Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4997

Experiment: Vancomycin 0.24 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4995 and Pf6N2E2_4996 are separated by 9 nucleotidesPf6N2E2_4996 and Pf6N2E2_4997 are separated by 9 nucleotidesPf6N2E2_4997 and Pf6N2E2_4998 overlap by 11 nucleotides Pf6N2E2_4995 - Iojap protein, at 5,761,158 to 5,761,652 _4995 Pf6N2E2_4996 - LSU m3Psi1915 methyltransferase RlmH, at 5,761,662 to 5,762,129 _4996 Pf6N2E2_4997 - Penicillin-binding protein 2 (PBP-2), at 5,762,139 to 5,764,034 _4997 Pf6N2E2_4998 - Rod shape-determining protein RodA, at 5,764,024 to 5,765,169 _4998 Position (kb) 5762 5763 5764 5765Strain fitness (log2 ratio) -2 -1 0 1 2at 5761.728 kb on + strand, within Pf6N2E2_4996at 5761.728 kb on + strand, within Pf6N2E2_4996at 5761.728 kb on + strand, within Pf6N2E2_4996at 5761.728 kb on + strand, within Pf6N2E2_4996at 5761.728 kb on + strand, within Pf6N2E2_4996at 5762.085 kb on + strandat 5762.085 kb on + strandat 5762.127 kb on + strandat 5762.127 kb on + strandat 5762.127 kb on + strandat 5762.127 kb on + strandat 5762.127 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml
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5,761,728 + Pf6N2E2_4996 0.14 -0.2
5,761,728 + Pf6N2E2_4996 0.14 +0.5
5,761,728 + Pf6N2E2_4996 0.14 +1.5
5,761,728 + Pf6N2E2_4996 0.14 +0.7
5,761,728 + Pf6N2E2_4996 0.14 -0.1
5,762,085 + +2.7
5,762,085 + +1.1
5,762,127 + -1.7
5,762,127 + -1.7
5,762,127 + -2.2
5,762,127 + -1.1
5,762,127 + -0.2

Or see this region's nucleotide sequence