Experiment: Bacitracin 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_723 and Ac3H11_724 are separated by 79 nucleotides Ac3H11_724 and Ac3H11_725 are separated by 133 nucleotides Ac3H11_725 and Ac3H11_726 overlap by 4 nucleotides Ac3H11_726 and Ac3H11_727 overlap by 1 nucleotides
Ac3H11_723 - Cell division protein FtsN, at 13,445 to 14,107
_723
Ac3H11_724 - Periplasmic thiol:disulfide interchange protein DsbA, at 14,187 to 14,834
_724
Ac3H11_725 - LptA, protein essential for LPS transport across the periplasm, at 14,968 to 15,624
_725
Ac3H11_726 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 15,621 to 16,403
_726
Ac3H11_727 - RNA polymerase sigma-54 factor RpoN, at 16,403 to 17,986
_727
Position (kb)
14
15
16 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 14.171 kb on + strand at 14.171 kb on + strand at 14.171 kb on + strand at 14.171 kb on + strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.941 kb on + strand at 14.941 kb on + strand at 14.941 kb on + strand at 14.941 kb on + strand at 14.942 kb on - strand at 14.942 kb on - strand at 14.942 kb on - strand at 14.942 kb on - strand at 16.619 kb on + strand at 16.619 kb on + strand, within Ac3H11_727
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.032 mg/ml remove 14,171 + -0.6 14,171 + +2.0 14,171 + +0.5 14,171 + -2.3 14,833 - +0.2 14,833 - +0.1 14,833 - -0.3 14,833 - +0.5 14,833 - +0.3 14,941 + +2.8 14,941 + +0.3 14,941 + +0.6 14,941 + -0.3 14,942 - -0.9 14,942 - +0.1 14,942 - +0.8 14,942 - +1.7 16,619 + +0.7 16,619 + Ac3H11_727 0.14 +0.7
Or see this region's nucleotide sequence