0.43 +0.9 104,707 - H281DRAFT_05522 0.43 -1.0 104,767 + H281DRAFT_05522 0.47 -1.1 104,768 - H281DRAFT_05522 0.47 +0.1 104,830 + H281DRAFT_05522 0.51 -0.2 104,830 + H281DRAFT_05522 0.51 -0.2 104,858 - H281DRAFT_05522 0.53 -0.0 104,858 - H281DRAFT_05522 0.53 +0.0 104,859 + H281DRAFT_05522 0.53 -0.1 104,859 + H281DRAFT_05522 0.53 -0.0 104,860 - H281DRAFT_05522 0.53 +0.4 104,867 - H281DRAFT_05522 0.53 +0.8 104,872 + H281DRAFT_05522 0.54 -0.4 104,872 + H281DRAFT_05522 0.54 +0.4 104,873 - H281DRAFT_05522 0.54 +0.0 104,892 + H281DRAFT_05522 0.55 -1.7 105,015 + H281DRAFT_05522 0.63 +0.2 105,038 - H281DRAFT_05522 0.64 -0.9 105,175 + H281DRAFT_05522 0.73 +0.2 105,175 + H281DRAFT_05522 0.73 +0.1 105,179 + H281DRAFT_05522 0.73 -0.0 105,258 - H281DRAFT_05522 0.78 -0.5 105,334 + H281DRAFT_05522 0.83 +0.9 105,501 + -1.2 105,535 - -1.0 105,807 + +3.2 105,903 + H281DRAFT_05523 0.13 +0.2 105,903 + H281DRAFT_05523 0.13 -0.8 105,904 - H281DRAFT_05523 0.13 -0.2 105,987 + H281DRAFT_05523 0.18 +0.1 105,987 + H281DRAFT_05523 0.18 -0.0 105,987 + H281DRAFT_05523 0.18 +0.7 105,988 - H281DRAFT_05523 0.18 -0.6 105,988 - H281DRAFT_05523 0.18 +0.2 105,990 - H281DRAFT_05523 0.18 +0.5 106,000 + H281DRAFT_05523 0.18 -0.8 106,000 + H281DRAFT_05523 0.18 +0.2 106,024 + H281DRAFT_05523 0.19 -0.6 106,025 - -0.9 106,221 - H281DRAFT_05523 0.30 +0.2 106,300 - H281DRAFT_05523 0.34 -0.0 106,300 - H281DRAFT_05523 0.34 -0.6 106,300 - H281DRAFT_05523 0.34 -0.2 106,314 - H281DRAFT_05523 0.34 -0.0 106,341 + H281DRAFT_05523 0.36 -0.1 106,341 + H281DRAFT_05523 0.36 -0.3 106,342 - H281DRAFT_05523 0.36 -1.0 106,352 + H281DRAFT_05523 0.36 -0.2 106,352 + H281DRAFT_05523 0.36 -0.1 106,352 + H281DRAFT_05523 0.36 -0.5 106,404 + H281DRAFT_05523 0.39 +1.5 106,405 - H281DRAFT_05523 0.39 -0.5 106,405 - H281DRAFT_05523 0.39 -0.7 106,405 - H281DRAFT_05523 0.39 +0.1 106,420 + H281DRAFT_05523 0.40 +0.0 106,421 - H281DRAFT_05523 0.40 -1.5 106,440 + H281DRAFT_05523 0.41 -0.4 106,440 + H281DRAFT_05523 0.41 +0.6 106,515 + H281DRAFT_05523 0.45 +0.2 106,539 - H281DRAFT_05523 0.46 +0.2 106,945 + H281DRAFT_05523 0.67 -2.9 106,986 - H281DRAFT_05523 0.69 +0.1 107,052 + H281DRAFT_05523 0.72 +0.4 107,089 - H281DRAFT_05523 0.74 -0.2 107,398 + +0.3 107,450 - +0.1 107,481 - -0.0 107,521 + +0.4 107,705 + H281DRAFT_05524 0.16 -1.0 107,759 - H281DRAFT_05524 0.27 +0.7 107,840 + H281DRAFT_05524 0.44 +0.1 107,884 + H281DRAFT_05524 0.54 +0.7 108,035 + H281DRAFT_05524 0.85 -0.3 108,035 + H281DRAFT_05524 0.85 +0.1 108,103 + -0.4 108,104 - -1.2 108,104 - -1.1 108,155 + -0.6 108,156 - +0.5 108,156 - +0.5 108,237 + -0.3 108,238 - +0.6 108,238 - -0.3 108,238 - +0.5 108,290 - +0.8 108,394 - +0.1 108,398 - -0.1 108,398 - +1.2 108,432 + +0.3 108,433 - +0.6 108,471 + +0.7 108,472 - +0.1 108,472 - +0.7 108,541 + +1.4 108,542 - +0.4 108,542 - +2.4
Or see this region's nucleotide sequence