Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_14140 and AO353_14145 overlap by 8 nucleotides AO353_14145 and AO353_14150 overlap by 8 nucleotides AO353_14150 and AO353_14155 are separated by 133 nucleotides
AO353_14140: AO353_14140 - isoleucine--tRNA ligase, at 3,140,727 to 3,143,558
_14140
AO353_14145: AO353_14145 - signal peptidase II, at 3,143,551 to 3,144,063
_14145
AO353_14150: AO353_14150 - peptidylprolyl isomerase, at 3,144,056 to 3,144,508
_14150
AO353_14155: AO353_14155 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 3,144,642 to 3,145,589
_14155
Position (kb)
3144
3145 Strain fitness (log2 ratio)
-2
-1
0
1 at 3144.021 kb on + strand at 3144.021 kb on + strand at 3144.021 kb on + strand at 3144.021 kb on + strand at 3144.069 kb on + strand at 3144.154 kb on + strand, within AO353_14150 at 3144.253 kb on + strand, within AO353_14150 at 3144.253 kb on + strand, within AO353_14150 at 3144.480 kb on + strand at 3144.480 kb on + strand at 3144.506 kb on + strand at 3144.506 kb on + strand at 3144.506 kb on + strand at 3144.513 kb on + strand at 3144.610 kb on + strand at 3144.610 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 3,144,021 + +0.7 3,144,021 + -1.3 3,144,021 + -0.6 3,144,021 + -0.1 3,144,069 + +0.9 3,144,154 + AO353_14150 0.22 -2.3 3,144,253 + AO353_14150 0.43 -0.3 3,144,253 + AO353_14150 0.43 -1.3 3,144,480 + -0.1 3,144,480 + -0.4 3,144,506 + -1.7 3,144,506 + +1.0 3,144,506 + +0.1 3,144,513 + +0.3 3,144,610 + +1.3 3,144,610 + +1.0
Or see this region's nucleotide sequence