Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5043 and Pf6N2E2_5044 are separated by 169 nucleotides Pf6N2E2_5044 and Pf6N2E2_5045 are separated by 161 nucleotides Pf6N2E2_5045 and Pf6N2E2_5046 are separated by 58 nucleotides
Pf6N2E2_5043 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 5,819,299 to 5,819,910
_5043
Pf6N2E2_5044 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 5,820,080 to 5,820,532
_5044
Pf6N2E2_5045 - Permease of the drug/metabolite transporter (DMT) superfamily, at 5,820,694 to 5,821,596
_5045
Pf6N2E2_5046 - ATP-dependent RNA helicase PA3950, at 5,821,655 to 5,822,992
_5046
Position (kb)
5820
5821
5822 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5819.908 kb on + strand at 5819.908 kb on + strand at 5819.909 kb on - strand at 5819.909 kb on - strand at 5819.909 kb on - strand at 5820.081 kb on + strand at 5820.081 kb on + strand at 5820.295 kb on - strand, within Pf6N2E2_5044 at 5820.384 kb on + strand, within Pf6N2E2_5044 at 5820.385 kb on - strand, within Pf6N2E2_5044 at 5820.457 kb on + strand, within Pf6N2E2_5044 at 5820.522 kb on + strand at 5820.523 kb on - strand at 5820.526 kb on + strand at 5820.526 kb on + strand at 5820.527 kb on - strand at 5820.527 kb on - strand at 5820.535 kb on - strand at 5820.535 kb on - strand at 5820.604 kb on + strand at 5820.615 kb on + strand at 5820.655 kb on + strand at 5820.655 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.748 kb on + strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.749 kb on - strand at 5820.769 kb on + strand at 5820.770 kb on - strand at 5820.853 kb on + strand, within Pf6N2E2_5045 at 5820.853 kb on + strand, within Pf6N2E2_5045 at 5820.854 kb on - strand, within Pf6N2E2_5045 at 5820.854 kb on - strand, within Pf6N2E2_5045 at 5821.027 kb on - strand, within Pf6N2E2_5045 at 5821.060 kb on - strand, within Pf6N2E2_5045 at 5821.088 kb on + strand, within Pf6N2E2_5045 at 5821.089 kb on - strand, within Pf6N2E2_5045 at 5821.089 kb on - strand, within Pf6N2E2_5045 at 5821.166 kb on + strand, within Pf6N2E2_5045 at 5821.348 kb on + strand, within Pf6N2E2_5045 at 5821.387 kb on + strand, within Pf6N2E2_5045 at 5821.387 kb on + strand, within Pf6N2E2_5045 at 5821.387 kb on + strand, within Pf6N2E2_5045 at 5821.387 kb on + strand, within Pf6N2E2_5045 at 5821.387 kb on + strand, within Pf6N2E2_5045 at 5821.388 kb on - strand, within Pf6N2E2_5045 at 5821.388 kb on - strand, within Pf6N2E2_5045 at 5821.388 kb on - strand, within Pf6N2E2_5045 at 5821.388 kb on - strand, within Pf6N2E2_5045 at 5821.388 kb on - strand, within Pf6N2E2_5045 at 5821.388 kb on - strand, within Pf6N2E2_5045 at 5821.388 kb on - strand, within Pf6N2E2_5045 at 5821.388 kb on - strand, within Pf6N2E2_5045 at 5821.393 kb on + strand, within Pf6N2E2_5045 at 5821.393 kb on + strand, within Pf6N2E2_5045 at 5821.393 kb on + strand, within Pf6N2E2_5045 at 5821.393 kb on + strand, within Pf6N2E2_5045 at 5821.394 kb on - strand, within Pf6N2E2_5045 at 5821.394 kb on - strand, within Pf6N2E2_5045 at 5821.394 kb on - strand, within Pf6N2E2_5045 at 5821.394 kb on - strand, within Pf6N2E2_5045 at 5821.394 kb on - strand, within Pf6N2E2_5045 at 5821.394 kb on - strand, within Pf6N2E2_5045 at 5821.394 kb on - strand, within Pf6N2E2_5045 at 5821.394 kb on - strand, within Pf6N2E2_5045 at 5821.432 kb on + strand, within Pf6N2E2_5045 at 5821.432 kb on + strand, within Pf6N2E2_5045 at 5821.432 kb on + strand, within Pf6N2E2_5045 at 5821.432 kb on + strand, within Pf6N2E2_5045 at 5821.433 kb on - strand, within Pf6N2E2_5045 at 5821.433 kb on - strand, within Pf6N2E2_5045 at 5821.433 kb on - strand, within Pf6N2E2_5045 at 5821.433 kb on - strand, within Pf6N2E2_5045 at 5821.433 kb on - strand, within Pf6N2E2_5045 at 5821.433 kb on - strand, within Pf6N2E2_5045 at 5821.433 kb on - strand, within Pf6N2E2_5045 at 5821.433 kb on - strand, within Pf6N2E2_5045 at 5821.438 kb on + strand, within Pf6N2E2_5045 at 5821.438 kb on + strand, within Pf6N2E2_5045 at 5821.438 kb on + strand, within Pf6N2E2_5045 at 5821.439 kb on - strand, within Pf6N2E2_5045 at 5821.439 kb on - strand, within Pf6N2E2_5045 at 5821.439 kb on - strand, within Pf6N2E2_5045 at 5821.600 kb on - strand at 5821.604 kb on + strand at 5821.605 kb on - strand at 5821.643 kb on + strand at 5821.648 kb on + strand at 5821.648 kb on + strand at 5821.648 kb on + strand at 5821.648 kb on + strand at 5821.649 kb on - strand at 5821.943 kb on + strand, within Pf6N2E2_5046 at 5821.970 kb on + strand, within Pf6N2E2_5046 at 5821.970 kb on + strand, within Pf6N2E2_5046 at 5821.970 kb on + strand, within Pf6N2E2_5046 at 5821.971 kb on - strand, within Pf6N2E2_5046 at 5821.971 kb on - strand, within Pf6N2E2_5046 at 5821.971 kb on - strand, within Pf6N2E2_5046 at 5821.971 kb on - strand, within Pf6N2E2_5046 at 5822.167 kb on + strand, within Pf6N2E2_5046 at 5822.167 kb on + strand, within Pf6N2E2_5046 at 5822.167 kb on + strand, within Pf6N2E2_5046 at 5822.168 kb on - strand, within Pf6N2E2_5046 at 5822.171 kb on + strand, within Pf6N2E2_5046 at 5822.171 kb on + strand, within Pf6N2E2_5046 at 5822.171 kb on + strand, within Pf6N2E2_5046 at 5822.172 kb on - strand, within Pf6N2E2_5046 at 5822.172 kb on - strand, within Pf6N2E2_5046 at 5822.172 kb on - strand, within Pf6N2E2_5046
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 5,819,908 + -1.3 5,819,908 + +0.4 5,819,909 - +1.2 5,819,909 - -1.5 5,819,909 - +0.1 5,820,081 + -0.6 5,820,081 + -0.8 5,820,295 - Pf6N2E2_5044 0.47 -0.3 5,820,384 + Pf6N2E2_5044 0.67 -0.8 5,820,385 - Pf6N2E2_5044 0.67 -1.0 5,820,457 + Pf6N2E2_5044 0.83 -0.8 5,820,522 + +2.1 5,820,523 - +0.1 5,820,526 + +1.1 5,820,526 + +1.0 5,820,527 - +0.2 5,820,527 - -0.1 5,820,535 - +0.6 5,820,535 - +0.3 5,820,604 + +0.5 5,820,615 + -0.1 5,820,655 + +0.1 5,820,655 + +1.9 5,820,748 + -0.7 5,820,748 + +1.6 5,820,748 + +0.8 5,820,748 + -0.1 5,820,748 + +0.6 5,820,748 + -0.5 5,820,748 + -0.7 5,820,748 + +0.7 5,820,748 + +0.9 5,820,748 + -0.1 5,820,748 + +0.8 5,820,748 + +1.7 5,820,749 - -0.5 5,820,749 - -1.2 5,820,749 - +0.1 5,820,749 - +0.1 5,820,749 - +0.9 5,820,749 - -0.2 5,820,749 - +0.5 5,820,749 - +0.8 5,820,769 + +0.9 5,820,770 - +1.3 5,820,853 + Pf6N2E2_5045 0.18 -0.5 5,820,853 + Pf6N2E2_5045 0.18 +0.1 5,820,854 - Pf6N2E2_5045 0.18 -0.4 5,820,854 - Pf6N2E2_5045 0.18 +0.3 5,821,027 - Pf6N2E2_5045 0.37 -0.7 5,821,060 - Pf6N2E2_5045 0.41 -0.1 5,821,088 + Pf6N2E2_5045 0.44 +0.7 5,821,089 - Pf6N2E2_5045 0.44 -0.7 5,821,089 - Pf6N2E2_5045 0.44 -0.2 5,821,166 + Pf6N2E2_5045 0.52 -0.2 5,821,348 + Pf6N2E2_5045 0.72 +0.6 5,821,387 + Pf6N2E2_5045 0.77 +0.3 5,821,387 + Pf6N2E2_5045 0.77 +0.2 5,821,387 + Pf6N2E2_5045 0.77 -0.4 5,821,387 + Pf6N2E2_5045 0.77 -0.8 5,821,387 + Pf6N2E2_5045 0.77 +0.4 5,821,388 - Pf6N2E2_5045 0.77 +0.9 5,821,388 - Pf6N2E2_5045 0.77 -2.3 5,821,388 - Pf6N2E2_5045 0.77 -1.4 5,821,388 - Pf6N2E2_5045 0.77 -0.3 5,821,388 - Pf6N2E2_5045 0.77 +0.3 5,821,388 - Pf6N2E2_5045 0.77 +0.5 5,821,388 - Pf6N2E2_5045 0.77 +0.5 5,821,388 - Pf6N2E2_5045 0.77 -0.2 5,821,393 + Pf6N2E2_5045 0.77 -0.8 5,821,393 + Pf6N2E2_5045 0.77 +0.1 5,821,393 + Pf6N2E2_5045 0.77 -0.1 5,821,393 + Pf6N2E2_5045 0.77 -0.1 5,821,394 - Pf6N2E2_5045 0.78 +1.0 5,821,394 - Pf6N2E2_5045 0.78 -1.2 5,821,394 - Pf6N2E2_5045 0.78 +0.7 5,821,394 - Pf6N2E2_5045 0.78 -0.7 5,821,394 - Pf6N2E2_5045 0.78 -0.2 5,821,394 - Pf6N2E2_5045 0.78 +0.2 5,821,394 - Pf6N2E2_5045 0.78 +0.3 5,821,394 - Pf6N2E2_5045 0.78 +1.2 5,821,432 + Pf6N2E2_5045 0.82 -0.6 5,821,432 + Pf6N2E2_5045 0.82 +1.1 5,821,432 + Pf6N2E2_5045 0.82 -0.6 5,821,432 + Pf6N2E2_5045 0.82 -1.6 5,821,433 - Pf6N2E2_5045 0.82 -0.3 5,821,433 - Pf6N2E2_5045 0.82 +0.3 5,821,433 - Pf6N2E2_5045 0.82 +0.3 5,821,433 - Pf6N2E2_5045 0.82 +0.5 5,821,433 - Pf6N2E2_5045 0.82 -0.5 5,821,433 - Pf6N2E2_5045 0.82 +0.7 5,821,433 - Pf6N2E2_5045 0.82 +0.1 5,821,433 - Pf6N2E2_5045 0.82 +0.6 5,821,438 + Pf6N2E2_5045 0.82 -1.3 5,821,438 + Pf6N2E2_5045 0.82 -0.5 5,821,438 + Pf6N2E2_5045 0.82 -1.3 5,821,439 - Pf6N2E2_5045 0.83 -0.3 5,821,439 - Pf6N2E2_5045 0.83 -0.2 5,821,439 - Pf6N2E2_5045 0.83 +1.1 5,821,600 - +1.2 5,821,604 + -0.4 5,821,605 - +1.1 5,821,643 + -0.6 5,821,648 + -1.1 5,821,648 + -0.5 5,821,648 + -0.1 5,821,648 + -0.2 5,821,649 - +0.2 5,821,943 + Pf6N2E2_5046 0.22 +0.2 5,821,970 + Pf6N2E2_5046 0.24 -1.6 5,821,970 + Pf6N2E2_5046 0.24 +0.1 5,821,970 + Pf6N2E2_5046 0.24 +1.2 5,821,971 - Pf6N2E2_5046 0.24 +1.4 5,821,971 - Pf6N2E2_5046 0.24 +1.2 5,821,971 - Pf6N2E2_5046 0.24 +0.9 5,821,971 - Pf6N2E2_5046 0.24 +0.7 5,822,167 + Pf6N2E2_5046 0.38 -0.0 5,822,167 + Pf6N2E2_5046 0.38 -0.1 5,822,167 + Pf6N2E2_5046 0.38 -0.3 5,822,168 - Pf6N2E2_5046 0.38 +0.3 5,822,171 + Pf6N2E2_5046 0.39 +1.2 5,822,171 + Pf6N2E2_5046 0.39 +1.8 5,822,171 + Pf6N2E2_5046 0.39 +1.5 5,822,172 - Pf6N2E2_5046 0.39 +0.1 5,822,172 - Pf6N2E2_5046 0.39 +0.4 5,822,172 - Pf6N2E2_5046 0.39 +0.6
Or see this region's nucleotide sequence