Experiment: Bacitracin 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2426 and Ac3H11_2427 are separated by 178 nucleotides Ac3H11_2427 and Ac3H11_2428 are separated by 77 nucleotides Ac3H11_2428 and Ac3H11_2429 are separated by 223 nucleotides
Ac3H11_2426 - Enoyl-CoA hydratase (EC 4.2.1.17), at 684,484 to 685,329
_2426
Ac3H11_2427 - Threonine dehydratase, catabolic (EC 4.3.1.19), at 685,508 to 686,512
_2427
Ac3H11_2428 - Uracil phosphoribosyltransferase (EC 2.4.2.9), at 686,590 to 687,219
_2428
Ac3H11_2429 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 687,443 to 688,453
_2429
Position (kb)
685
686
687 Strain fitness (log2 ratio)
-2
-1
0
1 at 684.522 kb on + strand at 684.523 kb on - strand at 684.880 kb on + strand, within Ac3H11_2426 at 684.881 kb on - strand, within Ac3H11_2426 at 685.000 kb on + strand, within Ac3H11_2426 at 685.001 kb on - strand, within Ac3H11_2426 at 685.001 kb on - strand, within Ac3H11_2426 at 685.162 kb on + strand, within Ac3H11_2426 at 685.707 kb on - strand, within Ac3H11_2427 at 685.877 kb on + strand, within Ac3H11_2427 at 685.898 kb on + strand, within Ac3H11_2427 at 685.898 kb on + strand, within Ac3H11_2427 at 685.898 kb on + strand, within Ac3H11_2427 at 685.898 kb on + strand, within Ac3H11_2427 at 685.898 kb on + strand, within Ac3H11_2427 at 685.898 kb on + strand, within Ac3H11_2427 at 685.898 kb on + strand, within Ac3H11_2427 at 685.899 kb on - strand, within Ac3H11_2427 at 685.899 kb on - strand, within Ac3H11_2427 at 685.899 kb on - strand, within Ac3H11_2427 at 685.899 kb on - strand, within Ac3H11_2427 at 686.051 kb on + strand, within Ac3H11_2427 at 686.051 kb on + strand, within Ac3H11_2427 at 686.052 kb on - strand, within Ac3H11_2427 at 686.261 kb on + strand, within Ac3H11_2427 at 686.261 kb on + strand, within Ac3H11_2427 at 686.262 kb on - strand, within Ac3H11_2427 at 686.510 kb on + strand at 686.510 kb on + strand at 686.511 kb on - strand at 686.511 kb on - strand at 686.525 kb on + strand at 686.713 kb on + strand, within Ac3H11_2428 at 686.713 kb on + strand, within Ac3H11_2428 at 686.713 kb on + strand, within Ac3H11_2428 at 686.714 kb on - strand, within Ac3H11_2428 at 686.929 kb on + strand, within Ac3H11_2428 at 686.929 kb on + strand, within Ac3H11_2428 at 686.929 kb on + strand, within Ac3H11_2428 at 686.929 kb on + strand, within Ac3H11_2428 at 686.929 kb on + strand, within Ac3H11_2428 at 686.930 kb on - strand, within Ac3H11_2428 at 686.930 kb on - strand, within Ac3H11_2428 at 686.932 kb on + strand, within Ac3H11_2428 at 686.933 kb on - strand, within Ac3H11_2428 at 686.933 kb on - strand, within Ac3H11_2428 at 687.039 kb on + strand, within Ac3H11_2428 at 687.166 kb on + strand at 687.167 kb on - strand at 687.167 kb on - strand at 687.167 kb on - strand at 687.167 kb on - strand at 687.167 kb on - strand at 687.217 kb on + strand at 687.217 kb on + strand at 687.217 kb on + strand at 687.218 kb on - strand at 687.218 kb on - strand at 687.375 kb on - strand at 687.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.032 mg/ml remove 684,522 + -0.3 684,523 - -1.8 684,880 + Ac3H11_2426 0.47 +0.3 684,881 - Ac3H11_2426 0.47 -1.0 685,000 + Ac3H11_2426 0.61 -1.4 685,001 - Ac3H11_2426 0.61 +0.9 685,001 - Ac3H11_2426 0.61 -1.3 685,162 + Ac3H11_2426 0.80 -0.4 685,707 - Ac3H11_2427 0.20 +0.6 685,877 + Ac3H11_2427 0.37 +0.6 685,898 + Ac3H11_2427 0.39 +1.1 685,898 + Ac3H11_2427 0.39 -0.3 685,898 + Ac3H11_2427 0.39 +0.2 685,898 + Ac3H11_2427 0.39 -0.2 685,898 + Ac3H11_2427 0.39 -0.8 685,898 + Ac3H11_2427 0.39 -0.2 685,898 + Ac3H11_2427 0.39 -1.2 685,899 - Ac3H11_2427 0.39 -0.1 685,899 - Ac3H11_2427 0.39 -0.3 685,899 - Ac3H11_2427 0.39 +0.1 685,899 - Ac3H11_2427 0.39 -0.8 686,051 + Ac3H11_2427 0.54 +0.8 686,051 + Ac3H11_2427 0.54 -0.2 686,052 - Ac3H11_2427 0.54 +0.2 686,261 + Ac3H11_2427 0.75 -0.5 686,261 + Ac3H11_2427 0.75 -1.6 686,262 - Ac3H11_2427 0.75 +0.3 686,510 + -1.0 686,510 + +1.3 686,511 - -1.2 686,511 - +0.2 686,525 + +1.5 686,713 + Ac3H11_2428 0.20 +1.2 686,713 + Ac3H11_2428 0.20 -0.4 686,713 + Ac3H11_2428 0.20 +0.8 686,714 - Ac3H11_2428 0.20 -0.0 686,929 + Ac3H11_2428 0.54 +0.5 686,929 + Ac3H11_2428 0.54 -1.1 686,929 + Ac3H11_2428 0.54 -1.7 686,929 + Ac3H11_2428 0.54 +1.0 686,929 + Ac3H11_2428 0.54 -0.3 686,930 - Ac3H11_2428 0.54 -0.2 686,930 - Ac3H11_2428 0.54 -1.6 686,932 + Ac3H11_2428 0.54 -1.1 686,933 - Ac3H11_2428 0.54 -1.4 686,933 - Ac3H11_2428 0.54 +0.1 687,039 + Ac3H11_2428 0.71 -0.0 687,166 + +0.0 687,167 - -0.6 687,167 - -0.6 687,167 - -1.2 687,167 - -0.0 687,167 - +0.3 687,217 + -0.4 687,217 + -0.8 687,217 + -1.8 687,218 - +1.0 687,218 - +0.1 687,375 - -0.3 687,375 - +0.1
Or see this region's nucleotide sequence