Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3819 and Pf6N2E2_3820 are separated by 166 nucleotides Pf6N2E2_3820 and Pf6N2E2_3821 overlap by 33 nucleotides Pf6N2E2_3821 and Pf6N2E2_3822 are separated by 121 nucleotides Pf6N2E2_3822 and Pf6N2E2_3823 are separated by 103 nucleotides
Pf6N2E2_3819 - Thiamine biosynthesis protein thiI, at 4,454,493 to 4,455,947
_3819
Pf6N2E2_3820 - hypothetical protein, at 4,456,114 to 4,456,308
_3820
Pf6N2E2_3821 - Glutamine synthetase type I (EC 6.3.1.2), at 4,456,276 to 4,457,682
_3821
Pf6N2E2_3822 - Periplasmic chorismate mutase I precursor (EC 5.4.99.5), at 4,457,804 to 4,458,367
_3822
Pf6N2E2_3823 - FIG00956533: hypothetical protein, at 4,458,471 to 4,458,989
_3823
Position (kb)
4456
4457
4458 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4455.993 kb on - strand at 4456.094 kb on - strand at 4456.115 kb on + strand at 4456.115 kb on + strand at 4456.116 kb on - strand at 4456.209 kb on + strand, within Pf6N2E2_3820 at 4456.230 kb on + strand, within Pf6N2E2_3820 at 4456.234 kb on + strand, within Pf6N2E2_3820 at 4456.234 kb on + strand, within Pf6N2E2_3820 at 4456.275 kb on + strand, within Pf6N2E2_3820 at 4457.688 kb on + strand at 4457.689 kb on - strand at 4457.758 kb on - strand at 4457.786 kb on + strand at 4457.786 kb on + strand at 4457.786 kb on + strand at 4457.786 kb on + strand at 4457.786 kb on + strand at 4457.787 kb on - strand at 4457.787 kb on - strand at 4457.787 kb on - strand at 4457.787 kb on - strand at 4457.788 kb on + strand at 4457.788 kb on + strand at 4457.788 kb on + strand at 4457.788 kb on + strand at 4457.788 kb on + strand at 4457.788 kb on + strand at 4457.788 kb on + strand at 4457.788 kb on + strand at 4457.788 kb on + strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.789 kb on - strand at 4457.971 kb on + strand, within Pf6N2E2_3822 at 4457.971 kb on + strand, within Pf6N2E2_3822 at 4457.971 kb on + strand, within Pf6N2E2_3822 at 4457.971 kb on + strand, within Pf6N2E2_3822 at 4457.972 kb on - strand, within Pf6N2E2_3822 at 4458.038 kb on + strand, within Pf6N2E2_3822 at 4458.039 kb on - strand, within Pf6N2E2_3822 at 4458.039 kb on - strand, within Pf6N2E2_3822 at 4458.084 kb on + strand, within Pf6N2E2_3822 at 4458.084 kb on + strand, within Pf6N2E2_3822 at 4458.084 kb on + strand, within Pf6N2E2_3822 at 4458.085 kb on - strand, within Pf6N2E2_3822 at 4458.085 kb on - strand, within Pf6N2E2_3822 at 4458.085 kb on - strand, within Pf6N2E2_3822 at 4458.107 kb on + strand, within Pf6N2E2_3822 at 4458.107 kb on + strand, within Pf6N2E2_3822 at 4458.107 kb on + strand, within Pf6N2E2_3822 at 4458.107 kb on + strand, within Pf6N2E2_3822 at 4458.107 kb on + strand, within Pf6N2E2_3822 at 4458.108 kb on - strand, within Pf6N2E2_3822 at 4458.108 kb on - strand, within Pf6N2E2_3822 at 4458.108 kb on - strand, within Pf6N2E2_3822 at 4458.108 kb on - strand, within Pf6N2E2_3822 at 4458.108 kb on - strand, within Pf6N2E2_3822 at 4458.108 kb on - strand, within Pf6N2E2_3822 at 4458.108 kb on - strand, within Pf6N2E2_3822 at 4458.395 kb on + strand at 4458.395 kb on + strand at 4458.395 kb on + strand at 4458.396 kb on - strand at 4458.396 kb on - strand at 4458.432 kb on + strand at 4458.432 kb on + strand at 4458.432 kb on + strand at 4458.432 kb on + strand at 4458.432 kb on + strand at 4458.432 kb on + strand at 4458.433 kb on - strand at 4458.433 kb on - strand at 4458.433 kb on - strand at 4458.433 kb on - strand at 4458.433 kb on - strand at 4458.534 kb on + strand, within Pf6N2E2_3823 at 4458.534 kb on + strand, within Pf6N2E2_3823 at 4458.534 kb on + strand, within Pf6N2E2_3823 at 4458.534 kb on + strand, within Pf6N2E2_3823 at 4458.534 kb on + strand, within Pf6N2E2_3823 at 4458.534 kb on + strand, within Pf6N2E2_3823 at 4458.535 kb on - strand, within Pf6N2E2_3823 at 4458.535 kb on - strand, within Pf6N2E2_3823 at 4458.540 kb on + strand, within Pf6N2E2_3823 at 4458.540 kb on + strand, within Pf6N2E2_3823 at 4458.541 kb on - strand, within Pf6N2E2_3823 at 4458.541 kb on - strand, within Pf6N2E2_3823 at 4458.541 kb on - strand, within Pf6N2E2_3823 at 4458.541 kb on - strand, within Pf6N2E2_3823 at 4458.567 kb on + strand, within Pf6N2E2_3823 at 4458.567 kb on + strand, within Pf6N2E2_3823 at 4458.567 kb on + strand, within Pf6N2E2_3823 at 4458.567 kb on + strand, within Pf6N2E2_3823 at 4458.568 kb on - strand, within Pf6N2E2_3823
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 4,455,993 - +0.3 4,456,094 - +1.1 4,456,115 + +0.4 4,456,115 + -2.8 4,456,116 - -0.7 4,456,209 + Pf6N2E2_3820 0.49 -0.5 4,456,230 + Pf6N2E2_3820 0.59 +1.0 4,456,234 + Pf6N2E2_3820 0.62 -0.6 4,456,234 + Pf6N2E2_3820 0.62 -0.5 4,456,275 + Pf6N2E2_3820 0.83 -1.1 4,457,688 + +2.4 4,457,689 - +1.1 4,457,758 - -0.8 4,457,786 + +0.2 4,457,786 + -0.9 4,457,786 + -0.9 4,457,786 + +0.2 4,457,786 + +0.2 4,457,787 - +1.0 4,457,787 - -0.4 4,457,787 - +0.9 4,457,787 - +1.1 4,457,788 + -0.8 4,457,788 + +0.4 4,457,788 + +0.8 4,457,788 + -0.9 4,457,788 + -0.5 4,457,788 + -1.9 4,457,788 + -0.7 4,457,788 + +0.7 4,457,788 + -0.9 4,457,789 - -0.4 4,457,789 - -2.9 4,457,789 - -0.7 4,457,789 - -0.3 4,457,789 - -0.1 4,457,789 - -0.3 4,457,789 - +0.6 4,457,789 - +0.2 4,457,789 - +0.5 4,457,789 - +1.2 4,457,789 - -0.2 4,457,789 - -0.8 4,457,971 + Pf6N2E2_3822 0.30 -1.1 4,457,971 + Pf6N2E2_3822 0.30 +0.4 4,457,971 + Pf6N2E2_3822 0.30 +0.2 4,457,971 + Pf6N2E2_3822 0.30 +0.3 4,457,972 - Pf6N2E2_3822 0.30 +0.2 4,458,038 + Pf6N2E2_3822 0.41 +0.8 4,458,039 - Pf6N2E2_3822 0.42 -0.7 4,458,039 - Pf6N2E2_3822 0.42 +1.4 4,458,084 + Pf6N2E2_3822 0.50 -0.4 4,458,084 + Pf6N2E2_3822 0.50 +1.7 4,458,084 + Pf6N2E2_3822 0.50 -1.4 4,458,085 - Pf6N2E2_3822 0.50 +1.1 4,458,085 - Pf6N2E2_3822 0.50 +1.4 4,458,085 - Pf6N2E2_3822 0.50 -0.7 4,458,107 + Pf6N2E2_3822 0.54 +0.3 4,458,107 + Pf6N2E2_3822 0.54 +0.5 4,458,107 + Pf6N2E2_3822 0.54 -1.2 4,458,107 + Pf6N2E2_3822 0.54 +0.5 4,458,107 + Pf6N2E2_3822 0.54 -0.0 4,458,108 - Pf6N2E2_3822 0.54 -0.7 4,458,108 - Pf6N2E2_3822 0.54 -0.9 4,458,108 - Pf6N2E2_3822 0.54 +0.2 4,458,108 - Pf6N2E2_3822 0.54 +0.2 4,458,108 - Pf6N2E2_3822 0.54 +1.2 4,458,108 - Pf6N2E2_3822 0.54 -0.1 4,458,108 - Pf6N2E2_3822 0.54 -1.1 4,458,395 + -1.3 4,458,395 + +0.8 4,458,395 + +0.5 4,458,396 - +0.2 4,458,396 - +0.7 4,458,432 + -0.8 4,458,432 + -0.2 4,458,432 + +1.2 4,458,432 + -0.9 4,458,432 + +1.0 4,458,432 + +1.9 4,458,433 - -1.7 4,458,433 - -0.4 4,458,433 - -0.1 4,458,433 - +0.1 4,458,433 - +0.4 4,458,534 + Pf6N2E2_3823 0.12 -1.3 4,458,534 + Pf6N2E2_3823 0.12 -1.5 4,458,534 + Pf6N2E2_3823 0.12 +0.3 4,458,534 + Pf6N2E2_3823 0.12 +0.6 4,458,534 + Pf6N2E2_3823 0.12 +1.3 4,458,534 + Pf6N2E2_3823 0.12 -0.8 4,458,535 - Pf6N2E2_3823 0.12 +1.5 4,458,535 - Pf6N2E2_3823 0.12 +0.9 4,458,540 + Pf6N2E2_3823 0.13 -0.2 4,458,540 + Pf6N2E2_3823 0.13 -0.6 4,458,541 - Pf6N2E2_3823 0.13 +0.2 4,458,541 - Pf6N2E2_3823 0.13 +0.7 4,458,541 - Pf6N2E2_3823 0.13 -0.8 4,458,541 - Pf6N2E2_3823 0.13 +1.4 4,458,567 + Pf6N2E2_3823 0.18 -0.4 4,458,567 + Pf6N2E2_3823 0.18 +2.0 4,458,567 + Pf6N2E2_3823 0.18 +0.6 4,458,567 + Pf6N2E2_3823 0.18 +0.4 4,458,568 - Pf6N2E2_3823 0.19 -0.3
Or see this region's nucleotide sequence