Experiment: Hyaluronic from Streptococcus equi
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0621 and BT0622 are separated by 22 nucleotides BT0622 and BT0623 are separated by 258 nucleotides BT0623 and BT0624 are separated by 232 nucleotides
BT0621: BT0621 - Na+-transporting NADH:ubiquinone oxidoreductase, Electron transport complex protein rnfE (NCBI ptt file), at 766,159 to 766,743
BT0621
BT0622: BT0622 - Na+-transporting NADH:ubiquinone oxidoreductase, Electron transport complex protein rnfA (NCBI ptt file), at 766,766 to 767,338
BT0622
BT0623: BT0623 - putative UDP-glucose 4-epimerase (NCBI ptt file), at 767,597 to 768,631
BT0623
BT0624: BT0624 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (NCBI ptt file), at 768,864 to 769,688
BT0624
Position (kb) 767
768
769 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 766.598 kb on + strand, within BT0621 at 766.620 kb on + strand, within BT0621 at 766.626 kb on - strand, within BT0621 at 766.700 kb on + strand at 766.723 kb on - strand at 766.774 kb on + strand at 766.782 kb on + strand at 766.978 kb on - strand, within BT0622 at 767.060 kb on + strand, within BT0622 at 767.166 kb on + strand, within BT0622 at 767.383 kb on + strand at 767.393 kb on + strand at 767.425 kb on + strand at 767.451 kb on + strand at 767.482 kb on + strand at 768.692 kb on + strand at 768.737 kb on + strand at 768.748 kb on + strand at 768.748 kb on + strand at 768.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hyaluronic from Streptococcus equi remove 766,598 + BT0621 0.75 -1.2 766,620 + BT0621 0.79 -2.3 766,626 - BT0621 0.80 -1.3 766,700 + -0.3 766,723 - -0.3 766,774 + -0.5 766,782 + -1.6 766,978 - BT0622 0.37 -0.5 767,060 + BT0622 0.51 +0.6 767,166 + BT0622 0.70 +0.2 767,383 + +0.4 767,393 + +0.2 767,425 + -2.9 767,451 + -1.2 767,482 + -0.8 768,692 + -0.0 768,737 + -0.2 768,748 + +0.7 768,748 + -0.5 768,866 - -2.0
Or see this region's nucleotide sequence