Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4841 and Pf6N2E2_4842 overlap by 11 nucleotides Pf6N2E2_4842 and Pf6N2E2_4843 are separated by 8 nucleotides Pf6N2E2_4843 and Pf6N2E2_6142 are separated by 142 nucleotides Pf6N2E2_6142 and Pf6N2E2_6143 are separated by 27 nucleotides Pf6N2E2_6143 and Pf6N2E2_6144 are separated by 29 nucleotides Pf6N2E2_6144 and Pf6N2E2_4844 are separated by 87 nucleotides
Pf6N2E2_4841 - Biotin operon repressor / Biotin-protein ligase (EC 6.3.4.15), at 5,599,750 to 5,600,793
_4841
Pf6N2E2_4842 - Pantothenate kinase type III, CoaX-like (EC 2.7.1.33), at 5,600,783 to 5,601,532
_4842
Pf6N2E2_4843 - FIG00956941: hypothetical protein, at 5,601,541 to 5,601,996
_4843
Pf6N2E2_6142 - tRNA-Tyr-GTA, at 5,602,139 to 5,602,220
_6142
Pf6N2E2_6143 - tRNA-Gly-TCC, at 5,602,248 to 5,602,318
_6143
Pf6N2E2_6144 - tRNA-Thr-GGT, at 5,602,348 to 5,602,420
_6144
Pf6N2E2_4844 - Translation elongation factor Tu, at 5,602,508 to 5,603,701
_4844
Position (kb)
5601
5602 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5601.540 kb on + strand at 5601.540 kb on + strand at 5601.583 kb on + strand at 5601.583 kb on + strand at 5601.583 kb on + strand at 5601.583 kb on + strand at 5601.583 kb on + strand at 5601.583 kb on + strand at 5601.584 kb on - strand at 5601.584 kb on - strand at 5601.586 kb on + strand at 5601.586 kb on + strand at 5601.586 kb on + strand at 5601.586 kb on + strand at 5601.586 kb on + strand at 5601.586 kb on + strand at 5601.587 kb on - strand, within Pf6N2E2_4843 at 5601.587 kb on - strand, within Pf6N2E2_4843 at 5601.646 kb on + strand, within Pf6N2E2_4843 at 5601.646 kb on + strand, within Pf6N2E2_4843 at 5601.718 kb on - strand, within Pf6N2E2_4843 at 5601.748 kb on + strand, within Pf6N2E2_4843 at 5601.748 kb on + strand, within Pf6N2E2_4843 at 5601.749 kb on - strand, within Pf6N2E2_4843 at 5601.818 kb on + strand, within Pf6N2E2_4843 at 5601.818 kb on + strand, within Pf6N2E2_4843 at 5601.933 kb on + strand, within Pf6N2E2_4843 at 5602.003 kb on + strand at 5602.426 kb on + strand at 5602.426 kb on + strand at 5602.426 kb on + strand at 5602.438 kb on + strand at 5602.438 kb on + strand at 5602.446 kb on + strand at 5602.446 kb on + strand at 5602.446 kb on + strand at 5602.446 kb on + strand at 5602.461 kb on + strand at 5602.461 kb on + strand at 5602.461 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 5,601,540 + -1.7 5,601,540 + -4.2 5,601,583 + -2.2 5,601,583 + -2.5 5,601,583 + -5.1 5,601,583 + -2.7 5,601,583 + -2.3 5,601,583 + -2.7 5,601,584 - -0.9 5,601,584 - -2.7 5,601,586 + -1.8 5,601,586 + -1.0 5,601,586 + -1.4 5,601,586 + +0.7 5,601,586 + -0.8 5,601,586 + -1.2 5,601,587 - Pf6N2E2_4843 0.10 -0.7 5,601,587 - Pf6N2E2_4843 0.10 +0.1 5,601,646 + Pf6N2E2_4843 0.23 -2.6 5,601,646 + Pf6N2E2_4843 0.23 -1.3 5,601,718 - Pf6N2E2_4843 0.39 -2.3 5,601,748 + Pf6N2E2_4843 0.45 -0.8 5,601,748 + Pf6N2E2_4843 0.45 -2.0 5,601,749 - Pf6N2E2_4843 0.46 -0.9 5,601,818 + Pf6N2E2_4843 0.61 -0.8 5,601,818 + Pf6N2E2_4843 0.61 -2.9 5,601,933 + Pf6N2E2_4843 0.86 -1.5 5,602,003 + +1.1 5,602,426 + +0.7 5,602,426 + +1.0 5,602,426 + +0.8 5,602,438 + +0.2 5,602,438 + -0.2 5,602,446 + -0.2 5,602,446 + +0.4 5,602,446 + +0.6 5,602,446 + +0.6 5,602,461 + +1.3 5,602,461 + +1.1 5,602,461 + +0.3
Or see this region's nucleotide sequence