Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4999 and Pf6N2E2_5000 overlap by 1 nucleotides Pf6N2E2_5000 and Pf6N2E2_5001 are separated by 168 nucleotides
Pf6N2E2_4999 - Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-), at 5,765,187 to 5,766,197
_4999
Pf6N2E2_5000 - Rare lipoprotein A precursor, at 5,766,197 to 5,767,222
_5000
Pf6N2E2_5001 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 5,767,391 to 5,768,548
_5001
Position (kb)
5766
5767
5768 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5765.217 kb on + strand at 5765.280 kb on + strand at 5765.280 kb on + strand at 5765.280 kb on + strand at 5765.280 kb on + strand at 5765.281 kb on - strand at 5765.332 kb on - strand, within Pf6N2E2_4999 at 5765.362 kb on + strand at 5765.363 kb on - strand, within Pf6N2E2_4999 at 5765.535 kb on + strand, within Pf6N2E2_4999 at 5765.594 kb on + strand, within Pf6N2E2_4999 at 5765.742 kb on + strand, within Pf6N2E2_4999 at 5765.742 kb on + strand, within Pf6N2E2_4999 at 5765.742 kb on + strand, within Pf6N2E2_4999 at 5765.743 kb on - strand, within Pf6N2E2_4999 at 5765.743 kb on - strand, within Pf6N2E2_4999 at 5765.793 kb on + strand, within Pf6N2E2_4999 at 5765.794 kb on - strand, within Pf6N2E2_4999 at 5765.828 kb on + strand, within Pf6N2E2_4999 at 5765.871 kb on + strand, within Pf6N2E2_4999 at 5765.872 kb on - strand, within Pf6N2E2_4999 at 5765.872 kb on - strand, within Pf6N2E2_4999 at 5766.081 kb on + strand, within Pf6N2E2_4999 at 5766.120 kb on + strand at 5766.120 kb on + strand at 5766.120 kb on + strand at 5766.122 kb on + strand at 5766.122 kb on + strand at 5766.138 kb on + strand at 5766.138 kb on + strand at 5766.138 kb on + strand at 5766.138 kb on + strand at 5766.151 kb on + strand at 5766.151 kb on + strand at 5766.151 kb on + strand at 5766.153 kb on + strand at 5766.153 kb on + strand at 5766.211 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.215 kb on + strand at 5766.219 kb on + strand at 5767.275 kb on + strand at 5767.275 kb on + strand at 5767.311 kb on + strand at 5767.311 kb on + strand at 5767.311 kb on + strand at 5767.312 kb on - strand at 5767.312 kb on - strand at 5767.602 kb on - strand, within Pf6N2E2_5001 at 5767.904 kb on + strand, within Pf6N2E2_5001
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 5,765,217 + -0.5 5,765,280 + -1.1 5,765,280 + +0.4 5,765,280 + +1.5 5,765,280 + -0.3 5,765,281 - -1.2 5,765,332 - Pf6N2E2_4999 0.14 -3.4 5,765,362 + +1.1 5,765,363 - Pf6N2E2_4999 0.17 -3.2 5,765,535 + Pf6N2E2_4999 0.34 +0.4 5,765,594 + Pf6N2E2_4999 0.40 -0.2 5,765,742 + Pf6N2E2_4999 0.55 +1.8 5,765,742 + Pf6N2E2_4999 0.55 +0.8 5,765,742 + Pf6N2E2_4999 0.55 -0.0 5,765,743 - Pf6N2E2_4999 0.55 -2.4 5,765,743 - Pf6N2E2_4999 0.55 +1.1 5,765,793 + Pf6N2E2_4999 0.60 +1.1 5,765,794 - Pf6N2E2_4999 0.60 -1.6 5,765,828 + Pf6N2E2_4999 0.63 +0.8 5,765,871 + Pf6N2E2_4999 0.68 -0.2 5,765,872 - Pf6N2E2_4999 0.68 -2.5 5,765,872 - Pf6N2E2_4999 0.68 -3.7 5,766,081 + Pf6N2E2_4999 0.88 -0.6 5,766,120 + -0.0 5,766,120 + -0.8 5,766,120 + -0.9 5,766,122 + -1.3 5,766,122 + -0.9 5,766,138 + -0.4 5,766,138 + +0.1 5,766,138 + -1.2 5,766,138 + +0.2 5,766,151 + -1.6 5,766,151 + -0.7 5,766,151 + +0.1 5,766,153 + -1.8 5,766,153 + -1.1 5,766,211 + -2.0 5,766,215 + -2.1 5,766,215 + -1.9 5,766,215 + -1.9 5,766,215 + -2.0 5,766,215 + -2.4 5,766,215 + -4.8 5,766,215 + -1.7 5,766,215 + -0.2 5,766,215 + -2.3 5,766,215 + -2.3 5,766,215 + -2.6 5,766,219 + -3.1 5,767,275 + -1.2 5,767,275 + +1.7 5,767,311 + +0.7 5,767,311 + -1.0 5,767,311 + -0.6 5,767,312 - +3.2 5,767,312 - +3.7 5,767,602 - Pf6N2E2_5001 0.18 -1.5 5,767,904 + Pf6N2E2_5001 0.44 +0.6
Or see this region's nucleotide sequence