Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_581

Experiment: Vancomycin 0.24 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_580 and Pf6N2E2_581 are separated by 19 nucleotidesPf6N2E2_581 and Pf6N2E2_582 are separated by 1 nucleotides Pf6N2E2_580 - Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9), at 655,017 to 655,811 _580 Pf6N2E2_581 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 655,831 to 657,441 _581 Pf6N2E2_582 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 657,443 to 658,462 _582 Position (kb) 655 656 657 658Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 654.865 kb on + strandat 654.866 kb on - strandat 654.873 kb on + strandat 654.873 kb on + strandat 654.873 kb on + strandat 654.873 kb on + strandat 654.873 kb on + strandat 654.873 kb on + strandat 654.873 kb on + strandat 654.925 kb on + strandat 654.925 kb on + strandat 654.926 kb on - strandat 654.936 kb on - strandat 654.936 kb on - strandat 654.936 kb on - strandat 655.018 kb on + strandat 655.882 kb on - strandat 655.882 kb on - strandat 655.882 kb on - strandat 655.882 kb on - strandat 655.882 kb on - strandat 655.882 kb on - strandat 655.882 kb on - strandat 655.882 kb on - strandat 655.882 kb on - strandat 655.884 kb on - strandat 655.884 kb on - strandat 656.010 kb on - strand, within Pf6N2E2_581at 656.010 kb on - strand, within Pf6N2E2_581at 656.010 kb on - strand, within Pf6N2E2_581at 656.022 kb on - strand, within Pf6N2E2_581at 656.022 kb on - strand, within Pf6N2E2_581at 656.022 kb on - strand, within Pf6N2E2_581at 656.071 kb on - strand, within Pf6N2E2_581at 656.210 kb on - strand, within Pf6N2E2_581at 656.300 kb on - strand, within Pf6N2E2_581at 656.300 kb on - strand, within Pf6N2E2_581at 656.300 kb on - strand, within Pf6N2E2_581at 656.550 kb on - strand, within Pf6N2E2_581at 656.550 kb on - strand, within Pf6N2E2_581at 656.550 kb on - strand, within Pf6N2E2_581at 656.550 kb on - strand, within Pf6N2E2_581at 656.550 kb on - strand, within Pf6N2E2_581at 656.550 kb on - strand, within Pf6N2E2_581at 656.550 kb on - strand, within Pf6N2E2_581at 656.758 kb on + strand, within Pf6N2E2_581at 656.758 kb on + strand, within Pf6N2E2_581at 656.758 kb on + strand, within Pf6N2E2_581at 656.758 kb on + strand, within Pf6N2E2_581at 656.758 kb on + strand, within Pf6N2E2_581at 656.758 kb on + strand, within Pf6N2E2_581at 656.758 kb on + strand, within Pf6N2E2_581at 656.759 kb on - strand, within Pf6N2E2_581at 656.759 kb on - strand, within Pf6N2E2_581at 656.759 kb on - strand, within Pf6N2E2_581at 656.759 kb on - strand, within Pf6N2E2_581at 657.221 kb on + strand, within Pf6N2E2_581at 657.221 kb on + strand, within Pf6N2E2_581at 657.222 kb on - strand, within Pf6N2E2_581at 657.430 kb on - strandat 657.762 kb on + strand, within Pf6N2E2_582at 657.763 kb on - strand, within Pf6N2E2_582at 657.894 kb on + strand, within Pf6N2E2_582at 657.960 kb on + strand, within Pf6N2E2_582at 657.960 kb on + strand, within Pf6N2E2_582at 657.961 kb on - strand, within Pf6N2E2_582at 658.028 kb on + strand, within Pf6N2E2_582at 658.029 kb on - strand, within Pf6N2E2_582at 658.029 kb on - strand, within Pf6N2E2_582at 658.029 kb on - strand, within Pf6N2E2_582at 658.029 kb on - strand, within Pf6N2E2_582at 658.050 kb on + strand, within Pf6N2E2_582at 658.050 kb on + strand, within Pf6N2E2_582at 658.050 kb on + strand, within Pf6N2E2_582at 658.051 kb on - strand, within Pf6N2E2_582at 658.051 kb on - strand, within Pf6N2E2_582at 658.144 kb on - strand, within Pf6N2E2_582at 658.159 kb on - strand, within Pf6N2E2_582at 658.159 kb on - strand, within Pf6N2E2_582at 658.159 kb on - strand, within Pf6N2E2_582at 658.221 kb on + strand, within Pf6N2E2_582at 658.221 kb on + strand, within Pf6N2E2_582at 658.222 kb on - strand, within Pf6N2E2_582at 658.222 kb on - strand, within Pf6N2E2_582at 658.222 kb on - strand, within Pf6N2E2_582at 658.222 kb on - strand, within Pf6N2E2_582at 658.222 kb on - strand, within Pf6N2E2_582at 658.233 kb on + strand, within Pf6N2E2_582at 658.233 kb on + strand, within Pf6N2E2_582at 658.233 kb on + strand, within Pf6N2E2_582at 658.234 kb on - strand, within Pf6N2E2_582at 658.314 kb on + strand, within Pf6N2E2_582at 658.315 kb on - strand, within Pf6N2E2_582

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml
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654,865 + +1.2
654,866 - -1.5
654,873 + +1.1
654,873 + -0.1
654,873 + +0.4
654,873 + +0.4
654,873 + -0.0
654,873 + -0.4
654,873 + -1.1
654,925 + -0.9
654,925 + -0.8
654,926 - +0.6
654,936 - +0.5
654,936 - +0.1
654,936 - +0.6
655,018 + -0.7
655,882 - -2.5
655,882 - -0.4
655,882 - +0.4
655,882 - +1.0
655,882 - -0.1
655,882 - +0.2
655,882 - -1.4
655,882 - +1.0
655,882 - -0.1
655,884 - -1.1
655,884 - -1.5
656,010 - Pf6N2E2_581 0.11 -1.3
656,010 - Pf6N2E2_581 0.11 -0.7
656,010 - Pf6N2E2_581 0.11 -0.1
656,022 - Pf6N2E2_581 0.12 +0.1
656,022 - Pf6N2E2_581 0.12 -0.4
656,022 - Pf6N2E2_581 0.12 -2.0
656,071 - Pf6N2E2_581 0.15 -1.4
656,210 - Pf6N2E2_581 0.24 -1.2
656,300 - Pf6N2E2_581 0.29 +0.9
656,300 - Pf6N2E2_581 0.29 -1.1
656,300 - Pf6N2E2_581 0.29 -1.0
656,550 - Pf6N2E2_581 0.45 -2.1
656,550 - Pf6N2E2_581 0.45 +1.9
656,550 - Pf6N2E2_581 0.45 +0.1
656,550 - Pf6N2E2_581 0.45 +0.2
656,550 - Pf6N2E2_581 0.45 -0.5
656,550 - Pf6N2E2_581 0.45 -1.3
656,550 - Pf6N2E2_581 0.45 -1.6
656,758 + Pf6N2E2_581 0.58 -0.7
656,758 + Pf6N2E2_581 0.58 -1.8
656,758 + Pf6N2E2_581 0.58 -0.7
656,758 + Pf6N2E2_581 0.58 -0.3
656,758 + Pf6N2E2_581 0.58 -3.2
656,758 + Pf6N2E2_581 0.58 -0.0
656,758 + Pf6N2E2_581 0.58 -1.1
656,759 - Pf6N2E2_581 0.58 +0.0
656,759 - Pf6N2E2_581 0.58 -0.3
656,759 - Pf6N2E2_581 0.58 -1.8
656,759 - Pf6N2E2_581 0.58 -0.4
657,221 + Pf6N2E2_581 0.86 -0.6
657,221 + Pf6N2E2_581 0.86 -0.6
657,222 - Pf6N2E2_581 0.86 -0.5
657,430 - +0.5
657,762 + Pf6N2E2_582 0.31 -1.9
657,763 - Pf6N2E2_582 0.31 +0.6
657,894 + Pf6N2E2_582 0.44 -0.9
657,960 + Pf6N2E2_582 0.51 -0.5
657,960 + Pf6N2E2_582 0.51 -2.7
657,961 - Pf6N2E2_582 0.51 -0.9
658,028 + Pf6N2E2_582 0.57 -1.5
658,029 - Pf6N2E2_582 0.57 -3.3
658,029 - Pf6N2E2_582 0.57 -1.0
658,029 - Pf6N2E2_582 0.57 -2.1
658,029 - Pf6N2E2_582 0.57 -1.7
658,050 + Pf6N2E2_582 0.60 -0.5
658,050 + Pf6N2E2_582 0.60 +0.4
658,050 + Pf6N2E2_582 0.60 -0.8
658,051 - Pf6N2E2_582 0.60 -0.8
658,051 - Pf6N2E2_582 0.60 -0.1
658,144 - Pf6N2E2_582 0.69 +0.1
658,159 - Pf6N2E2_582 0.70 -1.0
658,159 - Pf6N2E2_582 0.70 +2.7
658,159 - Pf6N2E2_582 0.70 -1.2
658,221 + Pf6N2E2_582 0.76 -0.1
658,221 + Pf6N2E2_582 0.76 +0.3
658,222 - Pf6N2E2_582 0.76 -1.3
658,222 - Pf6N2E2_582 0.76 -0.9
658,222 - Pf6N2E2_582 0.76 -0.6
658,222 - Pf6N2E2_582 0.76 -0.1
658,222 - Pf6N2E2_582 0.76 -0.6
658,233 + Pf6N2E2_582 0.77 -2.5
658,233 + Pf6N2E2_582 0.77 -0.3
658,233 + Pf6N2E2_582 0.77 -3.5
658,234 - Pf6N2E2_582 0.78 -3.6
658,314 + Pf6N2E2_582 0.85 -1.7
658,315 - Pf6N2E2_582 0.85 -0.3

Or see this region's nucleotide sequence