Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3797 and Pf6N2E2_3798 are separated by 11 nucleotides Pf6N2E2_3798 and Pf6N2E2_3799 are separated by 136 nucleotides
Pf6N2E2_3797 - N-formylglutamate deformylase (EC 3.5.1.68) (from data) , at 4,423,449 to 4,424,252
_3797
Pf6N2E2_3798 - Imidazolonepropionase (EC 3.5.2.7) (from data) , at 4,424,264 to 4,425,466
_3798
Pf6N2E2_3799 - Histidine ammonia-lyase (EC 4.3.1.3) (from data) , at 4,425,603 to 4,427,135
_3799
Position (kb)
4424
4425
4426 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4423.459 kb on + strand at 4423.459 kb on + strand at 4423.459 kb on + strand at 4423.459 kb on + strand at 4423.460 kb on - strand at 4423.460 kb on - strand at 4423.460 kb on - strand at 4423.460 kb on - strand at 4423.460 kb on - strand at 4423.460 kb on - strand at 4423.460 kb on - strand at 4423.460 kb on - strand at 4423.540 kb on + strand, within Pf6N2E2_3797 at 4423.540 kb on + strand, within Pf6N2E2_3797 at 4423.555 kb on + strand, within Pf6N2E2_3797 at 4423.555 kb on + strand, within Pf6N2E2_3797 at 4423.555 kb on + strand, within Pf6N2E2_3797 at 4423.555 kb on + strand, within Pf6N2E2_3797 at 4423.556 kb on - strand, within Pf6N2E2_3797 at 4423.556 kb on - strand, within Pf6N2E2_3797 at 4423.556 kb on - strand, within Pf6N2E2_3797 at 4423.567 kb on + strand, within Pf6N2E2_3797 at 4423.567 kb on + strand, within Pf6N2E2_3797 at 4423.567 kb on + strand, within Pf6N2E2_3797 at 4423.567 kb on + strand, within Pf6N2E2_3797 at 4423.567 kb on + strand, within Pf6N2E2_3797 at 4423.568 kb on - strand, within Pf6N2E2_3797 at 4423.568 kb on - strand, within Pf6N2E2_3797 at 4423.624 kb on + strand, within Pf6N2E2_3797 at 4423.635 kb on - strand, within Pf6N2E2_3797 at 4423.832 kb on - strand, within Pf6N2E2_3797 at 4423.883 kb on - strand, within Pf6N2E2_3797 at 4423.906 kb on + strand, within Pf6N2E2_3797 at 4423.906 kb on + strand, within Pf6N2E2_3797 at 4423.907 kb on - strand, within Pf6N2E2_3797 at 4423.907 kb on - strand, within Pf6N2E2_3797 at 4424.005 kb on + strand, within Pf6N2E2_3797 at 4424.005 kb on + strand, within Pf6N2E2_3797 at 4424.005 kb on + strand, within Pf6N2E2_3797 at 4424.005 kb on + strand, within Pf6N2E2_3797 at 4424.101 kb on + strand, within Pf6N2E2_3797 at 4424.102 kb on - strand, within Pf6N2E2_3797 at 4424.102 kb on - strand, within Pf6N2E2_3797 at 4424.322 kb on + strand at 4424.323 kb on - strand at 4424.476 kb on + strand, within Pf6N2E2_3798 at 4424.476 kb on + strand, within Pf6N2E2_3798 at 4424.476 kb on + strand, within Pf6N2E2_3798 at 4424.477 kb on - strand, within Pf6N2E2_3798 at 4424.477 kb on - strand, within Pf6N2E2_3798 at 4424.477 kb on - strand, within Pf6N2E2_3798 at 4424.477 kb on - strand, within Pf6N2E2_3798 at 4424.477 kb on - strand, within Pf6N2E2_3798 at 4424.611 kb on - strand, within Pf6N2E2_3798 at 4424.835 kb on + strand, within Pf6N2E2_3798 at 4424.916 kb on + strand, within Pf6N2E2_3798 at 4424.916 kb on + strand, within Pf6N2E2_3798 at 4424.916 kb on + strand, within Pf6N2E2_3798 at 4424.916 kb on + strand, within Pf6N2E2_3798 at 4424.917 kb on - strand, within Pf6N2E2_3798 at 4424.917 kb on - strand, within Pf6N2E2_3798 at 4424.938 kb on - strand, within Pf6N2E2_3798 at 4424.938 kb on - strand, within Pf6N2E2_3798 at 4424.979 kb on - strand, within Pf6N2E2_3798 at 4425.257 kb on + strand, within Pf6N2E2_3798 at 4425.257 kb on + strand, within Pf6N2E2_3798 at 4425.258 kb on - strand, within Pf6N2E2_3798 at 4425.258 kb on - strand, within Pf6N2E2_3798 at 4425.262 kb on + strand, within Pf6N2E2_3798 at 4425.263 kb on - strand, within Pf6N2E2_3798 at 4425.537 kb on + strand at 4425.604 kb on + strand at 4425.604 kb on + strand at 4425.605 kb on - strand at 4425.605 kb on - strand at 4425.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 4,423,459 + -0.4 4,423,459 + +1.6 4,423,459 + +1.0 4,423,459 + +0.5 4,423,460 - +1.4 4,423,460 - +0.1 4,423,460 - +0.9 4,423,460 - +1.0 4,423,460 - -1.5 4,423,460 - +0.5 4,423,460 - +0.4 4,423,460 - +1.2 4,423,540 + Pf6N2E2_3797 0.11 +1.3 4,423,540 + Pf6N2E2_3797 0.11 +0.5 4,423,555 + Pf6N2E2_3797 0.13 -0.9 4,423,555 + Pf6N2E2_3797 0.13 -1.1 4,423,555 + Pf6N2E2_3797 0.13 +0.5 4,423,555 + Pf6N2E2_3797 0.13 +1.0 4,423,556 - Pf6N2E2_3797 0.13 +0.4 4,423,556 - Pf6N2E2_3797 0.13 +1.2 4,423,556 - Pf6N2E2_3797 0.13 +2.1 4,423,567 + Pf6N2E2_3797 0.15 +1.2 4,423,567 + Pf6N2E2_3797 0.15 +0.4 4,423,567 + Pf6N2E2_3797 0.15 +1.2 4,423,567 + Pf6N2E2_3797 0.15 +0.2 4,423,567 + Pf6N2E2_3797 0.15 +1.5 4,423,568 - Pf6N2E2_3797 0.15 +0.8 4,423,568 - Pf6N2E2_3797 0.15 -0.9 4,423,624 + Pf6N2E2_3797 0.22 +0.5 4,423,635 - Pf6N2E2_3797 0.23 +0.1 4,423,832 - Pf6N2E2_3797 0.48 -0.2 4,423,883 - Pf6N2E2_3797 0.54 +0.6 4,423,906 + Pf6N2E2_3797 0.57 +1.3 4,423,906 + Pf6N2E2_3797 0.57 +0.0 4,423,907 - Pf6N2E2_3797 0.57 +0.0 4,423,907 - Pf6N2E2_3797 0.57 -0.8 4,424,005 + Pf6N2E2_3797 0.69 -0.7 4,424,005 + Pf6N2E2_3797 0.69 +1.5 4,424,005 + Pf6N2E2_3797 0.69 -0.4 4,424,005 + Pf6N2E2_3797 0.69 +1.3 4,424,101 + Pf6N2E2_3797 0.81 +1.7 4,424,102 - Pf6N2E2_3797 0.81 +0.2 4,424,102 - Pf6N2E2_3797 0.81 +1.4 4,424,322 + -2.7 4,424,323 - +0.6 4,424,476 + Pf6N2E2_3798 0.18 -0.8 4,424,476 + Pf6N2E2_3798 0.18 +0.1 4,424,476 + Pf6N2E2_3798 0.18 +0.9 4,424,477 - Pf6N2E2_3798 0.18 +0.5 4,424,477 - Pf6N2E2_3798 0.18 +0.6 4,424,477 - Pf6N2E2_3798 0.18 +0.9 4,424,477 - Pf6N2E2_3798 0.18 +1.2 4,424,477 - Pf6N2E2_3798 0.18 -2.1 4,424,611 - Pf6N2E2_3798 0.29 +0.9 4,424,835 + Pf6N2E2_3798 0.47 +0.9 4,424,916 + Pf6N2E2_3798 0.54 +0.6 4,424,916 + Pf6N2E2_3798 0.54 +1.0 4,424,916 + Pf6N2E2_3798 0.54 +0.4 4,424,916 + Pf6N2E2_3798 0.54 -0.4 4,424,917 - Pf6N2E2_3798 0.54 +1.0 4,424,917 - Pf6N2E2_3798 0.54 +1.3 4,424,938 - Pf6N2E2_3798 0.56 +0.7 4,424,938 - Pf6N2E2_3798 0.56 -0.1 4,424,979 - Pf6N2E2_3798 0.59 +0.5 4,425,257 + Pf6N2E2_3798 0.83 -0.4 4,425,257 + Pf6N2E2_3798 0.83 +1.6 4,425,258 - Pf6N2E2_3798 0.83 +0.1 4,425,258 - Pf6N2E2_3798 0.83 +0.7 4,425,262 + Pf6N2E2_3798 0.83 +1.6 4,425,263 - Pf6N2E2_3798 0.83 +0.4 4,425,537 + +1.4 4,425,604 + -1.3 4,425,604 + +0.7 4,425,605 - -1.2 4,425,605 - -0.4 4,425,709 + +0.4
Or see this region's nucleotide sequence