Experiment: Vancomycin 0.24 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3585 and Pf6N2E2_3586 are separated by 4 nucleotides Pf6N2E2_3586 and Pf6N2E2_3587 are separated by 319 nucleotides Pf6N2E2_3587 and Pf6N2E2_3588 are separated by 143 nucleotides
Pf6N2E2_3585 - Nicotinate phosphoribosyltransferase (EC 2.4.2.11), at 4,176,638 to 4,177,864
_3585
Pf6N2E2_3586 - NAD synthetase (EC 6.3.1.5), at 4,177,869 to 4,178,696
_3586
Pf6N2E2_3587 - Azurin, at 4,179,016 to 4,179,462
_3587
Pf6N2E2_3588 - hypothetical protein, at 4,179,606 to 4,179,743
_3588
Position (kb)
4177
4178
4179 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4176.933 kb on - strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.964 kb on - strand, within Pf6N2E2_3585 at 4176.964 kb on - strand, within Pf6N2E2_3585 at 4177.392 kb on - strand, within Pf6N2E2_3585 at 4177.645 kb on + strand, within Pf6N2E2_3585 at 4177.646 kb on - strand, within Pf6N2E2_3585 at 4177.696 kb on - strand, within Pf6N2E2_3585 at 4177.696 kb on - strand, within Pf6N2E2_3585 at 4177.696 kb on - strand, within Pf6N2E2_3585 at 4177.717 kb on + strand, within Pf6N2E2_3585 at 4177.805 kb on + strand at 4177.805 kb on + strand at 4177.805 kb on + strand at 4177.805 kb on + strand at 4177.806 kb on - strand at 4177.827 kb on + strand at 4177.827 kb on + strand at 4177.828 kb on - strand at 4177.879 kb on + strand at 4177.970 kb on + strand, within Pf6N2E2_3586 at 4177.971 kb on - strand, within Pf6N2E2_3586 at 4178.106 kb on + strand, within Pf6N2E2_3586 at 4178.106 kb on + strand, within Pf6N2E2_3586 at 4178.297 kb on + strand, within Pf6N2E2_3586 at 4178.297 kb on + strand, within Pf6N2E2_3586 at 4178.315 kb on + strand, within Pf6N2E2_3586 at 4178.315 kb on + strand, within Pf6N2E2_3586 at 4178.315 kb on + strand, within Pf6N2E2_3586 at 4178.316 kb on - strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.329 kb on - strand, within Pf6N2E2_3586 at 4178.329 kb on - strand, within Pf6N2E2_3586 at 4178.329 kb on - strand, within Pf6N2E2_3586 at 4178.580 kb on + strand, within Pf6N2E2_3586 at 4178.580 kb on + strand, within Pf6N2E2_3586 at 4178.580 kb on + strand, within Pf6N2E2_3586 at 4178.581 kb on - strand, within Pf6N2E2_3586 at 4178.652 kb on + strand at 4178.652 kb on + strand at 4178.699 kb on - strand at 4178.799 kb on + strand at 4178.981 kb on - strand at 4179.080 kb on + strand, within Pf6N2E2_3587 at 4179.081 kb on - strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.189 kb on - strand, within Pf6N2E2_3587 at 4179.189 kb on - strand, within Pf6N2E2_3587 at 4179.440 kb on - strand at 4179.534 kb on + strand at 4179.564 kb on + strand at 4179.564 kb on + strand at 4179.571 kb on - strand at 4179.571 kb on - strand at 4179.618 kb on + strand at 4179.619 kb on - strand at 4179.619 kb on - strand at 4179.619 kb on - strand at 4179.619 kb on - strand at 4179.673 kb on + strand, within Pf6N2E2_3588 at 4179.673 kb on + strand, within Pf6N2E2_3588 at 4179.674 kb on - strand, within Pf6N2E2_3588
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.24 mg/ml remove 4,176,933 - Pf6N2E2_3585 0.24 +0.4 4,176,963 + Pf6N2E2_3585 0.26 -0.9 4,176,963 + Pf6N2E2_3585 0.26 +0.1 4,176,963 + Pf6N2E2_3585 0.26 +0.1 4,176,963 + Pf6N2E2_3585 0.26 -0.8 4,176,963 + Pf6N2E2_3585 0.26 -0.1 4,176,963 + Pf6N2E2_3585 0.26 +0.3 4,176,963 + Pf6N2E2_3585 0.26 +0.0 4,176,964 - Pf6N2E2_3585 0.27 -0.5 4,176,964 - Pf6N2E2_3585 0.27 +0.2 4,177,392 - Pf6N2E2_3585 0.61 +0.4 4,177,645 + Pf6N2E2_3585 0.82 +0.6 4,177,646 - Pf6N2E2_3585 0.82 -0.5 4,177,696 - Pf6N2E2_3585 0.86 -1.7 4,177,696 - Pf6N2E2_3585 0.86 +1.3 4,177,696 - Pf6N2E2_3585 0.86 -2.0 4,177,717 + Pf6N2E2_3585 0.88 -0.5 4,177,805 + +2.5 4,177,805 + +0.2 4,177,805 + -0.3 4,177,805 + -0.1 4,177,806 - +0.5 4,177,827 + +0.2 4,177,827 + -0.7 4,177,828 - -0.5 4,177,879 + -1.0 4,177,970 + Pf6N2E2_3586 0.12 -0.3 4,177,971 - Pf6N2E2_3586 0.12 -1.5 4,178,106 + Pf6N2E2_3586 0.29 -1.4 4,178,106 + Pf6N2E2_3586 0.29 -1.2 4,178,297 + Pf6N2E2_3586 0.52 -1.2 4,178,297 + Pf6N2E2_3586 0.52 -1.1 4,178,315 + Pf6N2E2_3586 0.54 +0.7 4,178,315 + Pf6N2E2_3586 0.54 -0.6 4,178,315 + Pf6N2E2_3586 0.54 -2.2 4,178,316 - Pf6N2E2_3586 0.54 -2.4 4,178,328 + Pf6N2E2_3586 0.55 -2.4 4,178,328 + Pf6N2E2_3586 0.55 -0.2 4,178,328 + Pf6N2E2_3586 0.55 +0.1 4,178,328 + Pf6N2E2_3586 0.55 -1.5 4,178,328 + Pf6N2E2_3586 0.55 -2.7 4,178,328 + Pf6N2E2_3586 0.55 -1.6 4,178,329 - Pf6N2E2_3586 0.56 -1.5 4,178,329 - Pf6N2E2_3586 0.56 -0.1 4,178,329 - Pf6N2E2_3586 0.56 +0.8 4,178,580 + Pf6N2E2_3586 0.86 -1.7 4,178,580 + Pf6N2E2_3586 0.86 +0.2 4,178,580 + Pf6N2E2_3586 0.86 -0.9 4,178,581 - Pf6N2E2_3586 0.86 -0.8 4,178,652 + -1.8 4,178,652 + +0.3 4,178,699 - -1.0 4,178,799 + -0.5 4,178,981 - +0.7 4,179,080 + Pf6N2E2_3587 0.14 -0.1 4,179,081 - Pf6N2E2_3587 0.15 +0.5 4,179,188 + Pf6N2E2_3587 0.38 -0.1 4,179,188 + Pf6N2E2_3587 0.38 -1.5 4,179,188 + Pf6N2E2_3587 0.38 -0.3 4,179,188 + Pf6N2E2_3587 0.38 +0.4 4,179,189 - Pf6N2E2_3587 0.39 +0.7 4,179,189 - Pf6N2E2_3587 0.39 -0.9 4,179,440 - -0.6 4,179,534 + -0.2 4,179,564 + +2.1 4,179,564 + -0.2 4,179,571 - -1.3 4,179,571 - -0.9 4,179,618 + +1.5 4,179,619 - -1.1 4,179,619 - +0.7 4,179,619 - -3.1 4,179,619 - +0.5 4,179,673 + Pf6N2E2_3588 0.49 +1.2 4,179,673 + Pf6N2E2_3588 0.49 -0.7 4,179,674 - Pf6N2E2_3588 0.49 -0.4
Or see this region's nucleotide sequence