Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5803 and Pf6N2E2_5804 are separated by 54 nucleotides Pf6N2E2_5804 and Pf6N2E2_5805 overlap by 4 nucleotides Pf6N2E2_5805 and Pf6N2E2_5806 are separated by 45 nucleotides
Pf6N2E2_5803 - Allantoicase (EC 3.5.3.4), at 6,620,661 to 6,621,656
_5803
Pf6N2E2_5804 - 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) decarboxylase, at 6,621,711 to 6,622,226
_5804
Pf6N2E2_5805 - Uricase (urate oxidase) (EC 1.7.3.3), at 6,622,223 to 6,623,164
_5805
Pf6N2E2_5806 - hypothetical protein, at 6,623,210 to 6,623,386
_5806
Position (kb)
6621
6622
6623 Strain fitness (log2 ratio)
-2
-1
0
1 at 6620.896 kb on + strand, within Pf6N2E2_5803 at 6620.897 kb on - strand, within Pf6N2E2_5803 at 6621.169 kb on + strand, within Pf6N2E2_5803 at 6621.202 kb on + strand, within Pf6N2E2_5803 at 6621.206 kb on + strand, within Pf6N2E2_5803 at 6621.206 kb on + strand, within Pf6N2E2_5803 at 6621.207 kb on - strand, within Pf6N2E2_5803 at 6621.367 kb on + strand, within Pf6N2E2_5803 at 6621.368 kb on - strand, within Pf6N2E2_5803 at 6621.368 kb on - strand, within Pf6N2E2_5803 at 6621.371 kb on + strand, within Pf6N2E2_5803 at 6621.372 kb on - strand, within Pf6N2E2_5803 at 6621.639 kb on + strand at 6621.646 kb on + strand at 6621.646 kb on + strand at 6621.647 kb on - strand at 6621.647 kb on - strand at 6621.647 kb on - strand at 6621.673 kb on + strand at 6621.673 kb on + strand at 6621.673 kb on + strand at 6621.674 kb on - strand at 6621.676 kb on + strand at 6621.676 kb on + strand at 6621.676 kb on + strand at 6621.676 kb on + strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.840 kb on + strand, within Pf6N2E2_5804 at 6621.877 kb on + strand, within Pf6N2E2_5804 at 6621.877 kb on + strand, within Pf6N2E2_5804 at 6621.877 kb on + strand, within Pf6N2E2_5804 at 6621.878 kb on - strand, within Pf6N2E2_5804 at 6622.153 kb on + strand, within Pf6N2E2_5804 at 6622.154 kb on - strand, within Pf6N2E2_5804 at 6622.257 kb on + strand at 6622.288 kb on + strand at 6622.332 kb on + strand, within Pf6N2E2_5805 at 6622.332 kb on + strand, within Pf6N2E2_5805 at 6622.333 kb on - strand, within Pf6N2E2_5805 at 6622.435 kb on - strand, within Pf6N2E2_5805 at 6622.496 kb on + strand, within Pf6N2E2_5805 at 6622.497 kb on - strand, within Pf6N2E2_5805 at 6622.497 kb on - strand, within Pf6N2E2_5805 at 6622.522 kb on + strand, within Pf6N2E2_5805 at 6622.582 kb on - strand, within Pf6N2E2_5805 at 6622.599 kb on + strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.615 kb on - strand, within Pf6N2E2_5805 at 6622.615 kb on - strand, within Pf6N2E2_5805 at 6622.674 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6623.218 kb on + strand at 6623.218 kb on + strand at 6623.218 kb on + strand at 6623.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 6,620,896 + Pf6N2E2_5803 0.24 -0.9 6,620,897 - Pf6N2E2_5803 0.24 -0.7 6,621,169 + Pf6N2E2_5803 0.51 +0.5 6,621,202 + Pf6N2E2_5803 0.54 -0.5 6,621,206 + Pf6N2E2_5803 0.55 -0.7 6,621,206 + Pf6N2E2_5803 0.55 -1.8 6,621,207 - Pf6N2E2_5803 0.55 +0.5 6,621,367 + Pf6N2E2_5803 0.71 +0.4 6,621,368 - Pf6N2E2_5803 0.71 +0.2 6,621,368 - Pf6N2E2_5803 0.71 +0.8 6,621,371 + Pf6N2E2_5803 0.71 +0.5 6,621,372 - Pf6N2E2_5803 0.71 -0.7 6,621,639 + +1.6 6,621,646 + +0.3 6,621,646 + -0.4 6,621,647 - -0.6 6,621,647 - +1.1 6,621,647 - +1.0 6,621,673 + +0.6 6,621,673 + +0.2 6,621,673 + +1.1 6,621,674 - +0.9 6,621,676 + -0.5 6,621,676 + +1.2 6,621,676 + -0.8 6,621,676 + -0.4 6,621,677 - -0.5 6,621,677 - +0.3 6,621,677 - +1.3 6,621,677 - -0.1 6,621,677 - -0.9 6,621,840 + Pf6N2E2_5804 0.25 -0.7 6,621,877 + Pf6N2E2_5804 0.32 -0.4 6,621,877 + Pf6N2E2_5804 0.32 -0.6 6,621,877 + Pf6N2E2_5804 0.32 -0.7 6,621,878 - Pf6N2E2_5804 0.32 -1.7 6,622,153 + Pf6N2E2_5804 0.86 -0.7 6,622,154 - Pf6N2E2_5804 0.86 -1.1 6,622,257 + +0.8 6,622,288 + -0.4 6,622,332 + Pf6N2E2_5805 0.12 +0.8 6,622,332 + Pf6N2E2_5805 0.12 +0.5 6,622,333 - Pf6N2E2_5805 0.12 -0.7 6,622,435 - Pf6N2E2_5805 0.23 -0.1 6,622,496 + Pf6N2E2_5805 0.29 -1.3 6,622,497 - Pf6N2E2_5805 0.29 +0.3 6,622,497 - Pf6N2E2_5805 0.29 +0.5 6,622,522 + Pf6N2E2_5805 0.32 -1.0 6,622,582 - Pf6N2E2_5805 0.38 -1.7 6,622,599 + Pf6N2E2_5805 0.40 +0.8 6,622,600 - Pf6N2E2_5805 0.40 +0.2 6,622,600 - Pf6N2E2_5805 0.40 -0.7 6,622,600 - Pf6N2E2_5805 0.40 -0.4 6,622,600 - Pf6N2E2_5805 0.40 -0.3 6,622,615 - Pf6N2E2_5805 0.42 +0.6 6,622,615 - Pf6N2E2_5805 0.42 +0.2 6,622,674 + Pf6N2E2_5805 0.48 -1.1 6,622,761 + Pf6N2E2_5805 0.57 -0.8 6,622,761 + Pf6N2E2_5805 0.57 -2.0 6,622,761 + Pf6N2E2_5805 0.57 -0.2 6,622,761 + Pf6N2E2_5805 0.57 -1.4 6,622,761 + Pf6N2E2_5805 0.57 +1.1 6,622,761 + Pf6N2E2_5805 0.57 -0.7 6,622,761 + Pf6N2E2_5805 0.57 -0.1 6,622,761 + Pf6N2E2_5805 0.57 +1.3 6,622,761 + Pf6N2E2_5805 0.57 +0.9 6,622,762 - Pf6N2E2_5805 0.57 +0.1 6,622,762 - Pf6N2E2_5805 0.57 -0.8 6,622,762 - Pf6N2E2_5805 0.57 -2.2 6,622,762 - Pf6N2E2_5805 0.57 +0.4 6,622,762 - Pf6N2E2_5805 0.57 -1.2 6,623,218 + -0.7 6,623,218 + +0.1 6,623,218 + +1.3 6,623,219 - +0.3
Or see this region's nucleotide sequence