Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4843

Experiment: Vancomycin 0.24 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4841 and Pf6N2E2_4842 overlap by 11 nucleotidesPf6N2E2_4842 and Pf6N2E2_4843 are separated by 8 nucleotidesPf6N2E2_4843 and Pf6N2E2_6142 are separated by 142 nucleotidesPf6N2E2_6142 and Pf6N2E2_6143 are separated by 27 nucleotidesPf6N2E2_6143 and Pf6N2E2_6144 are separated by 29 nucleotidesPf6N2E2_6144 and Pf6N2E2_4844 are separated by 87 nucleotides Pf6N2E2_4841 - Biotin operon repressor / Biotin-protein ligase (EC 6.3.4.15), at 5,599,750 to 5,600,793 _4841 Pf6N2E2_4842 - Pantothenate kinase type III, CoaX-like (EC 2.7.1.33), at 5,600,783 to 5,601,532 _4842 Pf6N2E2_4843 - FIG00956941: hypothetical protein, at 5,601,541 to 5,601,996 _4843 Pf6N2E2_6142 - tRNA-Tyr-GTA, at 5,602,139 to 5,602,220 _6142 Pf6N2E2_6143 - tRNA-Gly-TCC, at 5,602,248 to 5,602,318 _6143 Pf6N2E2_6144 - tRNA-Thr-GGT, at 5,602,348 to 5,602,420 _6144 Pf6N2E2_4844 - Translation elongation factor Tu, at 5,602,508 to 5,603,701 _4844 Position (kb) 5601 5602Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 5601.540 kb on + strandat 5601.540 kb on + strandat 5601.583 kb on + strandat 5601.583 kb on + strandat 5601.583 kb on + strandat 5601.583 kb on + strandat 5601.583 kb on + strandat 5601.583 kb on + strandat 5601.584 kb on - strandat 5601.584 kb on - strandat 5601.586 kb on + strandat 5601.586 kb on + strandat 5601.586 kb on + strandat 5601.586 kb on + strandat 5601.586 kb on + strandat 5601.586 kb on + strandat 5601.587 kb on - strand, within Pf6N2E2_4843at 5601.587 kb on - strand, within Pf6N2E2_4843at 5601.646 kb on + strand, within Pf6N2E2_4843at 5601.646 kb on + strand, within Pf6N2E2_4843at 5601.718 kb on - strand, within Pf6N2E2_4843at 5601.748 kb on + strand, within Pf6N2E2_4843at 5601.748 kb on + strand, within Pf6N2E2_4843at 5601.749 kb on - strand, within Pf6N2E2_4843at 5601.818 kb on + strand, within Pf6N2E2_4843at 5601.818 kb on + strand, within Pf6N2E2_4843at 5601.933 kb on + strand, within Pf6N2E2_4843at 5602.003 kb on + strandat 5602.426 kb on + strandat 5602.426 kb on + strandat 5602.426 kb on + strandat 5602.438 kb on + strandat 5602.438 kb on + strandat 5602.446 kb on + strandat 5602.446 kb on + strandat 5602.446 kb on + strandat 5602.446 kb on + strandat 5602.461 kb on + strandat 5602.461 kb on + strandat 5602.461 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml
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5,601,540 + -1.7
5,601,540 + -4.2
5,601,583 + -2.2
5,601,583 + -2.5
5,601,583 + -5.1
5,601,583 + -2.7
5,601,583 + -2.3
5,601,583 + -2.7
5,601,584 - -0.9
5,601,584 - -2.7
5,601,586 + -1.8
5,601,586 + -1.0
5,601,586 + -1.4
5,601,586 + +0.7
5,601,586 + -0.8
5,601,586 + -1.2
5,601,587 - Pf6N2E2_4843 0.10 -0.7
5,601,587 - Pf6N2E2_4843 0.10 +0.1
5,601,646 + Pf6N2E2_4843 0.23 -2.6
5,601,646 + Pf6N2E2_4843 0.23 -1.3
5,601,718 - Pf6N2E2_4843 0.39 -2.3
5,601,748 + Pf6N2E2_4843 0.45 -0.8
5,601,748 + Pf6N2E2_4843 0.45 -2.0
5,601,749 - Pf6N2E2_4843 0.46 -0.9
5,601,818 + Pf6N2E2_4843 0.61 -0.8
5,601,818 + Pf6N2E2_4843 0.61 -2.9
5,601,933 + Pf6N2E2_4843 0.86 -1.5
5,602,003 + +1.1
5,602,426 + +0.7
5,602,426 + +1.0
5,602,426 + +0.8
5,602,438 + +0.2
5,602,438 + -0.2
5,602,446 + -0.2
5,602,446 + +0.4
5,602,446 + +0.6
5,602,446 + +0.6
5,602,461 + +1.3
5,602,461 + +1.1
5,602,461 + +0.3

Or see this region's nucleotide sequence