Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_822

Experiment: Vancomycin 0.24 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_821 and Pf6N2E2_822 are separated by 341 nucleotidesPf6N2E2_822 and Pf6N2E2_823 are separated by 297 nucleotides Pf6N2E2_821 - KaiC, at 955,360 to 956,862 _821 Pf6N2E2_822 - TPR domain protein, at 957,204 to 958,262 _822 Pf6N2E2_823 - Outer membrane protein OprN, at 958,560 to 959,981 _823 Position (kb) 957 958 959Strain fitness (log2 ratio) -3 -2 -1 0 1at 956.204 kb on + strand, within Pf6N2E2_821at 956.219 kb on + strand, within Pf6N2E2_821at 956.255 kb on + strand, within Pf6N2E2_821at 956.256 kb on - strand, within Pf6N2E2_821at 956.256 kb on - strand, within Pf6N2E2_821at 956.256 kb on - strand, within Pf6N2E2_821at 956.375 kb on + strand, within Pf6N2E2_821at 956.375 kb on + strand, within Pf6N2E2_821at 956.375 kb on + strand, within Pf6N2E2_821at 956.375 kb on + strand, within Pf6N2E2_821at 956.376 kb on - strand, within Pf6N2E2_821at 956.534 kb on + strand, within Pf6N2E2_821at 956.595 kb on + strand, within Pf6N2E2_821at 956.596 kb on - strand, within Pf6N2E2_821at 956.675 kb on + strand, within Pf6N2E2_821at 956.675 kb on + strand, within Pf6N2E2_821at 956.676 kb on - strand, within Pf6N2E2_821at 956.676 kb on - strand, within Pf6N2E2_821at 956.850 kb on + strandat 956.851 kb on - strandat 956.856 kb on + strandat 956.866 kb on + strandat 956.963 kb on + strandat 956.963 kb on + strandat 956.963 kb on + strandat 956.972 kb on - strandat 956.983 kb on + strandat 956.983 kb on + strandat 956.984 kb on - strandat 956.984 kb on - strandat 957.016 kb on - strandat 957.036 kb on + strandat 957.199 kb on + strandat 957.199 kb on + strandat 957.206 kb on - strandat 957.301 kb on + strandat 957.301 kb on + strandat 957.301 kb on + strandat 957.302 kb on - strandat 957.302 kb on - strandat 957.419 kb on - strand, within Pf6N2E2_822at 957.478 kb on + strand, within Pf6N2E2_822at 957.478 kb on + strand, within Pf6N2E2_822at 957.479 kb on - strand, within Pf6N2E2_822at 957.530 kb on - strand, within Pf6N2E2_822at 957.570 kb on + strand, within Pf6N2E2_822at 957.606 kb on + strand, within Pf6N2E2_822at 957.606 kb on + strand, within Pf6N2E2_822at 957.607 kb on - strand, within Pf6N2E2_822at 958.016 kb on - strand, within Pf6N2E2_822at 958.084 kb on + strand, within Pf6N2E2_822at 958.084 kb on + strand, within Pf6N2E2_822at 958.085 kb on - strand, within Pf6N2E2_822at 958.142 kb on - strand, within Pf6N2E2_822at 958.191 kb on + strandat 958.240 kb on + strandat 958.609 kb on + strandat 958.609 kb on + strandat 958.609 kb on + strandat 958.609 kb on + strandat 958.609 kb on + strandat 958.609 kb on + strandat 958.851 kb on + strand, within Pf6N2E2_823at 958.851 kb on + strand, within Pf6N2E2_823at 958.851 kb on + strand, within Pf6N2E2_823at 958.852 kb on - strand, within Pf6N2E2_823at 958.852 kb on - strand, within Pf6N2E2_823at 958.852 kb on - strand, within Pf6N2E2_823at 958.864 kb on + strand, within Pf6N2E2_823at 958.864 kb on + strand, within Pf6N2E2_823at 958.864 kb on + strand, within Pf6N2E2_823at 958.864 kb on + strand, within Pf6N2E2_823at 958.865 kb on - strand, within Pf6N2E2_823at 958.946 kb on + strand, within Pf6N2E2_823at 958.947 kb on - strand, within Pf6N2E2_823at 958.947 kb on - strandat 958.947 kb on - strand, within Pf6N2E2_823at 958.947 kb on - strand, within Pf6N2E2_823at 958.947 kb on - strand, within Pf6N2E2_823at 958.947 kb on - strand, within Pf6N2E2_823

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml
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956,204 + Pf6N2E2_821 0.56 +1.1
956,219 + Pf6N2E2_821 0.57 +0.2
956,255 + Pf6N2E2_821 0.60 -0.5
956,256 - Pf6N2E2_821 0.60 +0.5
956,256 - Pf6N2E2_821 0.60 -0.4
956,256 - Pf6N2E2_821 0.60 -0.7
956,375 + Pf6N2E2_821 0.68 +0.2
956,375 + Pf6N2E2_821 0.68 -0.1
956,375 + Pf6N2E2_821 0.68 -0.1
956,375 + Pf6N2E2_821 0.68 -1.1
956,376 - Pf6N2E2_821 0.68 -3.0
956,534 + Pf6N2E2_821 0.78 +0.5
956,595 + Pf6N2E2_821 0.82 +0.6
956,596 - Pf6N2E2_821 0.82 -0.5
956,675 + Pf6N2E2_821 0.87 -0.4
956,675 + Pf6N2E2_821 0.87 -1.7
956,676 - Pf6N2E2_821 0.88 -2.1
956,676 - Pf6N2E2_821 0.88 -0.1
956,850 + -1.1
956,851 - -0.6
956,856 + -0.2
956,866 + +0.9
956,963 + -0.7
956,963 + +0.5
956,963 + -2.6
956,972 - +0.2
956,983 + -0.3
956,983 + +0.6
956,984 - -1.1
956,984 - +0.6
957,016 - -0.5
957,036 + +0.7
957,199 + -0.3
957,199 + -1.4
957,206 - -1.7
957,301 + -2.2
957,301 + -0.9
957,301 + -0.3
957,302 - -0.3
957,302 - -1.1
957,419 - Pf6N2E2_822 0.20 -1.5
957,478 + Pf6N2E2_822 0.26 -0.4
957,478 + Pf6N2E2_822 0.26 -2.4
957,479 - Pf6N2E2_822 0.26 -3.4
957,530 - Pf6N2E2_822 0.31 -0.1
957,570 + Pf6N2E2_822 0.35 -1.1
957,606 + Pf6N2E2_822 0.38 -1.1
957,606 + Pf6N2E2_822 0.38 -0.7
957,607 - Pf6N2E2_822 0.38 -1.4
958,016 - Pf6N2E2_822 0.77 -2.7
958,084 + Pf6N2E2_822 0.83 -1.0
958,084 + Pf6N2E2_822 0.83 -1.7
958,085 - Pf6N2E2_822 0.83 -2.8
958,142 - Pf6N2E2_822 0.89 -1.4
958,191 + -1.1
958,240 + -1.9
958,609 + +0.9
958,609 + -0.2
958,609 + +0.3
958,609 + -0.3
958,609 + +1.5
958,609 + +1.4
958,851 + Pf6N2E2_823 0.20 +0.7
958,851 + Pf6N2E2_823 0.20 -0.3
958,851 + Pf6N2E2_823 0.20 -0.1
958,852 - Pf6N2E2_823 0.21 +0.4
958,852 - Pf6N2E2_823 0.21 -0.9
958,852 - Pf6N2E2_823 0.21 +0.3
958,864 + Pf6N2E2_823 0.21 +0.4
958,864 + Pf6N2E2_823 0.21 +0.3
958,864 + Pf6N2E2_823 0.21 +0.1
958,864 + Pf6N2E2_823 0.21 -0.8
958,865 - Pf6N2E2_823 0.21 +1.0
958,946 + Pf6N2E2_823 0.27 +0.4
958,947 - Pf6N2E2_823 0.27 -0.7
958,947 - -0.1
958,947 - Pf6N2E2_823 0.27 +0.5
958,947 - Pf6N2E2_823 0.27 +0.5
958,947 - Pf6N2E2_823 0.27 -0.5
958,947 - Pf6N2E2_823 0.27 +1.2

Or see this region's nucleotide sequence