Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5478 and Pf6N2E2_5479 overlap by 4 nucleotides Pf6N2E2_5479 and Pf6N2E2_5480 are separated by 15 nucleotides
Pf6N2E2_5478 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,296,331 to 6,297,197
_5478
Pf6N2E2_5479 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,297,194 to 6,298,042
_5479
Pf6N2E2_5480 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 6,298,058 to 6,299,113
_5480
Position (kb)
6297
6298
6299 Strain fitness (log2 ratio)
-2
-1
0
1 at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.349 kb on - strand at 6296.491 kb on - strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.514 kb on + strand, within Pf6N2E2_5478 at 6296.515 kb on - strand, within Pf6N2E2_5478 at 6296.515 kb on - strand, within Pf6N2E2_5478 at 6296.596 kb on - strand, within Pf6N2E2_5478 at 6296.596 kb on - strand, within Pf6N2E2_5478 at 6296.688 kb on + strand, within Pf6N2E2_5478 at 6296.688 kb on + strand, within Pf6N2E2_5478 at 6296.689 kb on - strand, within Pf6N2E2_5478 at 6296.689 kb on - strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.985 kb on + strand, within Pf6N2E2_5478 at 6296.986 kb on - strand, within Pf6N2E2_5478 at 6297.153 kb on + strand at 6297.360 kb on - strand, within Pf6N2E2_5479 at 6297.872 kb on + strand, within Pf6N2E2_5479 at 6297.872 kb on + strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.881 kb on + strand, within Pf6N2E2_5479 at 6297.881 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.918 kb on - strand, within Pf6N2E2_5479 at 6297.918 kb on - strand, within Pf6N2E2_5479 at 6298.071 kb on + strand at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.722 kb on - strand, within Pf6N2E2_5480 at 6299.026 kb on + strand at 6299.026 kb on + strand at 6299.027 kb on - strand at 6299.027 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 6,296,348 + +0.7 6,296,348 + -0.3 6,296,348 + +0.9 6,296,348 + -0.9 6,296,349 - +0.8 6,296,491 - Pf6N2E2_5478 0.18 +1.1 6,296,511 + Pf6N2E2_5478 0.21 -0.2 6,296,511 + Pf6N2E2_5478 0.21 +0.4 6,296,511 + Pf6N2E2_5478 0.21 +0.6 6,296,512 - Pf6N2E2_5478 0.21 -1.4 6,296,512 - Pf6N2E2_5478 0.21 -1.1 6,296,512 - Pf6N2E2_5478 0.21 -1.0 6,296,512 - Pf6N2E2_5478 0.21 +0.5 6,296,514 + Pf6N2E2_5478 0.21 -0.1 6,296,515 - Pf6N2E2_5478 0.21 +0.4 6,296,515 - Pf6N2E2_5478 0.21 -1.6 6,296,596 - Pf6N2E2_5478 0.31 -0.4 6,296,596 - Pf6N2E2_5478 0.31 +0.2 6,296,688 + Pf6N2E2_5478 0.41 -0.8 6,296,688 + Pf6N2E2_5478 0.41 -0.4 6,296,689 - Pf6N2E2_5478 0.41 -1.9 6,296,689 - Pf6N2E2_5478 0.41 -0.3 6,296,778 + Pf6N2E2_5478 0.52 +0.6 6,296,778 + Pf6N2E2_5478 0.52 -0.7 6,296,778 + Pf6N2E2_5478 0.52 -1.0 6,296,778 + Pf6N2E2_5478 0.52 -0.2 6,296,778 + Pf6N2E2_5478 0.52 -0.6 6,296,779 - Pf6N2E2_5478 0.52 -0.9 6,296,779 - Pf6N2E2_5478 0.52 +0.6 6,296,779 - Pf6N2E2_5478 0.52 +0.9 6,296,874 + Pf6N2E2_5478 0.63 -0.2 6,296,874 + Pf6N2E2_5478 0.63 +0.3 6,296,874 + Pf6N2E2_5478 0.63 +0.9 6,296,874 + Pf6N2E2_5478 0.63 +1.0 6,296,874 + Pf6N2E2_5478 0.63 +0.7 6,296,874 + Pf6N2E2_5478 0.63 +0.0 6,296,985 + Pf6N2E2_5478 0.75 -1.2 6,296,986 - Pf6N2E2_5478 0.76 +0.4 6,297,153 + +0.7 6,297,360 - Pf6N2E2_5479 0.20 +0.5 6,297,872 + Pf6N2E2_5479 0.80 +0.5 6,297,872 + Pf6N2E2_5479 0.80 +1.4 6,297,873 - Pf6N2E2_5479 0.80 -1.4 6,297,873 - Pf6N2E2_5479 0.80 -2.3 6,297,873 - Pf6N2E2_5479 0.80 +0.0 6,297,873 - Pf6N2E2_5479 0.80 +0.1 6,297,881 + Pf6N2E2_5479 0.81 -0.3 6,297,881 + Pf6N2E2_5479 0.81 -0.3 6,297,917 + Pf6N2E2_5479 0.85 +0.4 6,297,917 + Pf6N2E2_5479 0.85 +1.2 6,297,917 + Pf6N2E2_5479 0.85 +1.2 6,297,917 + Pf6N2E2_5479 0.85 +1.4 6,297,918 - Pf6N2E2_5479 0.85 +1.6 6,297,918 - Pf6N2E2_5479 0.85 +0.5 6,298,071 + -0.4 6,298,648 + Pf6N2E2_5480 0.56 -1.6 6,298,648 + Pf6N2E2_5480 0.56 -0.1 6,298,648 + Pf6N2E2_5480 0.56 +0.5 6,298,648 + Pf6N2E2_5480 0.56 -0.8 6,298,648 + Pf6N2E2_5480 0.56 +0.0 6,298,649 - Pf6N2E2_5480 0.56 +0.4 6,298,649 - Pf6N2E2_5480 0.56 -2.6 6,298,649 - Pf6N2E2_5480 0.56 -0.3 6,298,649 - Pf6N2E2_5480 0.56 -0.5 6,298,649 - Pf6N2E2_5480 0.56 -1.5 6,298,722 - Pf6N2E2_5480 0.63 +0.2 6,299,026 + +0.7 6,299,026 + +0.1 6,299,027 - +0.7 6,299,027 - +0.5
Or see this region's nucleotide sequence