Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5536

Experiment: Vancomycin 0.24 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5534 and Pf6N2E2_5535 are separated by 181 nucleotidesPf6N2E2_5535 and Pf6N2E2_5536 are separated by 113 nucleotidesPf6N2E2_5536 and Pf6N2E2_5537 are separated by 0 nucleotides Pf6N2E2_5534 - FIG000859: hypothetical protein YebC, at 6,350,944 to 6,351,690 _5534 Pf6N2E2_5535 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 6,351,872 to 6,352,396 _5535 Pf6N2E2_5536 - Holliday junction DNA helicase RuvA, at 6,352,510 to 6,353,118 _5536 Pf6N2E2_5537 - Holliday junction DNA helicase RuvB, at 6,353,119 to 6,354,180 _5537 Position (kb) 6352 6353 6354Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 6351.847 kb on - strandat 6351.855 kb on + strandat 6351.872 kb on - strandat 6351.879 kb on + strandat 6352.572 kb on - strand, within Pf6N2E2_5536at 6353.215 kb on + strandat 6354.052 kb on + strand, within Pf6N2E2_5537at 6354.113 kb on + strandat 6354.114 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml
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6,351,847 - -2.3
6,351,855 + -0.1
6,351,872 - -1.0
6,351,879 + -1.2
6,352,572 - Pf6N2E2_5536 0.10 -0.8
6,353,215 + -3.5
6,354,052 + Pf6N2E2_5537 0.88 -1.3
6,354,113 + +1.4
6,354,114 - -4.5

Or see this region's nucleotide sequence